1887

Abstract

Strain 211020 was isolated from rhizosphere soil of in a mangrove in Hainan, China. The strain produced longitudinal pair spores branching from aerial hyphae. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus , exhibiting the highest 16S rRNA gene sequence similarity (98.75 %) to JCM 10267 with a low DNA–DNA relatedness value (13±0.6 %). The isolate contained -diaminopimelic acid as the diagnostic diamino acid but madurose was not detected. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H), and the major fatty acids were iso-C, iso-C and C. The phospholipid profile of strain 211020 comprised phosphatidylinositol mannoside, phosphatidylethanolamine, diphosphatidylglycerol and phospholipids of unknown structure containing glucosamine. The DNA G+C content was 70.8 mol%. On the basis of phenotypic and genotypic data, strain 211020 can be distinguished as a novel species of the genus , for which the name sp. nov., is proposed. The type strain is 211020 ( = CGMCC 4.5595 = DSM 45428).

Funding
This study was supported by the:
  • , Chinese National Programs for High Technology Research and Development , (Award 2007AA09Z415)
  • , National Natural Science Foundation of China , (Award 30560005)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.037267-0
2012-10-01
2021-01-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/10/2430.html?itemId=/content/journal/ijsem/10.1099/ijs.0.037267-0&mimeType=html&fmt=ahah

References

  1. Arai T. 1975 Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  2. Boondaeng A., Ishida Y., Tamura T., Tokuyama S., Kitpreechavanich V. 2009; Microbispora siamensis sp. nov., a thermotolerant actinomycete isolated from soil. Int J Syst Evol Microbiol 59:3136–3139 [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  4. Cross T. 1974; Genus V. Microbispora Nonomura and Ohara 1957, 307. In Bergey's Manual of Determinative Bacteriology, 8th edn. pp. 859–861 Edited by Buchanan R. E., Gibbons N. E. Baltimore: The Williams & Wilkins Co;
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Goodfellow M., Kumar Y., Labeda D. P., Sembiring L. 2007; The Streptomyces violaceusniger clade: a home for Streptomycetes with rugose ornamented spores. Antonie van Leeuwenhoek 92:173–199 [CrossRef][PubMed]
    [Google Scholar]
  7. Kelly K. L. 1964 Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  8. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  9. Kroppenstedt R. M., Stackebrandt E., Goodfellow M. 1990; Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora . Syst Appl Microbiol 13:148–160 [CrossRef]
    [Google Scholar]
  10. Lechevalier H. A. 1965; Priority of the generic name Microbispora over Waksmania and Thermopolyspora . International Bulletin of Bacteriological Nomenclature and Taxonomy 15:139–142 [CrossRef]
    [Google Scholar]
  11. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, Special Publication 6 pp. 227–291 Edited by Dietz A., Thayer D. W. Arlington, VA: Society of Industrial Microbiology;
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. K. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  14. Miyadoh S., Tohyama H., Amano S., Shomura T., Niida T. 1985; Microbispora viridis, a new species of Actinomycetales. Int J Syst Bacteriol 35:281–284 [CrossRef]
    [Google Scholar]
  15. Miyadoh S., Amano S., Tohyama H., Shomura T. 1990; A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea . J Gen Microbiol 136:1905–1913[PubMed] [CrossRef]
    [Google Scholar]
  16. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [CrossRef][PubMed]
    [Google Scholar]
  17. Nonomura H., Ohara Y. 1957; Distribution of actinomycetes in soil. II. Microbispora, a new genus of the Streptomycetaceae. J Ferment Technol 35:307–311
    [Google Scholar]
  18. Nonomura H., Ohara Y. 1960; Distribution of the actinomycetes in soil. IV. The isolation and classification of the genus Microbispora . J Ferment Technol 38:401–405
    [Google Scholar]
  19. Nonomura H., Ohara Y. 1969; Distribution of actinomycetes in soil. VII. A culture method effective for both preferential isolation and enumeration of Microbispora and Streptosporangium strains in soil. (Part 2). Classification of the isolates. J Ferment Technol 47:701–709
    [Google Scholar]
  20. Nonomura H., Ohara Y. 1971; Distribution of the actinomycetes in soil. IX. New species of the genus Microbispora and Microtetraspora and their isolation methods. J Ferment Technol 49:887–894
    [Google Scholar]
  21. Oliver J. D., Colwell R. R. 1973; Extractable lipids of gram-negative marine bacteria: fatty-acid composition. Int J Syst Bacteriol 23:442–458 [CrossRef]
    [Google Scholar]
  22. Pospiech A., Neumann B. 1995; A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends Genet 11:217–218 [CrossRef][PubMed]
    [Google Scholar]
  23. Rao V. A., Prabhu K. K., Sridhar B. P., Venkateswarlu A., Actor P. 1987; Two new species of Microbispora from Indian soils: Microbispora karnatakensis sp. nov. and Microbispora indica sp. nov.. Int J Syst Bacteriol 37:181–185 [CrossRef]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: Microbial ID.
  26. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  28. Wang Y., Zhang Z., Ruan J. 1996; A proposal to transfer Microbispora bispora (Lechevalier 1965) to a new genus, Thermobispora gen. nov., as Thermobispora bispora comb. nov.. Int J Syst Bacteriol 46:933–938 [CrossRef][PubMed]
    [Google Scholar]
  29. Wang C., Xu X. X., Qu Z., Wang H. L., Lin H. P., Xie Q. Y., Ruan J. S., Hong K. 2011a; Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 61:320–324 [CrossRef][PubMed]
    [Google Scholar]
  30. Wang F., Xu X. X., Qu Z., Wang C., Lin H. P., Xie Q. Y., Ruan J. S., Sun M., Hong K. 2011b; Nonomuraea wenchangensis sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 61:1304–1308 [CrossRef][PubMed]
    [Google Scholar]
  31. Williams S. T., Cross T. 1971; Actinomycetes. In Methods in Microbiology vol. 4 pp. 295–334 Edited by Booth C. London: Academic Press;
    [Google Scholar]
  32. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
    [Google Scholar]
  33. Xu X. X., Lin H. P., Ruan J. S., Hong K. 2009; [Selective Isolation of Microbispora from Rhizhosphere Soil of Mangrove Plants]. Microbiology 32:1299–1304 (in Chinese)
    [Google Scholar]
  34. Zhang Z., Wang Y., Ruan J. 1998; Reclassification of Thermomonospora and Microtetraspora . Int J Syst Bacteriol 48:411–422[PubMed] [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.037267-0
Loading
/content/journal/ijsem/10.1099/ijs.0.037267-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error