1887

Abstract

Four strains, designated JPY-345, JPY-347, JPY-366 and JPY-581, were isolated from nitrogen-fixing nodules on the roots of two species of , and , that are native to North East Brazil, and their taxonomic positions were investigated by using a polyphasic approach. All four strains grew at 15–43 °C (optimum 35 °C), at pH 4–7 (optimum pH 5) and with 0–2 % (w/v) NaCl (optimum 0 % NaCl). On the basis of 16S rRNA gene sequence analysis, strain JPY-345 showed 97.3 % sequence similarity to the closest related species GP25-8, 97.3 % sequence similarity to ATCC25418 and 97.1 % sequence similarity to KP23. The predominant fatty acids of the strains were Cω7 (36.1 %), C (19.8 %) and summed feature 3, comprising Cω7 and/or Cω6 (11.5 %). The major isoprenoid quinone was Q-8 and the DNA G+C content of the strains was 64.2–65.7 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. DNA–DNA hybridizations between the novel strain and recognized species of the genus yielded relatedness values of <51.8 %. On the basis of 16S rRNA and gene sequence similarities and chemotaxonomic and phenotypic data, the four strains represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JPY-345 ( = LMG 26032  = BCRC 80258  = KCTC 23309).

Funding
This study was supported by the:
  • , National Science Council, Taipei, Taiwan, Republic of China , (Award NSC 96-2313-B-022-001-MY3)
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2012-09-01
2021-02-28
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