1887

Abstract

Four Gram-stain-positive, aerobic bacterial strains isolated from a pharmaceutical clean room (strain WS4616), a dessert milk product (strain WS4617) and from raw milk (strains WS4623 and WS4624) were characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA and gene sequences showed that they formed a distinct lineage within the family . Similarity values between 16S rRNA gene sequences of the four novel strains and the type species of all genera belonging to the family were 89.2–94.1 %. The major cellular fatty acid was anteiso-C and the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Respiratory quinones were MK-8(H) and MK-9(H). The cell-wall peptidoglycan of type A3γ contained -diaminopimelic acid, alanine, glycine and glutamic acid. The G+C content of the genomic DNA of strain WS4616 was 67.7 mol%. The whole-cell sugar pattern contained ribose, mannose, arabinose, glucose and galactose. On the basis of phenotypic and genetic data, strains WS4616, WS4617, WS4623 and WS4624 are classified as members of a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is WS4616 ( = DSM 24323 = LMG 26184) and three additional strains are WS4617, WS4623 and WS4624.

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2012-12-01
2019-12-07
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