1887

Abstract

An obligatory anaerobic, moderately halophilic bacterium, designated strain CEJFG43, was isolated from a sample of sediment collected below the salt crust on the hypersaline El Jerid lake, in southern Tunisia. The cells of this novel strain were Gram-staining-negative, non-sporulating, motile, short rods. They grew in media with 6–30 % (w/v) NaCl (optimum 15 %), at 20–60 °C (optimum 45 °C) and at pH 5.5–9.5 (optimum pH 8.3). The micro-organism fermented glucose, fructose, ribose, raffinose, galactose, mannose, sucrose, maltose, xylose, mannitol, pyruvate and glycerol. The products of glucose fermentation were lactate, ethanol, acetate, H and CO. The genomic G+C DNA content of strain CEJFG43 was 33.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CEJFG43 belonged in the genus and was most closely related to DSM 6640 (95.3 % gene sequence similarity) and DSM 9569 (95.3 %). The predominant cellular fatty acids were non-branched (C and C). Based on the phylogenetic and phenotypic evidence, strain CEJFG43 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is CEJFG43 ( = DSM 23230 = JCM 16696).

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2012-08-01
2019-12-08
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