1887

Abstract

In this study, two strains (SCSIO 10187 and SCSIO 10197) were isolated from a sediment sample collected from the South China Sea and characterized by using a polyphasic approach. Growth was observed at 15–35 °C (optimum 28 °C) and pH 5.0–8.0 (optimum pH 6.0). Based on 16S rRNA gene sequence analysis, the strains were identified as members of the genus . Phylogenetic analysis showed that the two strains clustered together and the 16S rRNA gene sequence similarities between them and other members of the genus were 93.2–97.7 %. The menaquinone type was MK-8(H). Major cellular fatty acids were C, Cω9, C, 10-methyl C, C, C and Cω8. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The DNA G+C contents of strains SCSIO 10187 and SCSIO 10197 were 63.7 and 63.2 mol%, respectively. The combined genotypic and phenotypic data showed that the two strains represent a novel species of the genus , for which the name is proposed; the type strain is SCSIO 10187 ( = DSM 45608 = CCTCC AB 2011024), with SCSIO 10197 ( = DSM 45609 = CCTCC AB 2011025) as a reference strain.

Funding
This study was supported by the:
  • National Basic Research Program of China (Award no. 2010CB833801)
  • Knowledge Innovation Program of the Chinese Academy of Sciences (Award KSCX2-EW-G-12)
  • Academic Frontier Project for Young Researchers (Award no. SQ201013)
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2012-10-01
2024-03-28
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References

  1. Bell K. S., Philp J. C., Aw D. W., Christofi N. 1998; The genus Rhodococcus . J Appl Microbiol 85:195–210 [View Article][PubMed]
    [Google Scholar]
  2. Briglia M., Rainey F. A., Stackebrandt E., Schraa G., Salkinoja-Salonen M. S. 1996; Rhodococcus percolatus sp. nov., a bacterium degrading 2,4,6-trichlorophenol. Int J Syst Bacteriol 46:23–30 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [View Article]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  10. Ghosh A., Paul D., Prakash D., Mayilraj S., Jain R. K. 2006; Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. Int J Syst Evol Microbiol 56:1965–1969 [View Article][PubMed]
    [Google Scholar]
  11. Goodfellow M., Alderson G., Chun J. 1998; Rhodococcal systematics: problems and developments. Antonie van Leeuwenhoek 74:3–20 [View Article][PubMed]
    [Google Scholar]
  12. Helmke E., Weyland H. 1984; Rhodococcus marinonascens sp. nov., an actinomycete from the sea. Int J Syst Bacteriol 34:127–138 [View Article]
    [Google Scholar]
  13. Lechevalier H. A., Lechevalier M. P. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–444 [View Article]
    [Google Scholar]
  14. Leifson E. 1960 Atlas of Bacterial Flagellation London: Academic Press;
    [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  16. Li J., Zhao G. Z., Chen H. H., Qin S., Xu L. H., Jiang C. L., Li W. J. 2008; Rhodococcus cercidiphylli sp. nov., a new endophytic actinobacterium isolated from a Cercidiphyllum japonicum leaf. Syst Appl Microbiol 31:108–113 [View Article][PubMed]
    [Google Scholar]
  17. Locci R. 1989; Streptomyces and related genera. In Bergey’s Manual of Systematic Bacteriology vol. 4 pp. 2451–2508 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins Co;
    [Google Scholar]
  18. Matsuyama H., Yumoto I., Kudo T., Shida O. 2003; Rhodococcus tukisamuensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 53:1333–1337 [View Article][PubMed]
    [Google Scholar]
  19. Mayilraj S., Krishnamurthi S., Saha P., Saini H. S. 2006; Rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 56:979–982 [View Article][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  21. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [View Article]
    [Google Scholar]
  22. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [View Article]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  26. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  28. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  29. Takeuchi M., Hatano K., Sedlácek I., Pácová Z. 2002; Rhodococcus jostii sp. nov., isolated from a medieval grave. Int J Syst Evol Microbiol 52:409–413[PubMed]
    [Google Scholar]
  30. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 300:31–36 [CrossRef]
    [Google Scholar]
  31. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  32. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J. 2009; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59:2025–2031 [View Article][PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  34. Wang Z. C., Xu J. J., Li Y., Wang K., Wang Y. Y., Hong Q., Li W. J., Li S. P. 2010; Rhodococcus jialingiae sp. nov., an actinobacterium isolated from sludge of a carbendazim wastewater treatment facility. Int J Syst Evol Microbiol 60:378–381 [View Article][PubMed]
    [Google Scholar]
  35. Warhurst A. M., Fewson C. A. 1994; Biotransformations catalyzed by the genus Rhodococcus . Crit Rev Biotechnol 14:29–73 [View Article][PubMed]
    [Google Scholar]
  36. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey’s Manual of Systematic Bacteriology vol. 4 pp. 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  37. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  38. Xu J. L., He J., Wang Z. C., Wang K., Li W. J., Tang S. K., Li S. P. 2007; Rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. Int J Syst Evol Microbiol 57:2754–2757 [View Article][PubMed]
    [Google Scholar]
  39. Yoon J.-H., Cho Y.-G., Kang S.-S., Kim S. B., Lee S. T., Park Y.-H. 2000; Rhodococcus koreensis sp. nov., a 2,4-dinitrophenol-degrading bacterium. Int J Syst Evol Microbiol 50:1193–1201 [View Article][PubMed]
    [Google Scholar]
  40. Zhang J. L., Zhang Y. M., Xiao C. S., Liu Z. H., Goodfellow M. 2002; Rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. Int J Syst Evol Microbiol 52:2121–2126 [View Article][PubMed]
    [Google Scholar]
  41. Zhang Y. Q., Li W. J., Kroppenstedt R. M., Kim C. J., Chen G. Z., Park D. J., Xu L. H., Jiang C. L. 2005; Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil. Int J Syst Evol Microbiol 55:1133–1137 [View Article][PubMed]
    [Google Scholar]
  42. Zopf W. 1891; Über Ausscheidung von Fettfarbstoffen (Lipochromen) seitens gewisser Spaltpilze. Ber Dtsch Bot Ges 9:22–28
    [Google Scholar]
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