1887

Abstract

A non-motile, coccobacillus-shaped and pink pigmented bacterium, designated strain 03SU10-P, was isolated from wetland freshwater (Woopo wetland, Republic of Korea). Cells were Gram reaction-negative and catalase- and oxidase-positive. The major fatty acids (>10 % of total) were Cω7 and summed feature 3 (iso-C 2-OH and/or Cω7). The predominant respiratory lipoquinone was Q-10. The DNA G+C content was 68 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and an unknown aminolipid. Spermidine, putrescine and 1,3-diaminopropane were the major polyamines. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain 03SU10-P formed an evolutionary lineage within the radiation enclosing the members of the genus . The nearest neighbour to the novel strain was HS-69 (96.3 % gene sequence similarity). The evidence provided by the polyphasic taxonomic approach used in this study indicated that strain 03SU10-P could not be assigned to any recognized species; therefore a novel species is proposed, sp. nov., with 03SU10-P ( = KCTC 23339 = JCM 17520) as the type strain.

Funding
This study was supported by the:
  • Basic Science Research Program (Award 2010-0006861)
  • National Research Foundation of Korea (NRF)
  • 21C Frontier Microbial Genomics and Applications Center Program
  • Ministry of Education, Science and Technology
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment, Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.036186-0
2012-12-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/3024.html?itemId=/content/journal/ijsem/10.1099/ijs.0.036186-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  2. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
    [Google Scholar]
  3. Benson D. A., Karsch-Mizrachi I., Lipman D. J., Ostell J., Sayers E. W. 2010; GenBank. Nucleic Acids Res 38:Database issueD46–D51 [View Article][PubMed]
    [Google Scholar]
  4. Bibashi E., Sofianou D., Kontopoulou K., Mitsopoulos E., Kokolina E. 2000; Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 38:456–457[PubMed]
    [Google Scholar]
  5. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  6. Christensen W. B. 1946; Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52:461–466
    [Google Scholar]
  7. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  8. CLSI 2009; Performance Standards For Antimicrobial Susceptibility Testing, 19th Informational Supplement. CLSI document M100–S19 (ISBN 1–56238–690–5). Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  9. Collins M. D. 1985; Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–288 Edited by Goodfellow M., O’Donnell A. G. London: Academic Press;
    [Google Scholar]
  10. Eiler A., Bertilsson S. 2004; Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environ Microbiol 6:1228–1243 [View Article][PubMed]
    [Google Scholar]
  11. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by Goodfellow M., O’Donnell A. G. Chichester: John Wiley & Sons;
    [Google Scholar]
  12. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  13. Felsenstein J. 1993; phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington; Seattle, USA:
    [Google Scholar]
  14. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  15. Furuhata K., Miyamoto H., Goto K., Kato Y., Hara M., Fukuyama M. 2008; Roseomonas stagni sp. nov., isolated from pond water in Japan. J Gen Appl Microbiol 54:167–171 [View Article][PubMed]
    [Google Scholar]
  16. Gallego V., Sánchez-Porro C., García M. T., Ventosa A. 2006; Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56:2291–2295 [View Article][PubMed]
    [Google Scholar]
  17. Han X. Y., Pham A. S., Tarrand J. J., Rolston K. V., Helsel L. O., Levett P. N. 2003; Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov.. Am J Clin Pathol 120:256–264 [View Article][PubMed]
    [Google Scholar]
  18. Jiang C.-Y., Dai X., Wang B.-J., Zhou Y.-G., Liu S.-J. 2006; Roseomonas lacus sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:25–28 [View Article][PubMed]
    [Google Scholar]
  19. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  20. Kim M. S., Baik K. S., Park S. C., Rhee M. S., Oh H.-M., Seong C. N. 2009; Roseomonas frigidaquae sp. nov., isolated from a water-cooling system. Int J Syst Evol Microbiol 59:1630–1634 [View Article][PubMed]
    [Google Scholar]
  21. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
    [Google Scholar]
  22. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  23. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  25. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758[PubMed]
    [Google Scholar]
  26. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L. 1993; Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 31:3275–3283[PubMed]
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  28. Sandoe J. A. T., Malnick H., Loudon K. W. 1997; A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 35:2150–2152[PubMed]
    [Google Scholar]
  29. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  30. Scherer P., Kneifel H. 1983; Distribution of polyamines in methanogenic bacteria. J Bacteriol 154:1315–1322[PubMed]
    [Google Scholar]
  31. September S. M., Brözel V. S., Venter S. N. 2004; Diversity of nontuberculoid Mycobacterium species in biofilms of urban and semiurban drinking water distribution systems. Appl Environ Microbiol 70:7571–7573 [View Article][PubMed]
    [Google Scholar]
  32. Sfanos K., Harmody D., Dang P., Ledger A., Pomponi S., McCarthy P., Lopez J. 2005; A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates. Syst Appl Microbiol 28:242–264 [View Article][PubMed]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  34. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
    [Google Scholar]
  35. Subudhi C. P. K., Adedeji A., Kaufmann M. E., Lucas G. S., Kerr J. R. 2001; Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. Clin Microbiol Infect 7:573–575 [View Article][PubMed]
    [Google Scholar]
  36. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  37. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  38. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  39. Yamaguchi S., Yokoe M. 2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343 [View Article][PubMed]
    [Google Scholar]
  40. Yoon J. H., Kang S. J., Oh H. W., Oh T. K. 2007; Roseomonas terrae sp. nov.. Int J Syst Evol Microbiol 57:2485–2488 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.036186-0
Loading
/content/journal/ijsem/10.1099/ijs.0.036186-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error