1887

Abstract

A non-motile, coccobacillus-shaped and pink pigmented bacterium, designated strain 03SU10-P, was isolated from wetland freshwater (Woopo wetland, Republic of Korea). Cells were Gram reaction-negative and catalase- and oxidase-positive. The major fatty acids (>10 % of total) were Cω7 and summed feature 3 (iso-C 2-OH and/or Cω7). The predominant respiratory lipoquinone was Q-10. The DNA G+C content was 68 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and an unknown aminolipid. Spermidine, putrescine and 1,3-diaminopropane were the major polyamines. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain 03SU10-P formed an evolutionary lineage within the radiation enclosing the members of the genus . The nearest neighbour to the novel strain was HS-69 (96.3 % gene sequence similarity). The evidence provided by the polyphasic taxonomic approach used in this study indicated that strain 03SU10-P could not be assigned to any recognized species; therefore a novel species is proposed, sp. nov., with 03SU10-P ( = KCTC 23339 = JCM 17520) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.036186-0
2012-12-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/3024.html?itemId=/content/journal/ijsem/10.1099/ijs.0.036186-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M.. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496.[PubMed]
    [Google Scholar]
  3. Benson D. A., Karsch-Mizrachi I., Lipman D. J., Ostell J., Sayers E. W.. ( 2010;). GenBank. . Nucleic Acids Res 38: (Database issue), D46–D51. [CrossRef][PubMed]
    [Google Scholar]
  4. Bibashi E., Sofianou D., Kontopoulou K., Mitsopoulos E., Kokolina E.. ( 2000;). Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. . J Clin Microbiol 38:, 456–457.[PubMed]
    [Google Scholar]
  5. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  6. Christensen W. B.. ( 1946;). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. . J Bacteriol 52:, 461–466.
    [Google Scholar]
  7. Chun J., Goodfellow M.. ( 1995;). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45:, 240–245. [CrossRef][PubMed]
    [Google Scholar]
  8. CLSI ( 2009;). Performance Standards For Antimicrobial Susceptibility Testing, 19th Informational Supplement. CLSI document M100–S19 (ISBN 1–56238–690–5). . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  9. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–288. Edited by Goodfellow M., O’Donnell A. G... London:: Academic Press;.
    [Google Scholar]
  10. Eiler A., Bertilsson S.. ( 2004;). Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. . Environ Microbiol 6:, 1228–1243. [CrossRef][PubMed]
    [Google Scholar]
  11. Embley T. M., Wait R.. ( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O’Donnell A. G... Chichester:: John Wiley & Sons;.
    [Google Scholar]
  12. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  13. Felsenstein J.. ( 1993;). phylip (phylogeny inference package), version 3.5c. Distributed by the author. . Department of Genome Sciences, University of Washington;, Seattle, USA:.
  14. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  15. Furuhata K., Miyamoto H., Goto K., Kato Y., Hara M., Fukuyama M.. ( 2008;). Roseomonas stagni sp. nov., isolated from pond water in Japan. . J Gen Appl Microbiol 54:, 167–171. [CrossRef][PubMed]
    [Google Scholar]
  16. Gallego V., Sánchez-Porro C., García M. T., Ventosa A.. ( 2006;). Roseomonas aquatica sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 56:, 2291–2295. [CrossRef][PubMed]
    [Google Scholar]
  17. Han X. Y., Pham A. S., Tarrand J. J., Rolston K. V., Helsel L. O., Levett P. N.. ( 2003;). Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov.. Am J Clin Pathol 120:, 256–264. [CrossRef][PubMed]
    [Google Scholar]
  18. Jiang C.-Y., Dai X., Wang B.-J., Zhou Y.-G., Liu S.-J.. ( 2006;). Roseomonas lacus sp. nov., isolated from freshwater lake sediment. . Int J Syst Evol Microbiol 56:, 25–28. [CrossRef][PubMed]
    [Google Scholar]
  19. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  20. Kim M. S., Baik K. S., Park S. C., Rhee M. S., Oh H.-M., Seong C. N.. ( 2009;). Roseomonas frigidaquae sp. nov., isolated from a water-cooling system. . Int J Syst Evol Microbiol 59:, 1630–1634. [CrossRef][PubMed]
    [Google Scholar]
  21. Kovacs N.. ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703. [CrossRef][PubMed]
    [Google Scholar]
  22. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  23. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  25. Powers E. M.. ( 1995;). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  26. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L.. ( 1993;). Roseomonas, a new genus associated with bacteremia and other human infections. . J Clin Microbiol 31:, 3275–3283.[PubMed]
    [Google Scholar]
  27. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Sandoe J. A. T., Malnick H., Loudon K. W.. ( 1997;). A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. . J Clin Microbiol 35:, 2150–2152.[PubMed]
    [Google Scholar]
  29. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  30. Scherer P., Kneifel H.. ( 1983;). Distribution of polyamines in methanogenic bacteria. . J Bacteriol 154:, 1315–1322.[PubMed]
    [Google Scholar]
  31. September S. M., Brözel V. S., Venter S. N.. ( 2004;). Diversity of nontuberculoid Mycobacterium species in biofilms of urban and semiurban drinking water distribution systems. . Appl Environ Microbiol 70:, 7571–7573. [CrossRef][PubMed]
    [Google Scholar]
  32. Sfanos K., Harmody D., Dang P., Ledger A., Pomponi S., McCarthy P., Lopez J.. ( 2005;). A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates. . Syst Appl Microbiol 28:, 242–264. [CrossRef][PubMed]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  34. Staley J. T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  35. Subudhi C. P. K., Adedeji A., Kaufmann M. E., Lucas G. S., Kerr J. R.. ( 2001;). Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. . Clin Microbiol Infect 7:, 573–575. [CrossRef][PubMed]
    [Google Scholar]
  36. Swofford D. L.. ( 1998;). Phylogenetic analysis using parsimony (paup), version 4. . Sunderland, MA:: Sinauer Associates;.
  37. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  38. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  39. Yamaguchi S., Yokoe M.. ( 2000;). A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. . Appl Environ Microbiol 66:, 3337–3343. [CrossRef][PubMed]
    [Google Scholar]
  40. Yoon J. H., Kang S. J., Oh H. W., Oh T. K.. ( 2007;). Roseomonas terrae sp. nov.. Int J Syst Evol Microbiol 57:, 2485–2488. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.036186-0
Loading
/content/journal/ijsem/10.1099/ijs.0.036186-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error