1887

Abstract

A marine, magnetotactic bacterium, designated strain MMS-1, was isolated from mud and water from a salt marsh in Woods Hole, Massachusetts, USA, after enrichment in defined oxygen-concentration/redox-gradient medium. Strain MMS-1 is an obligate microaerophile capable of chemoorganoheterotrophic and chemolithoautotrophic growth. Optimal growth occurred at pH 7.0 and 24–26 °C. Chemolithoautotrophic growth occurred with thiosulfate as the electron donor and autotrophic carbon fixation was via the Calvin–Benson–Bassham cycle. The G+C content of the DNA of strain MMS-1 was 47.2 mol%. Cells were Gram-negative and morphologically variable, with shapes that ranged from that of a lima bean to fully helical. Cells were motile by means of a single flagellum at each end of the cell (amphitrichous). Regardless of whether grown in liquid or semi-solid cultures, strain MMS-1 displayed only polar magnetotaxis and possessed a single chain of magnetosomes containing elongated octahedral crystals of magnetite, positioned along the long axis of the cell. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MMS-1 belongs to the family within the , and is distantly related to species of the genus . Strain MMS-1 is therefore considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is MMS-1 ( = ATCC BAA-1438 = JCM 17960).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.037697-0
2012-10-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/10/2443.html?itemId=/content/journal/ijsem/10.1099/ijs.0.037697-0&mimeType=html&fmt=ahah

References

  1. Bazylinski D. A. , Frankel R. B. . ( 2004; ). Magnetosome formation in prokaryotes. . Nat Rev Microbiol 2:, 217–230. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bazylinski D. A. , Williams T. J. . ( 2007; ). Ecophysiology of magnetotactic bacteria. . In Magnetoreception and Magnetosomes in Bacteria, vol. 3, pp. 37–75. Edited by Schüler D. . . Berlin:: Springer;. [CrossRef]
    [Google Scholar]
  3. Bazylinski D. A. , Frankel R. B. , Jannasch H. W. . ( 1988; ). Anaerobic magnetite production by a marine, magnetotactic bacterium. . Nature 334:, 518–519. [CrossRef]
    [Google Scholar]
  4. Bazylinski D. A. , Dean A. J. , Williams T. J. , Long L. K. , Middleton S. L. , Dubbels B. L. . ( 2004; ). Chemolithoautotrophy in the marine, magnetotactic bacterial strains MV-1 and MV-2. . Arch Microbiol 182:, 373–387. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bazylinski D. A. , Williams T. J. , Lefèvre C. T. , Berg R. J. , Zhang C. L. , Bowser S. S. , Dean A. J. , Beveridge T. J. . ( 2012; ). Magnetococcus marinus gen. nov., sp. nov., a marine, magnetotactic bacterium that represents a novel lineage (Magnetococcaceae fam. nov.; Magnetococcales ord. nov.) at the base of the Alphaproteobacteria . . Int J Syst Evol Microbiol (in press). doi:10.1099/ijs.0.038927-0 [CrossRef] [PubMed]
    [Google Scholar]
  6. Beudeker R. F. , Cannon G. C. , Kuenen J. G. , Shively J. M. . ( 1980; ). Relations between d-ribulose-1,5-bisphosphate carboxylase, carboxysomes and CO2 fixing capacity in the obligate chemolithotroph Thiobacillus neapolitanus grown under different limitations in the chemostat. . Arch Microbiol 124:, 185–189. [CrossRef]
    [Google Scholar]
  7. Blakemore R. P. , Maratea D. , Wolfe R. S. . ( 1979; ). Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. . J Bacteriol 140:, 720–729.[PubMed]
