A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain BY-1T, was isolated from a soil sample from the city of Qiqihar in Heilongjiang Province, PR China. Strain BY-1T grew optimally at pH 7.0 and 30–35 °C in the presence of 0.5 % (w/v) NaCl. Analysis of 16S rRNA gene sequences revealed that strain BY-1T fell within the radiation of the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas luteola IAM 13000T (99.5 %) and Pseudomonas duriflava HR2T (97.3 %); the levels of sequence similarity with respect to other recognized species of the genus Pseudomonas were <96.7 %. Strain BY-1T showed low DNA–DNA relatedness values with Pseudomonas luteola IAM 13000T (29±3.1 %) and Pseudomonas duriflava HR2T (21±1.5 %). The G+C content of the genomic DNA of strain BY-1T was 55.3 mol%. The major fatty acids were C18 : 1, C16 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major ubiquinone was Q-9. Data obtained in this study indicated that this isolate represents a novel species of the genus Pseudomonas, for which the name Pseudomonas zeshuii sp. nov. is proposed. The type strain is BY-1T ( = KACC 15471T = ACCC 05688T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 30900044)
Fund for the Doctoral Program of Higher Education
(Award 20090097120031)
Social Development Program Fund of Jiangsu Province
(Award BE2011783)
AnzaiY.,
KimH.,
ParkJ.-Y.,
WakabayashiH.,
OyaizuH.2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [View Article][PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed][CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
KodamaK.,
KimuraN.,
KomagataK.1985; Two new species of Pseudomonas: P. oryzihabitans isolated from rice paddy and clinical specimens and P. luteola isolated from clinical specimens. Int J Syst Evol Microbiol 35:467–474
MandelM.,
MarmurJ.1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [View Article]
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47:87–95 [View Article]
OyaizuH.,
KomagataK.1983; Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. J Gen Appl Microbiol 29:17–40 [View Article]
PalleroniN. J.1984; Genus I. Pseudomonas Migula 1894. In Bergey’s Manual of Systematic Bacteriologyvol. 1 pp. 141–199 Edited by
KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
SneathP. H. A.,
StevensM.,
SackinM. J.1981; Numerical taxonomy of Pseudomonas based on published records of substrate utilization. Antonie van Leeuwenhoek 47:423–448 [View Article][PubMed]
SuzukiM.,
NakagawaY.,
HarayamaS.,
YamamotoS.2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
TindallB. J.,
Rosselló-MóraR.,
BusseH.-J.,
LudwigW.,
KämpferP.2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R. et al.1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]