1887

Abstract

Three Gram-reaction-positive bacteria, isolated from the air in a medical practice (strains WS4601, WS4602) or a pharmaceutical clean room (strain WS4599), were characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA and gene sequences of the three novel strains showed that they formed a distinct lineage within the genus , sharing 16S rRNA gene sequence similarities of 96.1–98.0 % with other species of this genus. Chemotaxonomic features also supported the classification of the three novel strains within the genus . The major cellular fatty acids of strain WS4601 were anteiso-C and iso-C, the cell-wall peptidoglycan was of type A3α (-Lys–-Ala), and the predominant respiratory quinones were MK-7(H) and MK-8(H). The polar lipid profile contained diphosphatidylglycerol and phosphatidylglycerol, but no phosphatidylinositol. The G+C content of the genomic DNA was 70.4 mol%. Numerous physiological properties were found that clearly distinguished strains WS4599, WS4601 and WS4602 from established members of the genus . Based on the phenotypic and phylogenetic data, strains WS4599, WS4601 and WS4602 are considered to represent three different strains of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WS4601 ( = DSM 23974 = LMG 26183).

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2013-01-01
2020-05-27
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