    [Google Scholar]
  8. Dubbels B. L. , DiSpirito A. A. , Morton J. D. , Semrau J. D. , Neto J. N. , Bazylinski D. A. . ( 2004; ). Evidence for a copper-dependent iron transport system in the marine, magnetotactic bacterium strain MV-1. . Microbiology 150:, 2931–2945. [CrossRef] [PubMed]
    [Google Scholar]
  9. Frankel R. B. , Bazylinski D. A. , Johnson M. S. , Taylor B. L. . ( 1997; ). Magneto-aerotaxis in marine coccoid bacteria. . Biophys J 73:, 994–1000. [CrossRef] [PubMed]
    [Google Scholar]
  10. Fukushima R. S. , Weimer P. J. , Kunz D. A. . ( 2002; ). Photocatalytic interaction of resazurin N-oxide with cysteine optimizes preparation of anaerobic culture media. . Anaerobe 8:, 29–34. [CrossRef]
    [Google Scholar]
  11. Gorby Y. A. , Beveridge T. J. , Blakemore R. P. . ( 1988; ). Characterization of the bacterial magnetosome membrane. . J Bacteriol 170:, 834–841.[PubMed]
    [Google Scholar]
  12. Jogler C. , Kube M. , Schübbe S. , Ullrich S. , Teeling H. , Bazylinski D. A. , Reinhardt R. , Schüler D. . ( 2009; ). Comparative analysis of magnetosome gene clusters in magnetotactic bacteria provides further evidence for horizontal gene transfer. . Environ Microbiol 11:, 1267–1277. [CrossRef] [PubMed]
    [Google Scholar]
  13. Katzmann E. , Scheffel A. , Gruska M. , Plitzko J. M. , Schüler D. . ( 2010; ). Loss of the actin-like protein MamK has pleiotropic effects on magnetosome formation and chain assembly in Magnetospirillum gryphiswaldense . . Mol Microbiol 77:, 208–224. [CrossRef] [PubMed]
    [Google Scholar]
  14. Komeili A. , Li Z. , Newman D. K. , Jensen G. J. . ( 2006; ). Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK. . Science 311:, 242–245. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lefèvre C. T. , Bernadac A. , Yu-Zhang K. , Pradel N. , Wu L. F. . ( 2009; ). Isolation and characterization of a magnetotactic bacterial culture from the Mediterranean Sea. . Environ Microbiol 11:, 1646–1657. [CrossRef] [PubMed]
    [Google Scholar]
  16. López-López A. , Pujalte M. J. , Benlloch S. , Mata-Roig M. , Rosselló-Mora R. , Garay E. , Rodríguez-Valera F. . ( 2002; ). Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria . . Int J Syst Evol Microbiol 52:, 1277–1283. [CrossRef] [PubMed]
    [Google Scholar]
  17. Marchesi J. R. , Sato T. , Weightman A. J. , Martin T. A. , Fry J. C. , Hiom S. J. , Dymock D. , Wade W. G. . ( 1998; ). Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. . Appl Environ Microbiol 64:, 795–799.[PubMed]
    [Google Scholar]
  18. Meldrum F. C. , Mann S. , Heywood B. R. , Frankel R. B. , Bazylinski D. A. . ( 1993; ). Electron microscopy study of magnetosomes in two cultured vibrioid magnetotactic bacteria. . Proc Biol Sci 251:, 237–242. [CrossRef]
    [Google Scholar]
  19. Moskowitz B. M. , Bazylinski D. A. , Egli R. , Frankel R. B. , Edwards K. J. . ( 2008; ). Magnetic properties of marine magnetotactic bacteria in a seasonally stratified coastal pond (Salt Pond, MA, USA). . Geophys J Int 174:, 75–92. [CrossRef]
    [Google Scholar]
  20. Murat D. , Quinlan A. , Vali H. , Komeili A. . ( 2010; ). Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. . Proc Natl Acad Sci U S A 107:, 5593–5598. [CrossRef] [PubMed]
    [Google Scholar]
