1887

Abstract

An aerobic, Gram-negative-staining, motile, psychrophilic bacterium, designated strain S8-3, was isolated from alpine soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S8-3 was related to the genus and had highest 16S rRNA gene sequence similarity with S213 (98.0 %). 16S RNA gene sequence similarity between strain S8-3 and ATCC 29837 (the type species of the genus ) was 93.0 %. Strain S8-3 contained Q-10 as the ubiquinone and Cω7 (65.0 %) and C 2-OH (13.4 %) as the dominant fatty acids (>10 %). The major polyamines were the triamine homospermidine and spermidine. The polar lipid profile contained sphingoglycolipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The DNA G+C content was 64.1 mol%. Combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain S8-3 is a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S8-3 ( = DSM 22537 = LMG 26055).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035964-0
2012-07-01
2024-10-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1558.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035964-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [View Article][PubMed]
    [Google Scholar]
  2. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  3. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [View Article]
    [Google Scholar]
  4. Busse H. J., Kämpfer P., Denner E. B. 1999; Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23:242–251 [View Article][PubMed]
    [Google Scholar]
  5. Busse H.-J., Denner E. B. M., Buczolits S., Salkinoja-Salonen M., Bennasar A., Kämpfer P. 2003; Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas . Int J Syst Evol Microbiol 53:1253–1260 [View Article][PubMed]
    [Google Scholar]
  6. Busse H.-J., Hauser E., Kämpfer P. 2005; Description of two novel species, Sphingomonas abaci sp. nov. and Sphingomonas panni sp. nov.. Int J Syst Evol Microbiol 55:2565–2569 [View Article][PubMed]
    [Google Scholar]
  7. Cavicchioli R. 2006; Cold-adapted archaea. Nat Rev Microbiol 4:331–343 [View Article][PubMed]
    [Google Scholar]
  8. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  9. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  10. Denner E. B. M., Kämpfer P., Busse H.-J., Moore E. R. B. 1999; Reclassification of Pseudomonas echinoides Heumann 1962, 343AL, in the genus Sphingomonas as Sphingomonas echinoides comb. nov.. Int J Syst Bacteriol 49:1103–1109 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Geueke B., Busse H.-J., Fleischmann T., Kämpfer P., Kohler H.-P. E. 2007; Description of Sphingosinicella xenopeptidilytica sp. nov., a β-peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans . Int J Syst Evol Microbiol 57:107–113 [View Article][PubMed]
    [Google Scholar]
  13. Huß V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article]
    [Google Scholar]
  14. Kämpfer P., Meurer U., Esser M., Hirsch T., Busse H.-J. 2007; Sphingomonas pseudosanguinis sp. nov., isolated from the water reservoir of an air humidifier. Int J Syst Evol Microbiol 57:1342–1345 [View Article][PubMed]
    [Google Scholar]
  15. Margesin R. 2009; Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. Extremophiles 13:257–262 [View Article][PubMed]
    [Google Scholar]
  16. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F. 2003; Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7:451–458 [View Article][PubMed]
    [Google Scholar]
  17. Margesin R., Schinner F., Marx J. C., Gerday C. (editors) 2008 Psychrophiles: from Biodiversity to Biotechnology Berlin, Heidelberg: Springer; [View Article]
    [Google Scholar]
  18. Margesin R., Jud M., Tscherko D., Schinner F. 2009; Microbial communities and activities in alpine and subalpine soils. FEMS Microbiol Ecol 67:208–218 [View Article][PubMed]
    [Google Scholar]
  19. Margesin R., Schumann P., Zhang D. C., Redzic M., Zhou Y. G., Liu H. C., Schinner F. 2012; Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 62:397–402 [View Article][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  21. Morita R. Y. 1975; Psychrophilic bacteria. Bacteriol Rev 39:144–167[PubMed]
    [Google Scholar]
  22. Nigam A., Jit S., Lal R. 2010; Sphingomonas histidinilytica sp. nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 60:1038–1043 [View Article][PubMed]
    [Google Scholar]
  23. Ohta H., Hattori R., Ushiba Y., Mitsui H., Ito M., Watanabe H., Tonosaki A., Hattori T. 2004; Sphingomonas oligophenolica sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations. Int J Syst Evol Microbiol 54:2185–2190 [View Article][PubMed]
    [Google Scholar]
  24. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7[PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  26. Sambrook J., Frisch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  28. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:572–576 [View Article][PubMed]
    [Google Scholar]
  29. Süßmuth R., Eberspächer J., Haag R., Springer W. 1987 Biochemisch-mikrobiologisches Praktikum Stuttgart: Georg Thieme Verlag;
    [Google Scholar]
  30. Takeuchi M., Kawai F., Shimada Y., Yokota A. 1993; Taxonomic study of polyethylene glycerol-utilizing bacteria: emended description of the genus Sphingomonas and new descriptions of Sphingomonas macrogoltabidus sp. nov., Sphingomonas sanguis sp. nov. and Sphingomonas terrae sp. nov.. Syst Appl Microbiol 16:227–238 [View Article]
    [Google Scholar]
  31. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417[PubMed]
    [Google Scholar]
  32. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  34. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  35. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  36. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  37. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119[PubMed] [CrossRef]
    [Google Scholar]
  38. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [View Article][PubMed]
    [Google Scholar]
  39. Zhang D.-C., Schumann P., Liu H.-C., Xin Y.-H., Zhou Y.-G., Schinner F., Margesin R. 2010; Arthrobacter alpinus sp. nov., a psychrophilic bacterium isolated from alpine soil. Int J Syst Evol Microbiol 60:2149–2153 [View Article][PubMed]
    [Google Scholar]
  40. Zhang D.-C., Busse H.-J., Liu H.-C., Zhou Y.-G., Schinner F., Margesin R. 2011; Sphingomonas glacialis sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 61:587–591 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.035964-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035964-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error