1887

Abstract

A taxonomic study was carried out on five Gram-staining-negative, catalase- and oxidase-positive, rod-shaped bacteria isolated from the gills and livers of five diseased rainbow trout. The five novel isolates were designated strains 687B-08, 445-08, 452-08, 453B-08 and 967B-08. In phylogenetic analyses based on 16S rRNA gene sequences, the five novel strains appeared almost identical (99.0–100 % sequence similarity) and to belong to the genus . Strain 687B-08 (the strain selected to represent the five novel isolates) was found to be most closely related to 701B-08 (98.9 % sequence similarity), F-Fue-04IIIaaaa (98.6 %), ATCC 29897 (98.3 %), JS17-8 (98.1 %) and ATCC 35910 (98.1 %). In DNA–DNA hybridizations, DNA–DNA relatedness values of 99–100 % were recorded between the five novel strains. Lower DNA–DNA relatedness values (21–57 %) were recorded between strain 687B-08 and 701B-08, F-Fue-04IIIaaaa and the type strains of other closely related, established species of the genus . The predominant respiratory quinone of strain 687B-08 was MK-6 and the major cellular fatty acids were iso-C, iso-Cω9, iso-C 3-OH and Cω6. The G+C content of the genomic DNA of strain 687B-08 was 38.6 mol%. Based on the phenotypic and genotypic evidence, the five novel strains isolated from rainbow trout represent a single, novel species of the genus , for which the name sp. nov. is proposed. The type strain is 687B-08 ( = CECT 7793  = CCUG 60103).

Funding
This study was supported by the:
  • , Spanish Office for Science and Technology , (Award CENIT 2007-2010)
  • , Spanish Ministry of Science and Innovation , (Award CGL2010-19303)
  • , Junta de Andalucía , (Award P10-CVI-6226)
  • , Västra Götaland Region , (Award -157801, -83080 and VGFOUREG-30781)
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2012-12-01
2020-08-03
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