1887

Abstract

Two strains of purple non-sulfur bacteria (A-36s and A-51s) were isolated from brackish steppe soda lakes of southern Siberia. Genetically, the isolates were related most closely to the type strains of and from which they differed at the species level (98.5 % 16S rRNA gene sequence similarity, 40–53 % DNA–DNA relatedness). Cells of the two strains were ovoid to rod-shaped, 0.4–0.8 µm wide and 1.0–2.5 µm long, and motile by means of a polar flagellum. They contained internal photosynthetic membranes of vesicular type and photosynthetic pigments (bacteriochlorophyll and carotenoids of the spheroidene series). The strains were obligate haloalkaliphiles, growing over wide ranges of salinity (0.3–10.0 % NaCl) and pH (7.5–10.0), with growth optima at 1.0–3.0 % NaCl and pH 8.5–9.0. Photoheterotrophic and chemoheterotrophic growth occurred with a number of organic compounds and biotin, -aminobenzoate, thiamine and niacin as growth factors. No anaerobic respiration on nitrite, nitrate or fumarate and no fermentation were demonstrated. The strains grew photolithoautotrophically and chemolithoautotrophically with sulfide, sulfur and thiosulfate, oxidizing them to sulfate. Sulfide was oxidized via deposition of extracellular elemental sulfur. No growth with H as the electron donor was observed. The major fatty acid was C (78 %). The major quinone was ubiquinone Q-10. The DNA G+C content of strain A-36s was 65.4 mol% ( ). According to genotypic and phenotypic characteristics, the investigated strains were assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A-36s ( = VKM B-2491 = ATCC BAA-1573), which was isolated from steppe soda lake Sul’fatnoe (Zabaikal’skii Krai, southern Siberia, Russia).

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2012-12-01
2019-10-19
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References

  1. Beloborodova N. V., Osipov G. A.. ( 2000;). Small molecules originating from microbes (SMOM) and their role in microbes–host relationship. . Microb Ecol Health Dis 12:, 12–21. [CrossRef]
    [Google Scholar]
  2. Hansen T. A., Veldkamp H.. ( 1973;). Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria. . Arch Mikrobiol 92:, 45–58. [CrossRef][PubMed]
    [Google Scholar]
  3. Hiraishi A., Ueda Y.. ( 1995;). Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. . Int J Syst Bacteriol 45:, 319–326. [CrossRef][PubMed]
    [Google Scholar]
  4. Imhoff J. F.. ( 2005;). Genus Rhodovulum. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, pp. 205–209. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;. [CrossRef]
    [Google Scholar]
  5. Kompantseva E. I., Sorokin D. Iu., Gorlenko V. M., Namsaraev B. B.. ( 2005;). [The phototrophic community found in Lake Khilganta (an alkaline saline lake located in the southeastern Transbaikal region)]. . Mikrobiologiia 74:, 410–419 (in Russian).[PubMed]
    [Google Scholar]
  6. Kompantseva E. I., Briantseva I. A., Komova A. V., Namsaraev B. B.. ( 2007;). [The structure of phototrophic communities of soda lakes of the southeastern Transbaikal region]. . Mikrobiologiia 76:, 243–252 (in Russian).[PubMed]
    [Google Scholar]
  7. Kompantseva E. I., Komova A. V., Krauzova V. I., Kolganova T. V., Panteleeva A. V.. ( 2009;). [Purple nonsulfur bacteria in weakly and moderately mineralized soda lakes of the southern Transbaikal region and northeastern Mongolia]. . Mikrobiologiia 78:, 246–253 (in Russian).
    [Google Scholar]
  8. Kompantseva E. I., Komova A. V., Kostrikina N. A.. ( 2010;). Rhodovulum steppense sp. nov., an obligately haloalkaliphilic purple nonsulfur bacterium widespread in saline soda lakes of Central Asia. . Int J Syst Evol Microbiol 60:, 1210–1214. [CrossRef][PubMed]
    [Google Scholar]
  9. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  10. Owen R. J., Lapage S. P.. ( 1976;). The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. . J Appl Bacteriol 41:, 335–340. [CrossRef][PubMed]
    [Google Scholar]
  11. Pfennig N., Lippert K. D.. ( 1966;). Über das Vitamin B12-Bedürfnis phototropher Schwefelbacterien. . Arch Mikrobiol 55:, 245–256. [CrossRef]
    [Google Scholar]
  12. Ryter A., Kellenberger E., Birchandersen A., Maaloe O.. ( 1958;). [Electron microscopic study on plasmas containing desoxyribonucleic acid. I. Nucleoids of actively growing bacteria]. . Z Naturforsch B 13B:, 597–605 (in French).[PubMed]
    [Google Scholar]
  13. Sprott G. D., Larocque S., Cadotte N., Dicaire C. J., McGee M., Brisson J. R.. ( 2003;). Novel polar lipids of halophilic eubacterium Planococcus H8 and archaeon Haloferax volcanii. . Biochim Biophys Acta 1633:, 179–188. [CrossRef][PubMed]
    [Google Scholar]
  14. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  15. Vaskovsky V. E., Kostetsky E. Y.. ( 1968;). Modified spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res 9:, 396.[PubMed]
    [Google Scholar]
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