  21. Satomi M. , Kimura B. , Hamada T. , Harayama S. , Fujii T. . ( 2002; ). Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov.. Int J Syst Evol Microbiol 52:, 739–747. [CrossRef] [PubMed]
    [Google Scholar]
  22. Scheffel A. , Gruska M. , Faivre D. , Linaroudis A. , Plitzko J. M. , Schüler D. . ( 2006; ). An acidic protein aligns magnetosomes along a filamentous structure in magnetotactic bacteria. . Nature 440:, 110–114. [CrossRef] [PubMed]
    [Google Scholar]
  23. Schübbe S. , Kube M. , Scheffel A. , Wawer C. , Heyen U. , Meyerdierks A. , Madkour M. H. , Mayer F. , Reinhardt R. , Schüler D. . ( 2003; ). Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island. . J Bacteriol 185:, 5779–5790. [CrossRef] [PubMed]
    [Google Scholar]
  24. Schübbe S. , Williams T. J. , Xie G. , Kiss H. E. , Brettin T. S. , Martinez D. , Ross C. A. , Schüler D. , Cox B. L. . & other authors ( 2009; ). Complete genome sequence of the chemolithoautotrophic marine magnetotactic coccus strain MC-1. . Appl Environ Microbiol 75:, 4835–4852. [CrossRef] [PubMed]
    [Google Scholar]
  25. Simmons S. L. , Sievert S. M. , Frankel R. B. , Bazylinski D. A. , Edwards K. J. . ( 2004; ). Spatiotemporal distribution of marine magnetotactic bacteria in a seasonally stratified coastal salt pond. . Appl Environ Microbiol 70:, 6230–6239. [CrossRef] [PubMed]
    [Google Scholar]
  26. Spiridonova E. M. , Berg I. A. , Kolganova T. V. , Ivanovskiľ R. N. , Kuznetsov B. B. , Turova T. P. . ( 2004; ). [An oligonucleotide primer system for amplification of the ribulose-1,5-bisphosphate carboxylase/oxygenase genes of bacteria of various taxonomic groups]. . Mikrobiologiia 73:, 377–387 (in Russian).[PubMed]
    [Google Scholar]
  27. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  28. Williams T. J. , Zhang C. L. , Scott J. H. , Bazylinski D. A. . ( 2006; ). Evidence for autotrophy via the reverse tricarboxylic acid cycle in the marine magnetotactic coccus strain MC-1. . Appl Environ Microbiol 72:, 1322–1329. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wolfe R. S. , Thauer R. K. , Pfennig N. A. . ( 1987; ). A ‘capillary racetrack’ method for isolation of magnetotactic bacteria. . FEMS Microbiol Ecol 45:, 31–35. [CrossRef]
    [Google Scholar]
  30. Zeytuni N. , Ozyamak E. , Ben-Harush K. , Davidov G. , Levin M. , Gat Y. , Moyal T. , Brik A. , Komeili A. , Zarivach R. . ( 2011; ). Self-recognition mechanism of MamA, a magnetosome-associated TPR-containing protein, promotes complex assembly. . Proc Natl Acad Sci U S A 108:, E480–E487. [CrossRef] [PubMed]
    [Google Scholar]
  31. Zhang C. L. , Fouke B. W. , Bonheyo G. , Peacock A. D. , White D. C. , Huang Y. , Romanek C. S. . ( 2004; ). Lipid biomarkers and carbon-isotopes of modern travertine deposits (Yellowstone National Park, USA): implications for biogeochemical dynamics in hot-spring systems. . Geochim Cosmochim Acta 68:, 3157–3169.[CrossRef]
    [Google Scholar]
  32. Zhu K. , Pan H. , Li J. , Yu-Zhang K. , Zhang S. D. , Zhang W. Y. , Zhou K. , Yue H. , Pan Y. . & other authors ( 2010; ). Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea. . Res Microbiol 161:, 276–283. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.037697-0
Loading
/content/journal/ijsem/10.1099/ijs.0.037697-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error