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Volume 62, Issue Pt_9

sp. nov., isolated from seawater Free

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, designated HDW-15, was isolated from seawater of the Yellow Sea, Korea, and subjected to a polyphasic taxonomic study. Strain HDW-15 grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HDW-15 fell within the clade comprising species, joining the type strain of , with which it exhibited highest 16S rRNA gene sequence similarity (97.7 %). The 16S rRNA gene sequence similarity values between strain HDW-15 and the type strains of other species were in the range 93.7–97.2 %. Mean DNA–DNA relatedness values between strain HDW-15 and the type strains of , and were 5.0–9.9 %. The DNA G+C content of the isolate was 48.7 mol%. Strain HDW-15 contained Q-8 as the predominant ubiquinone and Cω7, summed feature 3 (Cω7 and/or iso-C 2-OH) and C as the major fatty acids. The major polar lipids found in strain HDW-15 were phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, showed that strain HDW-15 can be differentiated from other species. On the basis of the data presented, strain HDW-15 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HDW-15 ( = KCTC 23661 = CCUG 61321).

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Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
  • Ministry of Education, Science and Technology (MEST)
© 2012 IUMS
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2012-09-01
2024-04-19
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References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429[PubMed]
     [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  4. Chang H.-W., Roh S. W., Kim K.-H., Nam Y.-D., Yoon J.-H., Oh H.-M., Bae J.-W. 2008; Marinomonas basaltis sp. nov., a marine bacterium isolated from black sand. Int J Syst Evol Microbiol 58:2743–2747 [View Article][PubMed]
     [Google Scholar]
  5. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L. 2011; Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis . Int J Syst Evol Microbiol 61:1170–1175 [View Article][PubMed]
     [Google Scholar]
  6. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [View Article]
     [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  8. Espinosa E., Marco-Noales E., Gómez D., Lucas-Elío P., Ordax M., Garcías-Bonet N., Duarte C. M., Sanchez-Amat A. 2010; Taxonomic study of Marinomonas strains isolated from the seagrass Posidonia oceanica, with descriptions of Marinomonas balearica sp. nov. and Marinomonas pollencensis sp. nov.. Int J Syst Evol Microbiol 60:93–98 [View Article][PubMed]
     [Google Scholar]
  9. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
     [Google Scholar]
  10. Gupta P., Chaturvedi P., Pradhan S., Delille D., Shivaji S. 2006; Marinomonas polaris sp. nov., a psychrohalotolerant strain isolated from coastal sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 56:361–364 [View Article][PubMed]
     [Google Scholar]
  11. Ivanova E. P., Onyshchenko O. M., Christen R., Lysenko A. M., Zhukova N. V., Shevchenko L. S., Kiprianova E. A. 2005; Marinomonas pontica sp. nov., isolated from the Black Sea. Int J Syst Evol Microbiol 55:275–279 [View Article][PubMed]
     [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  13. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  14. Lau K. W. K., Ren J., Wai N. L. M., Lau S. C. L., Qian P.-Y., Wong P.-K., Wu M. 2006; Marinomonas ostreistagni sp. nov., isolated from a pearl-oyster culture pond in Sanya, Hainan Province, China. Int J Syst Evol Microbiol 56:2271–2275 [View Article][PubMed]
     [Google Scholar]
  15. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
     [Google Scholar]
  16. Lucas-Elío P., Marco-Noales E., Espinosa E., Ordax M., López M. M., Garcías-Bonet N., Marbà N., Duarte C. M., Sanchez-Amat A. 2011; Marinomonas alcarazii sp. nov., M. rhizomae sp. nov., M. foliarum sp. nov., M. posidonica sp. nov. and M. aquiplantarum sp. nov., isolated from the microbiota of the seagrass Posidonia oceanica . Int J Syst Evol Microbiol 61:2191–2196 [View Article][PubMed]
     [Google Scholar]
  17. Macián M. C., Arahal D. R., Garay E., Pujalte M. J. 2005; Marinomonas aquamarina sp. nov., isolated from oysters and seawater. Syst Appl Microbiol 28:145–150 [View Article][PubMed]
     [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  19. Prabagaran S. R., Suresh K., Manorama R., Delille D., Shivaji S. 2005; Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica. Int J Syst Evol Microbiol 55:309–313 [View Article][PubMed]
     [Google Scholar]
  20. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T. 2003; Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53:829–832 [View Article][PubMed]
     [Google Scholar]
  21. Romanenko L. A., Tanaka N., Frolova G. M. 2009; Marinomonas arenicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 59:2834–2838 [View Article][PubMed]
     [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  23. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov.. Int J Syst Bacteriol 49:1241–1246 [View Article][PubMed]
     [Google Scholar]
  24. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  25. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
     [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  27. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
     [Google Scholar]
  28. Van Landschoot A., De Ley J. 1984; Marinomonas gen. nov. In Validation of the publication of new names and new combinations previously effectively published outside the IJSB. Validation List no. 13. Int J Syst Bacteriol 34:91–92 [View Article]
     [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
     [Google Scholar]
  31. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
     [Google Scholar]
  32. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
     [Google Scholar]
  33. Yoon J.-H., Kang S.-J., Oh T.-K. 2005; Marinomonas dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55:2303–2307 [View Article][PubMed]
     [Google Scholar]
  34. Zhang D.-C., Li H.-R., Xin Y.-H., Liu H.-C., Chen B., Chi Z.-M., Zhou P.-J., Yu Y. 2008; Marinomonas arctica sp. nov., a psychrotolerant bacterium isolated from the Arctic. Int J Syst Evol Microbiol 58:1715–1718 [View Article][PubMed]
     [Google Scholar]
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Marinomonas hwangdonensis sp. nov., isolated from seawater
Int J Syst Evol Microbiol 62, 2062 (2012); https://doi.org/10.1099/ijs.0.036582-0
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