1887

Abstract

A Gram-negative, facultatively anaerobic, short rod-shaped, heterotrophic bacterium, designated strain YIM D21, was isolated from a salt mine in Yunnan province, south-west China. Strain YIM D21 formed cream–yellow colonies, was non-motile and moderately halophilic, and tolerated NaCl concentrations of 1–15 % (w/v), with optimum growth at 5–10 % (w/v). Growth occurred at 15–42 °C (optimum 28 °C) and at pH 6.5–8.5 (optimum 7.5–8.0). The respiratory quinone was ubiquinone-10 (Q-10). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, three unidentified phospholipids, one unidentified aminolipid and two unidentified lipids. The major fatty acids were Cω7 and cyclo Cω8 and the DNA G+C content was 67.7 mol%. Phylogenetic analyses revealed that strain YIM D21 belongs to the genus . 16S rRNA gene sequence similarities of YIM D21 were 95.7, 95.0 and 94.8 % with the type strains of , and , respectively. Physiological and biochemical tests allowed phenotypic differentiation of strain YIM D21 from closely related species with validly published names. We therefore propose that this isolate represents a novel species, sp. nov.; the type strain is YIM D21 ( = KCTC 23444 = ACCC 10710).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China (NSFC) , (Award 31000003 and 30860013)
  • , Ministry of Science and Technology of China , (Award 2007AA021306 and 863 Program)
  • , Yunnan Provincial Sciences and Technology Department , (Award 2009DA002, 2009CD012 and 2005PY01-1)
  • , Yunnan Provincial Education Department , (Award 2010Y234)
  • , Yunnan University , (Award 2009C14Q and 2010YB002)
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2012-08-01
2021-01-26
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