1887

Abstract

A novel actinobacterium, strain A8036, isolated from soil, was investigated by using a polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generated spiral chains of spores with irregular surfaces. The cell wall contained -diaminopimelic acid (type III) and cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9(H) and MK-9(H). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C, C 9, C, C and 10-methyl C. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain A8036 were DSM 44715 (99.23 % similarity), DSM 45347 (98.9 %) and DSM 45346 (98.3 %). However, DNA–DNA relatedness and phenotypic data demonstrated that strain A8036 could be clearly distinguished from the type strains of all closely related species. Strain A8036 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A8036 ( = DSM 45508 = KCTC 19868).

Funding
This study was supported by the:
  • Ondokuz Mayis University (OMU) (Award PYO. FEN. 1901.09.003)
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2012-08-01
2022-01-18
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