1887

Abstract

A Gram-negative, short-rod-shaped, motile, non-spore-forming and poly-β-hydroxybutyrate-accumulating bacterial strain, designated wsw-7, was isolated from a freshwater shrimp culture pond in Taiwan and was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest relatives of strain wsw-7 were 332, T7 and ATCC 15668, with sequence similarities of 98.5, 98.4 and 97.9 %, respectively. Phylogenetic trees obtained with 16S rRNA gene sequences or the polyhydroxyalkanoate synthase () gene sequences revealed that strain wsw-7 and these three closest relatives formed an independent phylogenetic clade within the order . Strain wsw-7 contained summed feature 3 (comprising Cω7 and/or Cω6), C and Cω7 as predominant fatty acids. The major isoprenoid quinone was Q-8 and the DNA G+C content was 67.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and several uncharacterized phospholipids. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain wsw-7 represents a novel species in the genus for which the name sp. nov. is proposed; the type strain is wsw-7 ( = BCRC 80212 = LMG 25724).

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2012-10-01
2021-10-18
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