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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_7

gen. nov., sp. nov., a member of the family isolated from marine mangrove sediment Free

Abstract

A taxonomic study was carried out on strain P1, which was isolated from mangrove sediment samples collected from Qinglan Port (Hainan, China). Cells were curved rods, that were motile, with a single polar flagellum. The strain was non-spore-forming with a cell size of 0.6×1.5–2.2 µm. Catalase and oxidase activities were not detected. Growth was observed in the temperature range 22–44 °C (optimum, 35–40 °C) and pH range 5.5–8.5 (optimum, pH 7.0). NaCl was required for growth and tolerated at up to 3.5 % (w/v) (optimum, 0.5 %). Strain P1 utilized hydrogen, succinate, -malate, citrate, oxalate, -lactate, pyruvate, or cysteine as electron donors, and sulfate or sulfite as electron acceptors. Fermentation products from pyruvate were acetate, H and CO. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain P1 formed a distinct evolutionary lineage within the family . Strain P1 was most closely related to members of the genera (92.0–94.3 % 16S rRNA gene sequence similarity), (91.1 %), (87.9 %) and (86.0 %) of the family . The DNA G+C content of strain P1 was 64.5 mol% and the major cellular fatty acids were iso-C (18.8 %), anteiso-C (5.0 %), C (14.2 %) and iso-Cω9 (24.4 %). The predominant menaquinone was MK-7 (97 %). Major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Strain P1 was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain P1 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is P1 ( = CGMCC 1.5166 = DSM 24233).

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Funding
This study was supported by the:
  • Marine Scientific Research Foundation for Public Sector Program (Award 201105027 and 200805050)
© 2012 IUMS
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2012-07-01
2025-01-17
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Desulfobaculum xiamenensis gen. nov., sp. nov., a member of the family Desulfovibrionaceae isolated from marine mangrove sediment
Int J Syst Evol Microbiol 62, 1570 (2012); https://doi.org/10.1099/ijs.0.036632-0
/content/journal/ijsem/10.1099/ijs.0.036632-0
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