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Abstract

The bacterial strain LH2-2 was isolated from freshwater of Longhu Lake, a slightly alkaline lake (pH 8.8) in north-east China. Cells of strain LH2-2 were Gram-staining-negative, non-spore-forming rods, 0.3–0.5 µm wide and 2.0–4.0 µm long. Cells were motile by means of a single polar flagellum. The strain was strictly aerobic and heterotrophic and oxidase- and catalase-positive. Growth occurred at 0–36 °C (optimum, 26–34 °C), pH 6.5–11 (optimum, pH 8.0–8.6) and in the presence of 0–2 % (w/v) NaCl (optimum, 1 %). Strain LH2-2 contained Q-8 as the major respiratory quinone. The major fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH; 21.9 %), Cω8 (18.9 %), Cω7 (16.4 %) and C (12.7 %) after growth on marine agar 2216. The DNA G+C content was 47 mol% ( ). The 16S rRNA gene and a conserved portion of the gene were sequenced and used for phylogenetic analyses. Phylogenetic trees based on 16S rRNA gene and sequences showed that strain LH2-2 was associated with the genus and closely related to the type strains of species, and showed the highest 16S rRNA gene sequence similarity to KMM 1406 (97.4 %), SW-353 (97.1 %) and K19414 (96.5 %). The DNA–DNA relatedness of strain LH2-2 to NBRC 103167, JCM 14331 and LMG 23818 was 39, 31 and 23 %, respectively. Based on these results, it is concluded that strain LH2-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LH2-2 ( = CGMCC 1.7003  = NBRC 105632). An emended description of the genus is also provided.

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2012-12-01
2020-01-23
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Bianchi A. , Bianchi M. . ( 1995; ). Bacterial diversity and ecosystem maintenance: an overview. . In Microbial Diversity and Ecosystem Maintenance, pp. 185–198. Edited by Hawksworth D. L. , Colwell R. R. . . Wallingford, UK:: CAB International;.
    [Google Scholar]
  3. Brettar I. , Christen R. , Höfle M. G. . ( 2002; ). Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. . Int J Syst Evol Microbiol 52:, 1851–1857. [CrossRef] [PubMed]
    [Google Scholar]
  4. Brettar I. , Christen R. , Höfle M. G. . ( 2006; ). Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. . Int J Syst Evol Microbiol 56:, 2177–2183. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chen W. M. , Lin C. Y. , Young C. C. , Sheu S. Y. . ( 2010; ). Rheinheimera aquatica sp. nov., an antimicrobial activity producing bacterium isolated from freshwater culture pond. . J Microbiol Biotechnol 20:, 1386–1392. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  7. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  8. Dong X.-Z. , Cai M.-Y. . ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation)..
  9. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  10. Fitch W. M. . ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  11. Fonnesbech Vogel B. , Venkateswaran K. , Christensen H. , Falsen E. , Christiansen G. , Gram L. . ( 2000; ). Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. . Int J Syst Evol Microbiol 50:, 1133–1142. [CrossRef] [PubMed]
    [Google Scholar]
  12. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  13. Halpern M. , Senderovich Y. , Snir S. . ( 2007; ). Rheinheimera chironomi sp. nov., isolated from a chironomid (Diptera; Chironomidae) egg mass. . Int J Syst Evol Microbiol 57:, 1872–1875. [CrossRef] [PubMed]
    [Google Scholar]
  14. Hu Y.-T. , Zhou P.-J. , Zhou Y.-G. , Liu Z.-H. , Liu S.-J. . ( 2004; ). Saccharothrix xinjiangensis sp. nov., a pyrene-degrading actinomycete isolated from Tianchi Lake, Xinjiang, China. . Int J Syst Evol Microbiol 54:, 2091–2094. [CrossRef] [PubMed]
    [Google Scholar]
