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Volume 63, Issue Pt_1

gen. nov., sp. nov., a marine bacterium of the family isolated from a tidal flat and emended descriptions of the genera and Free

Abstract

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterium, designated strain SC17, was isolated from sediment of a tidal flat of Suncheon bay in South Korea. Cells were halotolerant, catalase- and oxidase-positive and non-motile rods. Growth of strain SC17 was observed at 5–40 °C (optimum, 25–30 °C), at pH 6.0–8.5 (optimum, pH 7.0) and in the presence of 1–8 % (w/v) NaCl (optimum, 1–2 %). The major cellular fatty acids were iso-C, summed feature 3 (comprising Cω7 and/or Cω6 and/or iso-C 2-OH), iso-C 3-OH, iso-C G and anteiso-C The polar lipid content consisted of phosphatidylethanolamine and unidentified amino lipids and lipids. The G+C content of the genomic DNA was 46.4 mol% and the only respiratory quinone detected was menaquinone-6 (MK-6). Phylogenetic inference based on 16S rRNA gene sequences showed that strain SC17 formed a distinct phyletic lineage within the family and was most closely related to members of the genera and with 95.0–95.8 % sequence similarity. On the basis of phenotypic and molecular features, strain SC17 represents a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is SC17 ( = KACC 16186 = JCM 17789). Emended descriptions of the genera and are also proposed.

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Funding
This study was supported by the:
  • Chung-Ang University Research Scholarship Grant
© 2013 IUMS
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2013-01-01
2024-04-20
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References

  1. Asker D., Beppu T., Ueda K. ( 2007 ). Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan. . Appl Microbiol Biotechnol 77, 383–392. [View Article] [PubMed]
    [Google Scholar]
  2. Bernardet J.-F. ( 2011 ). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. . New York:: Springer;.
     [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y. ( 2006 ). An introduction to the family Flavobacteriaceae . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 455–480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;.
     [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  5. Biebl H., Allgaier M., Tindall B. J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. ( 2005 ). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. . Int J Syst Evol Microbiol 55, 1089–1096. [View Article] [PubMed]
    [Google Scholar]
  6. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  7. Bowman J. P., Nichols D. S. ( 2005 ). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . . Int J Syst Evol Microbiol 55, 1471–1486. [View Article] [PubMed]
    [Google Scholar]
  8. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  9. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 2259–2261. [View Article] [PubMed]
    [Google Scholar]
  10. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
     [Google Scholar]
  11. Felsenstein, J. (2002). phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . In Methods in Enzymology, vol. 1, pp. 138–146. Edited by Colowick S. P., Kaplan N. O. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  13. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770–773. [View Article] [PubMed]
    [Google Scholar]
  14. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. ( 2004 ). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54, 93–97. [View Article] [PubMed]
    [Google Scholar]
  15. Jin H. M., Jeong H., Moon E.-J., Math R. K., Lee K., Kim H.-J., Jeon C. O., Oh T. K., Kim J. F. ( 2011a ). Complete genome sequence of the polycyclic aromatic hydrocarbon-degrading bacterium Alteromonas sp. strain SN2. . J Bacteriol 193, 4292–4293. [View Article] [PubMed]
    [Google Scholar]
  16. Jin H. M., Lee H. J., Kim J. M., Park M. S., Lee K., Jeon C. O. ( 2011b ). Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 1392–1396. [View Article] [PubMed]
    [Google Scholar]
  17. Jung S.-Y., Kang S.-J., Lee M.-H., Lee S.-Y., Oh T.-K., Yoon J.-H. ( 2005 ). Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. . Int J Syst Evol Microbiol 55, 1845–1849. [View Article] [PubMed]
    [Google Scholar]
  18. Jung J. Y., Kim J. M., Jin H. M., Kim S. Y., Park W., Jeon C. O. ( 2011 ). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 1534–1538. [View Article] [PubMed]
    [Google Scholar]
  19. Kim J. M., Lee H. J., Kim S. Y., Song J. J., Park W., Jeon C. O. ( 2010 ). Analysis of fine-scale population structure of “Candidatus Accumulibacter phosphatis” using fluorescence in situ hybridization and flow cytometric sorting. . Appl Environ Microbiol 76, 3825–3835. [View Article] [PubMed]
    [Google Scholar]
  20. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  21. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  22. Lee S. D. ( 2007 ). Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea. . Int J Syst Evol Microbiol 57, 764–769. [View Article] [PubMed]
    [Google Scholar]
  23. Lee S. H., Shim J. K., Kim J. M., Choi H. K., Jeon C. O. ( 2011 ). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . . Int J Syst Evol Microbiol 61, 722–727. [View Article] [PubMed]
    [Google Scholar]
  24. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 1183–1184.[PubMed]
    [Google Scholar]
  25. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. ( 2006 ). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. . J Microbiol 44, 155–161.[PubMed]
    [Google Scholar]
  26. Ludwig W., Euzéby J., Whitman W. B. ( 2010 ). Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 21–24. Edited by Whitman W. . Baltimore:: The Williams & Wilkins Co;. [View Article]
    [Google Scholar]
  27. McCammon S. A., Bowman J. P. ( 2000 ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50, 1055–1063. [View Article] [PubMed]
    [Google Scholar]
  28. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104–117. [View Article]
    [Google Scholar]
  29. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O. ( 2011 ). Luteimonas lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 61, 2729–2733. [View Article] [PubMed]
    [Google Scholar]
  30. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  31. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. . Washington, D.C.:: American Society for Microbiology;.
     [Google Scholar]
  32. Stamatakis A., Ott M., Ludwig T. ( 2005 ). RAxML-OMP: An efficient program for phylogenetic inference on SMPs”. . In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science, vol. 3506, pp. 288–302. Location:: Springer-Verlag;.
     [Google Scholar]
  33. Wang Q., Garrity G. M., Tiedje J. M., Cole J. R. ( 2007 ). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. . Appl Environ Microbiol 73, 5261–5267. [View Article] [PubMed]
    [Google Scholar]
  34. Yang S. J., Cho J. C. ( 2008 ). Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . . Int J Syst Evol Microbiol 58, 315–318. [View Article] [PubMed]
    [Google Scholar]
  35. Yoon J. H., Kang S. J., Lee M. H., Oh T. K. ( 2008 ). Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea. . Int J Syst Evol Microbiol 58, 1892–1895. [View Article] [PubMed]
    [Google Scholar]
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Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana
Int J Syst Evol Microbiol 63, 332 (2013); https://doi.org/10.1099/ijs.0.037846-0
/content/journal/ijsem/10.1099/ijs.0.037846-0
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