1887

Abstract

A Gram-type positive, Gram-reaction variable, non-motile, psychrophilic actinobacterium, designated Cr8-25, was isolated from alpine glacier cryoconite and was able to grow well over a temperature range of 1–15 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Cr8-25 belonged to the family and showed highest 16S rRNA gene sequence similarity with 44C3 (97.0 %). However, strain Cr8-25 could be differentiated from the type strain of on the level of genomospecies by a DNA–DNA relatedness value of only 37.2 %. Strain Cr8-25 contained a cell-wall peptidoglycan that was cross-linked according to the B-type, which is based on 2,4-diaminobutyric acid. The cell wall contained the sugars galactose, fucose and rhamnose. The predominant cellular fatty acids of strain Cr8-25 were C anteiso (64.6 %) and iso-C (22.5 %) and the major menaquinones were MK-11 and MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and unknown glycolipids. The G+C content of the genomic DNA was 58.8 mol%. On the basis of the phenotypic characteristics, phylogenetic and chemotaxonomic analyses and DNA–DNA relatedness data, strain Cr8-25 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is Cr8-25 ( = DSM 23737 = LMG 26215).

Funding
This study was supported by the:
  • Aktion D. Swarovski & Co. 2009
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2012-11-01
2022-01-22
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