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Volume 62, Issue Pt_9

sp. nov., isolated from marine sediment Free

Abstract

A novel actinomycete, strain S7F-02, which produced primary branched hyphae and fragmented into V- and Y-shaped bacillary cells, was isolated from marine sediment collected in the Andaman Sea, Trang Province, Thailand. Lysine was found to be the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars of strain S7F-02 were ribose, arabinose and glucose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H). The predominant cellular fatty acids were anteiso-C and iso-C. The G+C content of the genomic DNA of strain S7F-02 was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S7F-02 should be classified in the genus . This strain formed a close association with DSM 43110, with which it shared 99.2 % 16S rRNA gene sequence similarity. DNA–DNA hybridization data together with physiological and biochemical properties showed that strain S7F-02 could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain S7F-02 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S7F-02 ( = BCC 41922 = JCM 17130).

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Funding
This study was supported by the:
  • King Mongkut’s Institute of Technology Ladkrabang Research Fund (KReF)
© 2012 IUMS
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2012-09-01
2024-04-19
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References

  1. Alonso-Vega P., Santamaría R. I., Martínez-Molina E., Trujillo M. E. 2008; Promicromonospora kroppenstedtii sp. nov., isolated from sandy soil. Int J Syst Evol Microbiol 58:1476–1481 [View Article][PubMed]
     [Google Scholar]
  2. Arai T. 1975 Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
     [Google Scholar]
  3. Busse H.-J., Zlamala C., Buczolits S., Lubitz W., Kämpfer P., Takeuchi M. 2003; Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 53:1503–1507 [View Article][PubMed]
     [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
     [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
     [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
     [Google Scholar]
  9. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [View Article]
     [Google Scholar]
  10. Jiang Y., Wiese J., Cao Y. R., Xu L.-H., Imhoff J. F., Jiang C. L. 2009; Promicromonospora flava sp. nov., isolated from sediment of the Baltic Sea. Int J Syst Evol Microbiol 59:1599–1602 [View Article][PubMed]
     [Google Scholar]
  11. Kaewkla O., Franco C. M. M. 2012; Promicromonospora endophytica sp. nov., an endophytic actinobacterium isolated from the root of an Australian native Grey Box tree. Int J Syst Evol Microbiol 62:1687–1691 [View Article][PubMed]
     [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
     [Google Scholar]
  13. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146:527–534[PubMed]
     [Google Scholar]
  14. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standard Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
     [Google Scholar]
  15. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
     [Google Scholar]
  16. Komagata K., Suzuki K. I. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  17. Krasil’nikov N. A., Kalakoutskii L. V., Kirillova N. F. 1961; A new genus of Actinomycetales, Promicromonospora gen. nov.. Bull Acad Sci USSR Ser Biol 1:107–112
     [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp. 227–291 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
     [Google Scholar]
  19. Martin K., Schäfer J., Kämpfer P. 2010; Promicromonospora umidemergens sp. nov., isolated from moisture from indoor wall material. Int J Syst Evol Microbiol 60:537–541 [View Article][PubMed]
     [Google Scholar]
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  21. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [View Article][PubMed]
     [Google Scholar]
  22. Qin S., Jiang J. H., Klenk H. P., Zhu W. Y., Zhao G. Z., Zhao L. X., Tang S. K., Xu L. H., Li W. J. 2012; Promicromonospora xylanilytica sp. nov., an endophytic actinomycete isolated from surface-sterilized leaves of the medicinal plant Maytenus austroyunnanensis . Int J Syst Evol Microbiol 62:84–89 [View Article][PubMed]
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  24. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  26. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:479–491 [View Article]
     [Google Scholar]
  27. Takahashi Y., Tanaka Y., Iwai Y., Ōmura S. 1987; Promicromonospora sukumoe sp. nov., a new species of the Actinomycetales . J Gen Appl Microbiol 33:507–519 [View Article]
     [Google Scholar]
  28. Tamaoka J. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp. 463–470 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  29. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  31. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Suzuki K.-I., Kudo T. 2005; Micromonospora eburnea sp. nov., isolated from a Thai peat swamp forest. Int J Syst Evol Microbiol 55:417–422 [View Article][PubMed]
     [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  34. Verlander C. P. 1992; Detection of horseradish peroxidase by colorimetry. In Nonisotopic DNA Probe Techniques pp. 185–201 Edited by Kricka L. J. New York: Academic Press; [CrossRef]
     [Google Scholar]
  35. Williams S. T., Cross T. 1971; Actinomycetes. In Methods in Microbiology vol. 4 pp. 295–334 Edited by Booth C. London: Academic Press;
     [Google Scholar]
  36. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
     [Google Scholar]
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Promicromonospora thailandica sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 62, 2140 (2012); https://doi.org/10.1099/ijs.0.035972-0
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