RT Journal Article SR Electronic(1) A1 Schmidt, Verena S. J. A1 Wenning, Mareike A1 Scherer, SiegfriedYR 2012 T1 Sphingobacterium lactis sp. nov. and Sphingobacterium alimentarium sp. nov., isolated from raw milk and a dairy environment JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_7 SP 1506 OP 1511 DO https://doi.org/10.1099/ijs.0.036327-0 PB Microbiology Society, SN 1466-5034, AB Four non-fermenting, rod-shaped, Gram-staining-negative bacterial strains, designated WCC 4512T, WS 4555, WCC 4521T and WS 4556, were isolated from raw milk and the dairy environment. Phylogenetic analyses based on 16S rRNA and groEL gene sequences demonstrated the affiliation of the four strains to two distinct clusters within the class Sphingobacteriia , phylum ‘ Bacteroidetes ’. Strains WCC 4512T and WS 4555 showed the highest 16S rRNA gene sequence similarity to the type strain of S. daejeonense (97.3 and 97.2 %, respectively), whereas strains WCC 4521T and WS 4556 were most closely related to S. composti LMG 23401T (97.6 % 16S rRNA gene sequence similarity). The DNA G+C contents of strains WCC 4512T and WCC 4521T were 44.2 and 39.3 mol%, respectively. The major cellular fatty acids and the presence of menaquinone MK-7 as the predominant quinone for both strains WCC 4512T and WCC 4521T supported their affiliation to the genus Sphingobacterium . DNA–DNA hybridization experiments between strain WCC 4512T and S. daejeonense LMG 23402T and between strain WCC 4521T and S. composti LMG 23401T revealed DNA relatedness values of 2 % (repetition, 3 %) and 8 % (repetition, 17 %), respectively. On the basis of phenotypic and genetic properties, as well as phylogenetic distinctiveness, it is suggested that the four strains represent two novel Sphingobacterium species with strain WCC 4512T ( = DSM 22361T = LMG 25272T) as the type strain of Sphingobacterium lactis sp. nov. (WS 4555 is a reference strain of S. lactis) and strain WCC 4521T ( = DSM 22362T = LMG 25273T) as the type strain of Sphingobacterium alimentarium sp. nov. (WS 4556 is a reference strain of S. alimentarium)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.036327-0