1887

Abstract

The taxonomic positions of three streptomycetes isolated from a soil sample from a hay meadow were determined using a polyphasic approach. The isolates had chemical and morphological properties typical of the genus and, in phylogenetic analyses based on 16S rRNA gene sequences, formed a distinct subclade that was most closely related to the subclade. DNA–DNA relatedness studies showed that the novel strains belonged to three different genomic species. The novel strains could be distinguished from one another and from the type strains of the species classified in the subclade using a combination of genotypic and phenotypic properties. On the basis of these data, it is proposed that the novel strains be assigned to the genus as sp. nov., sp. nov. and sp. nov., with BK119 ( = KACC 21001  = CGMCC 4.5797), BK128 ( = KACC 21002  = CGMCC 4.5799) and BK138 ( = KACC 20904  = CGMCC 4.5800) as the respective type strains.

Funding
This study was supported by the:
  • Newcastle University
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 201066/2009-2)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.036384-0
2012-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1908.html?itemId=/content/journal/ijsem/10.1099/ijs.0.036384-0&mimeType=html&fmt=ahah

References

  1. Anderson A. S., Wellington E. M. H. 2001; The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51:797–814 [View Article][PubMed]
    [Google Scholar]
  2. Atalan E., Manfio G. P., Ward A. C., Kroppenstedt R. M., Goodfellow M. 2000; Biosystematic studies on novel streptomycetes from soil. Antonie van Leeuwenhoek 77:337–353 [View Article][PubMed]
    [Google Scholar]
  3. Bentley S. D., Chater K. F., Cerdeño-Tárraga A.-M., Challis G. L., Thomson N. R., James K. D., Harris D. E., Quail M. A., Kieser H. other authors 2002; Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141–147 [View Article][PubMed]
    [Google Scholar]
  4. Collins M. D., Goodfellow M., Minnikin D. E., Alderson G. 1985; Menaquinone composition of mycolic acid-containing actinomycetes and some sporoactinomycetes. J Appl Bacteriol 58:77–86 [View Article][PubMed]
    [Google Scholar]
  5. Euzéby J. P. 2011 List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.cict.fr/
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
    [Google Scholar]
  10. Goodfellow M., Fiedler H. P. 2010; A guide to successful bioprospecting: informed by actinobacterial systematics. Antonie van Leeuwenhoek 98:119–142 [View Article][PubMed]
    [Google Scholar]
  11. Goodfellow M., Kumar Y., Labeda D. P., Sembiring L. 2007; The Streptomyces violaceusniger clade: a home for streptomycetes with rugose ornamented spores. Antonie van Leeuwenhoek 92:173–199 [View Article][PubMed]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
    [Google Scholar]
  13. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J. 2005; Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944 [View Article][PubMed]
    [Google Scholar]
  14. Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. 2003; Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis . Nat Biotechnol 21:526–531 [View Article][PubMed]
    [Google Scholar]
  15. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characterstic. J Bacteriol 57:141–145
    [Google Scholar]
  16. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  17. Kim B. Y., Stach J. E. M., Weon H. Y., Kwon S. W., Goodfellow M. 2010; Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil. Int J Syst Evol Microbiol 60:1813–1823 [View Article][PubMed]
    [Google Scholar]
  18. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp. 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  19. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  20. Küster E., Williams S. T. 1964; Selection of media for isolation of streptomycetes. Nature 202:928–929 [View Article][PubMed]
    [Google Scholar]
  21. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  22. Manfio G., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for description of Streptomyces species. Biotekhnologiya 7:242–283
    [Google Scholar]
  23. Manfio G. P., Atalan E., Zakrzewska-Czerwinska J., Mordarski M., Rodríguez C., Collins M. D., Goodfellow M. 2003; Classification of novel soil streptomycetes as Streptomyces aureus sp. nov., Streptomyces laceyi sp. nov. and Streptomyces sanglieri sp. nov.. Antonie van Leeuwenhoek 83:245–255 [View Article][PubMed]
    [Google Scholar]
  24. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233 [View Article]
    [Google Scholar]
  25. Nagai A., Khan S. T., Tamura T., Takagi M., Shin-ya K. 2011; Streptomyces aomiensis sp. nov., isolated from a soil sample using the membrane-filter method. Int J Syst Evol Microbiol 61:947–950 [View Article][PubMed]
    [Google Scholar]
  26. Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., Yamashita A., Hattori M., Horinouchi S. 2008; Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. J Bacteriol 190:4050–4060 [View Article][PubMed]
    [Google Scholar]
  27. Quintana E. T., Wierzbicka K., Mackiewicz P., Osman A., Fahal A. H., Hamid M. E., Zakrzewska-Czerwinska J., Maldonado L. A., Goodfellow M. 2008; Streptomyces sudanensis sp. nov., a new pathogen isolated from patients with actinomycetoma. Antonie van Leeuwenhoek 93:305–313 [View Article][PubMed]
    [Google Scholar]
  28. Rong X., Huang Y. 2010; Taxonomic evaluation of the Streptomyces griseus clade using multilocus sequence analysis and DNA–DNA hybridization, with proposal to combine 29 species and three subspecies as 11 genomic species. Int J Syst Evol Microbiol 60:696–703 [View Article][PubMed]
    [Google Scholar]
  29. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  31. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  32. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  33. Sun W., Huang Y., Zhang Y.-Q., Liu Z.-H. 2007; Streptomyces emeiensis sp. nov., a novel streptomycete from soil in China. Int J Syst Evol Microbiol 57:1635–1639 [View Article][PubMed]
    [Google Scholar]
  34. Uchida K., Kudo T., Suzuki K. I., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [View Article][PubMed]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  36. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
    [Google Scholar]
  37. Zucchi T. D., Kim B.-Y., Kshetrimayum J. D., Weon H.-Y., Kwon S.-W., Goodfellow M. 2012; Streptomyces brevispora sp. nov. and Streptomyces laculatispora sp. nov., actinomycetes isolated from soil. Int J Syst Evol Microbiol 62:478–483 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.036384-0
Loading
/content/journal/ijsem/10.1099/ijs.0.036384-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error