1887

Abstract

Several intermediate-growing, photochromogenic bacteria were isolated from sphagnum peat bogs in northern Minnesota, USA. Acid-fast staining and 16S rRNA gene sequence analysis placed these environmental isolates in the genus , and colony morphologies and PCR restriction analysis patterns of the isolates were similar. Partial sequences of and from these isolates showed that ATCC BAA-1242 was the closest mycobacterial relative, and common biochemical characteristics and antibiotic susceptibilities existed between the isolates and ATCC BAA-1242. However, compared to nonchromogenic ATCC BAA-1242, the environmental isolates were photochromogenic, had a different mycolic acid profile and had reduced cell-surface hydrophobicity in liquid culture. The data reported here support the conclusion that the isolates are representatives of a novel mycobacterial species, for which the name sp. nov. is proposed. The type strain is DL49 ( = DSM 45633 = JCM 17932 = NCCB 100399).

Funding
This study was supported by the:
  • University of Minnesota Duluth
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.037291-0
2013-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/1/124.html?itemId=/content/journal/ijsem/10.1099/ijs.0.037291-0&mimeType=html&fmt=ahah

References

  1. Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. ( 2007 ). Methods for General and Molecular Microbiology, , 3rd edn.. Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  2. Cassidy P. M., Hedberg K., Saulson A., McNelly E., Winthrop K. L. . ( 2009 ). Nontuberculous mycobacterial disease prevalence and risk factors: a changing epidemiology. . Clin Infect Dis 49, e124e129. [View Article] [PubMed]
    [Google Scholar]
  3. Chilima B. Z., Clark I. M., Floyd S., Fine P. E. M., Hirsch P. R. . ( 2006 ). Distribution of environmental mycobacteria in Karonga District, northern Malawi. . Appl Environ Microbiol 72, 23432350. [View Article] [PubMed]
    [Google Scholar]
  4. Cloud J. L., Meyer J. J., Pounder J. I., Jost K. C. Jr, Sweeney A., Carroll K. C., Woods G. L. . ( 2006 ). Mycobacterium arupense sp. nov., a non-chromogenic bacterium isolated from clinical specimens. . Int J Syst Evol Microbiol 56, 14131418. [View Article] [PubMed]
    [Google Scholar]
  5. Cook J. L. . ( 2010 ). Nontuberculous mycobacteria: opportunistic environmental pathogens for predisposed hosts. . Br Med Bull 96, 4559. [View Article] [PubMed]
    [Google Scholar]
  6. Durnez L., Eddyani M., Mgode G. F., Katakweba A., Katholi C. R., Machang’u R. R., Kazwala R. R., Portaels F., Leirs H. . ( 2008 ). First detection of mycobacteria in African rodents and insectivores, using stratified pool screening. . Appl Environ Microbiol 74, 768773. [View Article] [PubMed]
    [Google Scholar]
  7. Falkinham J. O. III ( 2009 ). Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment. . J Appl Microbiol 107, 356367. [View Article] [PubMed]
    [Google Scholar]
  8. Falkinham J. O. . ( 2010 ). Impact of human activities on the ecology of nontuberculous mycobacteria. . Future Microbiol 5, 951960. [View Article] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  10. Kazda J. . ( 2000 ). The Ecology of Mycobacteria. The Netherlands:: Kluwer Academic Publishers;. [View Article]
    [Google Scholar]
  11. Kimura M. . ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  12. Leao S. C., Martin A., Mejia G. I., Palomino J. C., Robledo J., Telles M. A. S., Portaels F. . ( 2004 ). Practical Handbook for the Phenotypic and Genotypic Identification of Mycobacteria. Bruges, Belgium:: Vanden Broelle;.
    [Google Scholar]
  13. Lee E. S., Lee M. Y., Han S. H., Ka J. O. . ( 2008 ). Occurrence and molecular differentiation of environmental mycobacteria in surface waters. . J Microbiol Biotechnol 18, 12071215.[PubMed]
    [Google Scholar]
  14. Martin E., Kämpfer P., Jäckel U. . ( 2010 ). Quantification and identification of culturable airborne bacteria from duck houses. . Ann Occup Hyg 54, 217227. [View Article] [PubMed]
    [Google Scholar]
  15. Masaki T., Ohkusu K., Hata H., Fujiwara N., Iihara H., Yamada-Noda M., Nhung P. H., Hayashi M., Asano Y. et al. ( 2006 ). Mycobacterium kumamotonense sp. nov. recovered from clinical specimen and the first isolation report of Mycobacterium arupense in Japan: novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex. . Microbiol Immunol 50, 889897.[PubMed] [CrossRef]
    [Google Scholar]
  16. Mendum T. A., Chilima B. Z., Hirsch P. R. . ( 2000 ). The PCR amplification of non-tuberculous mycobacterial 16S rRNA sequences from soil. . FEMS Microbiol Lett 185, 189192. [View Article] [PubMed]
    [Google Scholar]
  17. Neonakis I. K., Gitti Z., Kontos F., Baritaki S., Petinaki E., Baritaki M., Liakou V., Zerva L., Spandidos D. A. . ( 2010 ). Mycobacterium arupense pulmonary infection: antibiotic resistance and restriction fragment length polymorphism analysis. . Indian J Med Microbiol 28, 173176. [View Article] [PubMed]
    [Google Scholar]
  18. Pauls R. J., Turenne C. Y., Wolfe J. N., Kabani A. . ( 2003 ). A high proportion of novel mycobacteria species identified by 16S rDNA analysis among slowly growing AccuProbe-negative strains in a clinical setting. . Am J Clin Pathol 120, 560566. [View Article] [PubMed]
    [Google Scholar]
  19. Radomski N., Cambau E., Moulin L., Haenn S., Moilleron R., Lucas F. S. . ( 2010 ). Comparison of culture methods for isolation of nontuberculous mycobacteria from surface waters. . Appl Environ Microbiol 76, 35143520. [View Article] [PubMed]
    [Google Scholar]
  20. Saitou N., Nei M. . ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  21. Slany M., Svobodova J., Ettlova A., Slana I., Mrlik V., Pavlik I. . ( 2010 ). Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal, and environmental samples. . Vet Med (Praha) 55, 369376.
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S. . ( 2007 ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  23. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. . ( 1993 ). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. . J Clin Microbiol 31, 175178.[PubMed]
    [Google Scholar]
  24. Tierno P. M. Jr, Milstoc M. . ( 1981 ). Combined modified heat-stable acid phosphatase and 68°C catalase test for differentiation of mycobacteria. . J Clin Microbiol 13, 998999.[PubMed]
    [Google Scholar]
  25. Vaneechoutte M., De Beenhouwer H., Claeys G., Verschraegen G., De Rouck A., Paepe N., Elaichouni A., Portaels F. . ( 1993 ). Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis. . J Clin Microbiol 31, 20612065.[PubMed]
    [Google Scholar]
  26. Yamada-Noda M., Ohkusu K., Hata H., Shah M. M., Nhung P. H., Sun X. S., Hayashi M., Ezaki T. . ( 2007 ). Mycobacterium species identification – a new approach via dnaJ gene sequencing. . Syst Appl Microbiol 30, 453462. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.037291-0
Loading
/content/journal/ijsem/10.1099/ijs.0.037291-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error