1887

Abstract

A salmon-red-pigmented bacterial strain, designated M-8, was isolated from a polluted farmland soil sample in China and was characterized in a taxonomic study using a polyphasic approach. Strain M-8 was Gram-stain-negative, rod-shaped, non-motile and non-spore-forming. Growth occurred at 20–37 °C, at pH 5.0–10.0 and with 0–2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-8 belonged to the genus .16S rRNA gene sequence similarity values between strain M-8 and the type strains of the three recognized species of the genus , KACC 11310, LMG 24825 and KACC 13047, were 97.1, 96.3 and 95.3 %, respectively. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major fatty acids were iso-C, iso-C 3-OH and summed feature 3 (comprising Cω7 and/or Cω6). The DNA G+C content of strain M-8 was 47.0 mol%. On the basis of genotypic and phenotypic data, strain M-8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-8 ( = CCTCC AB 2010401 = KCTC 23299). An emended description of the genus is also presented.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31000001 and 30900044)
  • Fund for the Doctoral Program of Higher Education (Award 20090097120031)
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2012-11-01
2021-10-28
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References

  1. Beveridge T. J., Lawrence J. R., Murray R. G. E. 2007; Sampling and staining for light microscopy. In Methods for General and Molecular Microbiology, 3rd edn. pp. 19–33 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder R. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T. 1995; Purification and properties of extracellular amylase from the hyperthermophilic archaeon Thermococccus profundus DT5432. Appl Environ Microbiol 61:1502–1506[PubMed]
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  5. Fautz E., Reichenbach H. 1980; A simple test for flexirubin type pigments. FEMS Microbiol Lett 8:87–91 [View Article]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  9. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  10. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  13. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
    [Google Scholar]
  14. Sheu S. Y., Cho N. T., Arun A. B., Chen W. M. 2010; Terrimonas aquatica sp. nov., isolated from a freshwater spring. Int J Syst Evol Microbiol 60:2705–2709 [View Article][PubMed]
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  18. Xie C. H., Yokota A. 2006; Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1117–1121 [View Article][PubMed]
    [Google Scholar]
  19. Zhou Y., Dong J., Wang X., Huang X., Zhang K. Y., Zhang Y. Q., Guo Y. F., Lai R., Li W. J. 2007; Chryseobacterium flavum sp. nov., isolated from polluted soil. Int J Syst Evol Microbiol 57:1765–1769 [View Article][PubMed]
    [Google Scholar]
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