1887

Abstract

A Gram-positive, non-spore-forming actinobacterium (01-Gi-040) isolated from an indoor wall was studied to examine its taxonomic position. The isolate formed a very rudimentary substrate-mycelium that fragmented into rod-shaped to coccoid cells. On the basis of the 16S rRNA gene sequence similarity studies, strain 01-Gi-040 was shown to belong to the genus closely related to K22-20 (97.1 %), DSM 12362 (96.2 %) and LW6 (96.1 %). A close relationship was also found with CCUG 47306 (95.9 %) and Kis4-19 (95.7 %) and a moderate relationship to the type strains of the genus (94.0–94.1 %). The predominant menaquinone of strain 01-Gi-040 was MK-8(H). The peptidoglycan contained ornithine as the diagnostic diamino acid. The polar lipid profile consisted of the lipids phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, an unknown aminolipid and two unknown phosphoglycolipids. The major fatty acids iso-C, iso-C and iso-C were consistent with the fatty acid patterns reported for members of the genus . The results of DNA–DNA hybridizations, physiological and biochemical tests allowed phenotypic differentiation of strain 01-Gi-040 from the three recognized species of the genus . Strain 01-Gi-040 represents a novel species of the genus , for which we propose the name sp. nov., with the type strain 01-Gi-040 ( = DSM 22056 = CCM 7610).

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2013-01-01
2019-10-22
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