1887

Abstract

A Gram-negative, aerobic and non-motile rod, designated Y4, was isolated from a cucumber leaf from Pinggu District, east Beijing, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Y4 was most closely related to RIB1-20 (96.7 % 16S rRNA gene sequence similarity). DNA–DNA relatedness between strain Y4 and RIB1-20 was 42.5±3.9 %. The predominant fatty acids were iso-C, iso-Cω9, iso-C and iso-C. The major ubiquinone was Q-8. The DNA G+C content of the type strain was 69.9 mol%. Based on the evidence above, strain Y4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y4 ( = CGMCC 1.10821 = KCTC 23627).

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2012-12-01
2019-10-17
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References

  1. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N.. ( 2008;). Luteimonas marina sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 58:, 2904–2908. [CrossRef][PubMed]
    [Google Scholar]
  2. Chou J.-H., Cho N.-T., Arun A. B., Young C.-C., Chen W.-M.. ( 2008;). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. . Int J Syst Evol Microbiol 58:, 2051–2055. [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Goodfellow M.. ( 1995;). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45:, 240–245. [CrossRef][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  5. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T.. ( 1995;). Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococcus profundus DT5432. . Appl Environ Microbiol 61:, 1502–1506.[PubMed]
    [Google Scholar]
  6. Collins M. D., Howarth O. W., Grund E., Kroppenstedt R. M.. ( 1987;). Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis. . FEMS Microbiol Lett 41:, 35–39. [CrossRef]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  8. Dong X., Xin Y., Jian W., Liu X., Ling D.. ( 2000;). Bifidobacterium thermacidophilum sp. nov., isolated from an anaerobic digester. . Int J Syst Evol Microbiol 50:, 119–125. [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Finkmann W., Altendorf K., Stackebrandt E., Lipski A.. ( 2000;). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov.. Int J Syst Evol Microbiol 50:, 273–282. [CrossRef][PubMed]
    [Google Scholar]
  11. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  12. Heery D. M., Gannon F., Powell R.. ( 1990;). A simple method for subcloning DNA fragments from gel slices. . Trends Genet 6:, 173. [CrossRef][PubMed]
    [Google Scholar]
  13. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  14. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  15. Mackie T., McCartney J. E.. ( 1989;). Organization of the clinical laboratory. . In Mackie and McCartney’s Practical Medical Microbiology, , 13th edn., p. 21. Edited by Collee J. G., Duguid J. P., Fraser A. G., Marmion B. P... Edinburgh:: Churchill Livingstone;.
    [Google Scholar]
  16. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  17. Powers E. M.. ( 1995;). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  18. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef][PubMed]
    [Google Scholar]
  19. Roh S. W., Kim K.-H., Nam Y.-D., Chang H.-W., Kim M.-S., Yoon J.-H., Oh H.-M., Bae J.-W.. ( 2008;). Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. . J Microbiol 46:, 525–529. [CrossRef][PubMed]
    [Google Scholar]
  20. Rubini M. R., Silva-Ribeiro R. T., Pomella A. W., Maki C. S., Araújo W. L., Dos Santos D. R., Azevedo J. L.. ( 2005;). Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of witches’ broom disease. . Int J Biol Sci 1:, 24–33. [CrossRef][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sasser M..( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
    [Google Scholar]
  23. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V.. ( 1986;). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. . J Microbiol Methods 5:, 139–156. [CrossRef]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  28. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  29. Young C. C., Kämpfer P., Chen W. M., Yen W. S., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Lin K. Y., Chou J. H.. ( 2007;). Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. . Int J Syst Evol Microbiol 57:, 741–744. [CrossRef][PubMed]
    [Google Scholar]
  30. Zhang D. C., Liu H. C., Xin Y. H., Zhou Y. G., Schinner F., Margesin R.. ( 2010;). Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. . Int J Syst Evol Microbiol 60:, 1581–1584. [CrossRef][PubMed]
    [Google Scholar]
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