RT Journal Article SR Electronic(1) A1 Zhao, Guo-Zhen A1 Li, Jie A1 Zhu, Wen-Yong A1 Wei, Da-Qiao A1 Zhang, Jin-Li A1 Xu, Li-Hua A1 Li, Wen-JunYR 2012 T1 Pseudonocardia xishanensis sp. nov., an endophytic actinomycete isolated from the roots of Artemisia annua L. JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_10 SP 2395 OP 2399 DO https://doi.org/10.1099/ijs.0.037028-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, non-motile, endophytic actinomycete, designated strain YIM 63638T, was isolated from the surface-sterilized roots of Artemisia annua L. The isolate grew optimally with 1–3 % (w/v) NaCl, at pH 6.0–7.0 and at 20–37 °C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belonged to the genus Pseudonocardia and showed highest sequence similarity with Pseudonocardia oroxyli D10T (98.9 %), Pseudonocardia ailaonensis YIM 45505T (98.3 %) and Pseudonocardia halophobica IMSNU 21327T (98.0 %). Phylogenetic distance from other type strains of species with validly published names within the genus Pseudonocardia was greater than 2.3 %. Strain YIM 63638T had a genomic DNA G+C content of 72.1 mol% and MK-8(H4) as the predominant respiratory quinone. The major fatty acids were iso-C16 : 0 (36.32 %) and 10-methyl-C16 : 0 (19.78 %). On the basis of phenotypic properties and phylogenetic distinctiveness, strain YIM 63638T represents a novel species of the genus Pseudonocardia , for which the name Pseudonocardia xishanensis sp. nov. is proposed. The type strain is YIM 63638T ( = JCM 17906T  = KCTC 29005T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.037028-0