1887

Abstract

A Gram-staining-positive, filamentous bacterium, which developed large globose sporangia at the ends of long sporangiophores on aerial mycelium, was isolated from dry soil collected in a deciduous forest in Thailand. The cell-wall peptidoglycan of the novel bacterium, which was designated strain A-T 1846, contained -diaminopimelic acid and the whole-cell sugars comprised rhamnose, ribose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H). Mycolic acids were not detected. The identified phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant cellular fatty acids were iso-C, iso-C 2-OH and C 10-methyl. The GC content of the genomic DNA was 72 mol%. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of established members of the genus . Phylogenetic analyses based on 16S rRNA gene sequences also indicated that this strain belonged to the genus but as a representative of a putative novel species. In hybridization experiments, the DNA–DNA relatedness values recorded between the novel strain and established members of the genus all fell well below 70 %. Based on the phenotypic, chemotaxonomic and genotypic evidence and the results of the DNA–DNA hybridizations, strain A-T 1846 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is A-T 1846 ( = BCC 29373  = NBRC 107931). An emended description of the genus is provided.

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2013-01-01
2019-12-13
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