1887

Abstract

A novel Gram-negative-staining strain, designated 6.2S, was isolated from a soil sample and identified as a biosurfactant producer. Its taxonomic position was investigated using a polyphasic approach. The cells were non-motile, non-spore-forming rods. The organism grew optimally at 30-37 °C, with 0–3 % (w/v) NaCl, and at pH 7.0. Based on 16S rRNA gene sequence analysis, strain 6.2S was found to be a member of the genus and was most closely related to four type species of the genus, showing sequence similarities of 96.8–98.9 %. Partial chaperonin 60 () gene sequence analysis was useful in resolving the phylogenetic relationships between strain 6.2S and closely related taxa, with similarities ranging from 85.5 % (with DSM 11723) to 90.3 % (with CR11 and JCM 21156). The results of DNA–DNA hybridization experiments between the novel strain and its closest relatives gave a DNA–DNA relatedness value of less than 70 %, and consequently confirmed that this new strain did not belong to a previously described species of the genus . The major fatty acids were summed feature 3 (iso-C 2 OH and/or Cω7); iso-C; iso-C 3-OH and C. The G+C content of the genomic DNA was 40.0 mol%. According to its phenotypic and genotypic characteristics and the phylogenetic data, strain 6.2S represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 6.2S ( = CECT 7938 = LMG 26465).

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2012-12-01
2019-08-19
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