1887

Abstract

A Gram-negative, aerobic and non-motile rod, designated Y4, was isolated from a cucumber leaf from Pinggu District, east Beijing, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Y4 was most closely related to RIB1-20 (96.7 % 16S rRNA gene sequence similarity). DNA–DNA relatedness between strain Y4 and RIB1-20 was 42.5±3.9 %. The predominant fatty acids were iso-C, iso-Cω9, iso-C and iso-C. The major ubiquinone was Q-8. The DNA G+C content of the type strain was 69.9 mol%. Based on the evidence above, strain Y4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y4 ( = CGMCC 1.10821 = KCTC 23627).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31170035 and 30870055, 30871464)
  • Natural Science Foundation of Shandong (Award ZR2010CQ042)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.037549-0
2012-12-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/2916.html?itemId=/content/journal/ijsem/10.1099/ijs.0.037549-0&mimeType=html&fmt=ahah

References

  1. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N. 2008; Luteimonas marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58:2904–2908 [View Article][PubMed]
    [Google Scholar]
  2. Chou J.-H., Cho N.-T., Arun A. B., Young C.-C., Chen W.-M. 2008; Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. Int J Syst Evol Microbiol 58:2051–2055 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T. 1995; Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococcus profundus DT5432. Appl Environ Microbiol 61:1502–1506[PubMed]
    [Google Scholar]
  6. Collins M. D., Howarth O. W., Grund E., Kroppenstedt R. M. 1987; Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis . FEMS Microbiol Lett 41:35–39 [View Article]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  8. Dong X., Xin Y., Jian W., Liu X., Ling D. 2000; Bifidobacterium thermacidophilum sp. nov., isolated from an anaerobic digester. Int J Syst Evol Microbiol 50:119–125 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  10. Finkmann W., Altendorf K., Stackebrandt E., Lipski A. 2000; Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov.. Int J Syst Evol Microbiol 50:273–282 [View Article][PubMed]
    [Google Scholar]
  11. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  12. Heery D. M., Gannon F., Powell R. 1990; A simple method for subcloning DNA fragments from gel slices. Trends Genet 6:173 [View Article][PubMed]
    [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  15. Mackie T., McCartney J. E. 1989; Organization of the clinical laboratory. In Mackie and McCartney’s Practical Medical Microbiology, 13th edn. p. 21 Edited by Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P. Edinburgh: Churchill Livingstone;
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  17. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758[PubMed]
    [Google Scholar]
  18. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
    [Google Scholar]
  19. Roh S. W., Kim K.-H., Nam Y.-D., Chang H.-W., Kim M.-S., Yoon J.-H., Oh H.-M., Bae J.-W. 2008; Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 46:525–529 [View Article][PubMed]
    [Google Scholar]
  20. Rubini M. R., Silva-Ribeiro R. T., Pomella A. W., Maki C. S., Araújo W. L., Dos Santos D. R., Azevedo J. L. 2005; Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of witches’ broom disease. Int J Biol Sci 1:24–33 [View Article][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  22. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.;
    [Google Scholar]
  23. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. 1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156 [View Article]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  28. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  29. Young C. C., Kämpfer P., Chen W. M., Yen W. S., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Lin K. Y., Chou J. H. 2007; Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. Int J Syst Evol Microbiol 57:741–744 [View Article][PubMed]
    [Google Scholar]
  30. Zhang D. C., Liu H. C., Xin Y. H., Zhou Y. G., Schinner F., Margesin R. 2010; Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. Int J Syst Evol Microbiol 60:1581–1584 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.037549-0
Loading
/content/journal/ijsem/10.1099/ijs.0.037549-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error