1887

Abstract

A bacterial strain, designated NSW-5, was isolated from a water sample taken from Niao-Song Wetland Park in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain NSW-5 were strictly aerobic, Gram-stain-negative, non-motile and polymorphic, being straight, vibrioid, curved and spiral-shaped rods surrounded by a thick capsule and forming light pink-coloured colonies. Some rings consisting of several cells were present. Growth occurred at 10–40 °C (optimum, 25 °C), with 0–3.0 % NaCl (optimum, 0 %) and at pH 6.0–8.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NSW-5 belonged to the genus with sequence similarities of 98.6, 98.0 and 97.3 % with NO-502, TW5 and TNR-18, respectively. The predominant cellular fatty acids were summed feature 3 (Cω7 and/or Cω6; 20.8 %), C (14.6 %), iso-C (13.8 %), Cω5 (12.5 %) and C (11.4 %), and the only respiratory quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized glycolipids, aminolipids, phospholipids and aminophospholipids. The DNA G+C content of strain NSW-5 was 44.1 mol%. The DNA–DNA relatedness of strain NSW-5 with respect to recognized species of the genus was less than 70 %. On the basis of phylogenetic inference and phenotypic data, strain NSW-5 should be classified as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is NSW-5 ( = KCTC 23307 = BCRC 80260). Emended descriptions of the genus and of , and are also proposed.

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2013-01-01
2020-07-13
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