1887

Abstract

A bacterial strain, designated NS31-3, was isolated from the wastewater of a paper mill. Cells of the isolate were obligately anaerobic, non-pigmented, non-motile, Gram-negative, short rods (0.7–1.0×1.4–2.5 µm). The isolate was able to grow on media containing 20 % bile salts. API 20A tests showed that acid was produced from glucose, lactose, sucrose, maltose, -xylose, -arabinose, cellobiose, -mannose, -melezitose, -raffinose, -trehalose, -mannitol, salicin and -sorbitol. The main fermentation products from PYG broth were lactic acid, propionic acid, formic acid and acetic acid. Chemotaxonomic analysis showed that the major fatty acids were anteiso-C, C and iso-C 3-OH and the predominant respiratory quinones were MK-9 and MK-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NS31-3 was related to members of genus (91.2–93.2 % sequence similarity); the isolate had the closest affinity with JCM 9497. The G+C content of the genomic DNA was 37.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain NS31-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NS31-3 ( = JCM 17797  = DSM 24967).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038000-0
2012-11-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/11/2613.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038000-0&mimeType=html&fmt=ahah

References

  1. Bryant M. P. . ( 1972; ). Commentary on the Hungate technique for culture of anaerobic bacteria. . Am J Clin Nutr 25:, 1324–1328.[PubMed]
    [Google Scholar]
  2. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  3. Eggerth A. H. , Gagnon B. H. . ( 1933; ). The bacteroides of human feces. . J Bacteriol 25:, 389–413.[PubMed]
    [Google Scholar]
  4. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  5. Hungate R. E. . ( 1969; ). A roll tube method for cultivation of strict anaerobes. . Methods Microbiol 3B:, 117–132. [CrossRef]
    [Google Scholar]
  6. Johnson J. L. , Moore W. E. C. , Moore L. V. H. . ( 1986; ). Bacteroides caccae sp. nov., Bacteroides merdae sp. nov., and Bacteroides stercoris sp. nov. isolated from human feces. . Int J Syst Bacteriol 36:, 499–501. [CrossRef]
    [Google Scholar]
  7. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kuykendall L. D. , Roy M. A. , O’Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  9. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  10. Rainey F. A. , Ward-Rainey N. , Kroppenstedt R. M. , Stackebrandt E. . ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef] [PubMed]
    [Google Scholar]
  11. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  12. Sakamoto M. , Benno Y. . ( 2006; ). Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov.. Int J Syst Evol Microbiol 56:, 1599–1605. [CrossRef] [PubMed]
    [Google Scholar]
  13. Sakamoto M. , Kitahara M. , Benno Y. . ( 2007; ). Parabacteroides johnsonii sp. nov., isolated from human faeces. . Int J Syst Evol Microbiol 57:, 293–296. [CrossRef] [PubMed]
    [Google Scholar]
  14. Sakamoto M. , Suzuki N. , Matsunaga N. , Koshihara K. , Seki M. , Komiya H. , Benno Y. . ( 2009; ). Parabacteroides gordonii sp. nov., isolated from human blood cultures. . Int J Syst Evol Microbiol 59:, 2843–2847. [CrossRef] [PubMed]
    [Google Scholar]
  15. Shah H. N. . ( 1992; ). The genus Bacteroides and related taxa. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 3593–3607. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  16. Simmon K. E. , Mirrett S. , Reller L. B. , Petti C. A. . ( 2008; ). Genotypic diversity of anaerobic isolates from bloodstream infections. . J Clin Microbiol 46:, 1596–1601. [CrossRef] [PubMed]
    [Google Scholar]
  17. Song Y. , Liu C. , Lee J. , Bolanos M. , Vaisanen M. L. , Finegold S. M. . ( 2005; ). Bacteroides goldsteinii sp. nov.’ isolated from clinical specimens of human intestinal origin. . J Clin Microbiol 43:, 4522–4527. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  19. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tindall B. J. . ( 1989; ). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum . . FEMS Microbiol Lett 60:, 251–254. [CrossRef]
    [Google Scholar]
  21. Widdel F. , Kohring G. W. , Mayer F. . ( 1983; ). Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. . Arch Microbiol 134:, 286–294. [CrossRef]
    [Google Scholar]
  22. Xu X.-W. , Wu Y.-H. , Zhou Z. , Wang C.-S. , Zhou Y.-G. , Zhang H.-B. , Wang Y. , Wu M. . ( 2007; ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57:, 1619–1624. [CrossRef] [PubMed]
    [Google Scholar]
  23. Zhang X.-Q. , Ying Y. , Ye Y. , Xu X.-W. , Zhu X.-F. , Wu M. . ( 2010; ). Thermus arciformis sp. nov., a thermophilic species from a geothermal area. . Int J Syst Evol Microbiol 60:, 834–839. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038000-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038000-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error