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Volume 62, Issue Pt_8

gen. nov., sp. nov., a member of the family isolated from mangrove sediment Free

Abstract

A Gram-negative, obligately anaerobic, non-spore-forming, long rod-shaped bacterium strain P2 was isolated from the offshore mangrove sediment of the South China Sea. Growth was observed at between 22 and 39 °C, with an optimum at 35 °C. The pH range for growth was 5.0–8.5, with an optimum around pH 7.0–7.5. Salt tolerance was determined between 0.2 and 3.5 % (w/v), optimum at 0.5–1.0 %. Catalase and oxidase activities were negative. Strain P2 utilized cysteine, lactate, pyruvate, yeast extract or H/CO+acetate as electron donors, and sulfate or sulfite as electron acceptors. Metabolism was strictly fermentative. The main organic fermentation products were propionate, acetate and succinate. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain P2 formed a distinct evolutionary lineage within the family . Strain P2 was most closely related to members of the genera (92.0 % 16S rRNA gene sequence similarity), (91.7 %) and (89.9 %) of the family . The DNA G+C content of the novel strain was 44.2±1.0 mol%. The dominant fatty acids of strain P2 were iso-C (33.5 %), anteiso-C (18.9 %), C (5.4 %), C 3-OH (7.7 %) and iso-C 3-OH (13.3 %). The respiratory quinone was menaquinone 7 (100 % of total quinone) and the major polar lipid was phosphatidylethanolamine. Strain P2 was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of phylogenetic, phenotypic and physiological evidence, a novel genus, , is proposed to harbour strain P2 ( = CGMCC 1.5167 = DSM 24214) which is described as the type strain of a novel species, gen. nov., sp. nov.

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Funding
This study was supported by the:
  • Marine Scientific Research Foundation for Pubic Sector Program (Award 201105027 and 200805050)
© 2012 IUMS
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2012-08-01
2024-12-06
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Mangroviflexus xiamenensis gen. nov., sp. nov., a member of the family Marinilabiliaceae isolated from mangrove sediment
Int J Syst Evol Microbiol 62, 1819 (2012); https://doi.org/10.1099/ijs.0.036137-0
/content/journal/ijsem/10.1099/ijs.0.036137-0
/content/journal/ijsem/10.1099/ijs.0.036137-0
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