  15. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  16. Kates M. . ( 1986; ). Techniques of Lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  17. Kim M. S. , Roh S. W. , Nam Y. D. , Chang H. W. , Kim K. H. , Jung M. J. , Choi J. H. , Park E. J. , Bae J. W. . ( 2009; ). Alishewanella jeotgali sp. nov., isolated from traditional fermented food, and emended description of the genus Alishewanella . . Int J Syst Evol Microbiol 59:, 2313–2316. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kim M. S. , Jo S. K. , Roh S. W. , Bae J. W. . ( 2010; ). Alishewanella agri sp. nov., isolated from landfill soil. . Int J Syst Evol Microbiol 60:, 2199–2203. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  20. Li H. J. , Zhang X. Y. , Zhang Y. J. , Zhou M. Y. , Gao Z. M. , Chen X. L. , Dang H. Y. , Zhang Y. Z. . ( 2011; ). Rheinheimera nanhaiensis sp. nov., isolated from marine sediments, and emended description of the genus Rheinheimera Brettar et al. 2002 emend. Merchant et al. 2007. . Int J Syst Evol Microbiol 61:, 1016–1022. [CrossRef] [PubMed]
    [Google Scholar]
  21. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  22. Merchant M. M. , Welsh A. K. , McLean R. J. . ( 2007; ). Rheinheimera texasensis sp. nov., a halointolerant freshwater oligotroph. . Int J Syst Evol Microbiol 57:, 2376–2380. [CrossRef] [PubMed]
    [Google Scholar]
  23. Pinhassi J. , Berman T. . ( 2003; ). Differential growth response of colony-forming α- and γ-proteobacteria in dilution culture and nutrient addition experiments from Lake Kinneret (Israel), the eastern Mediterranean Sea, and the Gulf of Eilat. . Appl Environ Microbiol 69:, 199–211. [CrossRef] [PubMed]
    [Google Scholar]
  24. Poretsky R. S. , Bano N. , Buchan A. , LeCleir G. , Kleikemper J. , Pickering M. , Pate W. M. , Moran M. A. , Hollibaugh J. T. . ( 2005; ). Analysis of microbial gene transcripts in environmental samples. . Appl Environ Microbiol 71:, 4121–4126. [CrossRef] [PubMed]
    [Google Scholar]
  25. Roh S. W. , Nam Y. D. , Chang H. W. , Kim K. H. , Kim M. S. , Oh H. M. , Bae J. W. . ( 2009; ). Alishewanella aestuarii sp. nov., isolated from tidal flat sediment, and emended description of the genus Alishewanella . . Int J Syst Evol Microbiol 59:, 421–424. [CrossRef] [PubMed]
    [Google Scholar]
  26. Romanenko L. A. , Uchino M. , Falsen E. , Zhukova N. V. , Mikhailov V. V. , Uchimura T. . ( 2003; ). Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. . Int J Syst Evol Microbiol 53:, 1973–1977. [CrossRef] [PubMed]
    [Google Scholar]
  27. Ryu S. H. , Chung B. S. , Park M. , Lee S. S. , Lee S. S. , Jeon C. O. . ( 2008; ). Rheinheimera soli sp. nov., a gammaproteobacterium isolated from soil in Korea. . Int J Syst Evol Microbiol 58:, 2271–2274. [CrossRef] [PubMed]
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  30. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  31. Yamamoto S. , Harayama S. . ( 1995; ). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. . Appl Environ Microbiol 61:, 1104–1109.[PubMed]
    [Google Scholar]
  32. Yoon J.-H. , Park S. E. , Kang S.-J. , Oh T.-K. . ( 2007; ). Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol 57:, 1386–1390. [CrossRef] [PubMed]
    [Google Scholar]
  33. Zhang D. , Yang H. , Zhang W. , Huang Z. , Liu S.-J. . ( 2003; ). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. . Int J Syst Evol Microbiol 53:, 1111–1114. [CrossRef] [PubMed]
    [Google Scholar]
  34. Zhang X. X. , Sun L. , Qiu F. B. , McLean R. J. C. , Jiang R. B. , Song W. . ( 2008; ). Rheinheimera tangshanensis sp. nov., a rice root-associated bacterium. . Int J Syst Evol Microbiol 58:, 2420–2424. [CrossRef] [PubMed]
    [Google Scholar]
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