1887

Abstract

The bacterial strain LH2-2 was isolated from freshwater of Longhu Lake, a slightly alkaline lake (pH 8.8) in north-east China. Cells of strain LH2-2 were Gram-staining-negative, non-spore-forming rods, 0.3–0.5 µm wide and 2.0–4.0 µm long. Cells were motile by means of a single polar flagellum. The strain was strictly aerobic and heterotrophic and oxidase- and catalase-positive. Growth occurred at 0–36 °C (optimum, 26–34 °C), pH 6.5–11 (optimum, pH 8.0–8.6) and in the presence of 0–2 % (w/v) NaCl (optimum, 1 %). Strain LH2-2 contained Q-8 as the major respiratory quinone. The major fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH; 21.9 %), Cω8 (18.9 %), Cω7 (16.4 %) and C (12.7 %) after growth on marine agar 2216. The DNA G+C content was 47 mol% ( ). The 16S rRNA gene and a conserved portion of the gene were sequenced and used for phylogenetic analyses. Phylogenetic trees based on 16S rRNA gene and sequences showed that strain LH2-2 was associated with the genus and closely related to the type strains of species, and showed the highest 16S rRNA gene sequence similarity to KMM 1406 (97.4 %), SW-353 (97.1 %) and K19414 (96.5 %). The DNA–DNA relatedness of strain LH2-2 to NBRC 103167, JCM 14331 and LMG 23818 was 39, 31 and 23 %, respectively. Based on these results, it is concluded that strain LH2-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LH2-2 ( = CGMCC 1.7003  = NBRC 105632). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • Hi-Tech Research and Development Program of China ‘863’ program (Award 2006AA06Z316)
  • Knowledge Innovation Program of the Chinese Academy of Sciences (Award KSCS2-YW-G-055-01)
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2012-12-01
2021-10-15
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