1887

Abstract

A bacterial strain, designated NS31-3, was isolated from the wastewater of a paper mill. Cells of the isolate were obligately anaerobic, non-pigmented, non-motile, Gram-negative, short rods (0.7–1.0×1.4–2.5 µm). The isolate was able to grow on media containing 20 % bile salts. API 20A tests showed that acid was produced from glucose, lactose, sucrose, maltose, -xylose, -arabinose, cellobiose, -mannose, -melezitose, -raffinose, -trehalose, -mannitol, salicin and -sorbitol. The main fermentation products from PYG broth were lactic acid, propionic acid, formic acid and acetic acid. Chemotaxonomic analysis showed that the major fatty acids were anteiso-C, C and iso-C 3-OH and the predominant respiratory quinones were MK-9 and MK-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NS31-3 was related to members of genus (91.2–93.2 % sequence similarity); the isolate had the closest affinity with JCM 9497. The G+C content of the genomic DNA was 37.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain NS31-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NS31-3 ( = JCM 17797  = DSM 24967).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 30970002)
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2012-11-01
2020-07-04
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