1887

Abstract

The taxonomic positions of two Gram-staining-negative, psychrophilic bacteria, which were isolated from alpine glacier cryoconite and designated strains Cr4-12 and Cr4-35, were investigated using a polyphasic approach. Both novel strains contained ubiquinone Q-8 as the sole quinone, summed feature 3 (Cω7 and/or Cω6) and C as the dominant cellular fatty acids, putrescine and 2-hydroxyputrescine as the major polyamines, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C contents of strains Cr4-12 and Cr4-35 were 61.3 mol% and 60.7 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains belonged to the genus . Although the 16S rRNA gene sequences of strains Cr4-12 and Cr4-35 were very similar (98.7 % sequence similarity), hybridizations indicated a DNA–DNA relatedness value of only 26.9 % between the two novel strains. In pairwise comparisons with the type strains of recognized species, strains Cr4-12 and Cr4-35 showed 16S rRNA gene sequence similarities of 96.4–98.5 % and 96.5–98.4 %, respectively. Based on the phenotypic and phylogenetic evidence and DNA–DNA relatedness data, strains Cr4-12 and Cr4-35 represent two novel species within the genus , for which the names sp. nov. and sp. nov., respectively, are proposed. The type strain of sp. nov. is Cr4-12 ( = DSM 24062  = LMG 26049  = KACC 15089) and that of sp. nov. is Cr4-35 ( = DSM 24248  = LMG 26050  = KACC 15090).

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2012-11-01
2019-10-22
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References

  1. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  2. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  5. Darcy J. L., Lynch R. C., King A. J., Robeson M. S., Schmidt S. K.. ( 2011;). Global distribution of Polaromonas phylotypes – evidence for a highly successful dispersal capacity. . PLoS ONE 6:, e23742. [CrossRef][PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  7. Feller G., Gerday C.. ( 2003;). Psychrophilic enzymes: hot topics in cold adaptation. . Nat Rev Microbiol 1:, 200–208. [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  9. Foght J., Aislabie J.. ( 2005;). Enumeration of soil microorganisms. . In Manual of Soil Analysis, pp. 261–280. Edited by Margesin R., Schinner F... Berlin, Heidelberg:: Springer-Verlag;.
    [Google Scholar]
  10. Huß V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  11. Irgens R. L., Gosink J. J., Staley J. T.. ( 1996;). Polaromonas vacuolata gen. nov., sp. nov., a psychrophilic, marine, gas vacuolate bacterium from Antarctica. . Int J Syst Bacteriol 46:, 822–826. [CrossRef][PubMed]
    [Google Scholar]
  12. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L.. ( 2004;). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54:, 93–97. [CrossRef][PubMed]
    [Google Scholar]
  13. Kämpfer P., Busse H.-J., Falsen E.. ( 2006;). Polaromonas aquatica sp. nov., isolated from tap water. . Int J Syst Evol Microbiol 56:, 605–608. [CrossRef][PubMed]
    [Google Scholar]
  14. Margesin R.. ( 2009;). Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. . Extremophiles 13:, 257–262. [CrossRef][PubMed]
    [Google Scholar]
  15. Margesin R., Schinner F.. ( 1997;). Bioremediation of diesel-oil contaminated alpine soils at low temperatures. . Appl Microbiol Biotechnol 47:, 462–468. [CrossRef]
    [Google Scholar]
  16. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F.. ( 2003;). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. . Extremophiles 7:, 451–458. [CrossRef][PubMed]
    [Google Scholar]
  17. Mattes T. E., Alexander A. K., Richardson P. M., Munk A. C., Han C. S., Stothard P., Coleman N. V.. ( 2008;). The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology. . Appl Environ Microbiol 74:, 6405–6416. [CrossRef][PubMed]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Morita R. Y.. ( 1975;). Psychrophilic bacteria. . Bacteriol Rev 39:, 144–167.[PubMed]
    [Google Scholar]
  20. Reasoner D. J., Geldreich E. E.. ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  21. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sambrook J., Frisch E. F., Maniatis T.. ( 1989;). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  23. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  24. Schlegel H. G.. ( 1992;). Allgemeine Mikrobiologie. Stuttgart:: Georg Thieme;.
    [Google Scholar]
  25. Sizova M., Panikov N.. ( 2007;). Polaromonas hydrogenivorans sp. nov., a psychrotolerant hydrogen-oxidizing bacterium from Alaskan soil. . Int J Syst Evol Microbiol 57:, 616–619. [CrossRef][PubMed]
    [Google Scholar]
  26. Stolz A., Busse H.-J., Kämpfer P.. ( 2007;). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:, 572–576. [CrossRef][PubMed]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  29. Tindall B. J.. ( 1990a;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  30. Tindall B. J.. ( 1990b;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  32. Weon H. Y., Yoo S. H., Hong S. B., Kwon S. W., Stackebrandt E., Go S. J., Koo B. S.. ( 2008;). Polaromonas jejuensis sp. nov., isolated from soil in Korea. . Int J Syst Evol Microbiol 58:, 1525–1528. [CrossRef][PubMed]
    [Google Scholar]
  33. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology (English translation of Microbiologiia) 16:, 176–178.
    [Google Scholar]
  34. Yagi J. M., Sims D., Brettin T., Bruce D., Madsen E. L.. ( 2009;). The genome of Polaromonas naphthalenivorans strain CJ2, isolated from coal tar-contaminated sediment, reveals physiological and metabolic versatility and evolution through extensive horizontal gene transfer. . Environ Microbiol 11:, 2253–2270. [CrossRef][PubMed]
    [Google Scholar]
  35. Zhang D.-C., Schinner F., Margesin R.. ( 2010;). Pedobacter bauzanensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60:, 2592–2595. [CrossRef][PubMed]
    [Google Scholar]
  36. Zhang D.-C., Busse H.-J., Liu H.-C., Zhou Y.-G., Schinner F., Margesin R.. ( 2011;). Sphingomonas glacialis sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. . Int J Syst Evol Microbiol 61:, 587–591. [CrossRef][PubMed]
    [Google Scholar]
  37. Zhang D.-C., Redzic M., Liu H.-C., Zhou Y.-G., Schinner F., Margesin R.. ( 2012;). Devosia psychrophila sp. nov. and Devosia glacialis sp. nov., from alpine glacier cryoconite, and an emended description of the genus Devosia. . Int J Syst Evol Microbiol 62:, 710–715. [CrossRef][PubMed]
    [Google Scholar]
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