RT Journal Article SR Electronic(1) A1 Sazak, Anil A1 Camas, Mustafa A1 Spröer, Cathrin A1 Klenk, Hans-Peter A1 Sahin, NevzatYR 2012 T1 Actinomadura geliboluensis sp. nov., isolated from soil JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_8 SP 2011 OP 2017 DO https://doi.org/10.1099/ijs.0.036145-0 PB Microbiology Society, SN 1466-5034, AB A novel actinobacterium, strain A8036T, isolated from soil, was investigated by using a polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generated spiral chains of spores with irregular surfaces. The cell wall contained meso-diaminopimelic acid (type III) and cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9(H6) and MK-9(H4). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C16 : 0, C17 : 1 cis9, C16 : 0, C15 : 0 and 10-methyl C17 : 0. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain A8036T were Actinomadura meyerae DSM 44715T (99.23 % similarity), Actinomadura bangladeshensis DSM 45347T (98.9 %) and Actinomadura chokoriensis DSM 45346T (98.3 %). However, DNA–DNA relatedness and phenotypic data demonstrated that strain A8036T could be clearly distinguished from the type strains of all closely related Actinomadura species. Strain A8036T is therefore considered to represent a novel species of the genus Actinomadura , for which the name Actinomadura geliboluensis sp. nov. is proposed. The type strain is A8036T ( = DSM 45508T = KCTC 19868T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.036145-0