1887

Abstract

Two strains of purple non-sulfur bacteria (A-36s and A-51s) were isolated from brackish steppe soda lakes of southern Siberia. Genetically, the isolates were related most closely to the type strains of and from which they differed at the species level (98.5 % 16S rRNA gene sequence similarity, 40–53 % DNA–DNA relatedness). Cells of the two strains were ovoid to rod-shaped, 0.4–0.8 µm wide and 1.0–2.5 µm long, and motile by means of a polar flagellum. They contained internal photosynthetic membranes of vesicular type and photosynthetic pigments (bacteriochlorophyll and carotenoids of the spheroidene series). The strains were obligate haloalkaliphiles, growing over wide ranges of salinity (0.3–10.0 % NaCl) and pH (7.5–10.0), with growth optima at 1.0–3.0 % NaCl and pH 8.5–9.0. Photoheterotrophic and chemoheterotrophic growth occurred with a number of organic compounds and biotin, -aminobenzoate, thiamine and niacin as growth factors. No anaerobic respiration on nitrite, nitrate or fumarate and no fermentation were demonstrated. The strains grew photolithoautotrophically and chemolithoautotrophically with sulfide, sulfur and thiosulfate, oxidizing them to sulfate. Sulfide was oxidized via deposition of extracellular elemental sulfur. No growth with H as the electron donor was observed. The major fatty acid was C (78 %). The major quinone was ubiquinone Q-10. The DNA G+C content of strain A-36s was 65.4 mol% ( ). According to genotypic and phenotypic characteristics, the investigated strains were assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A-36s ( = VKM B-2491 = ATCC BAA-1573), which was isolated from steppe soda lake Sul’fatnoe (Zabaikal’skii Krai, southern Siberia, Russia).

Funding
This study was supported by the:
  • Russian Foundation for Basic Research (Award 07-04-00651)
  • Program of Presidium of the Russian Academy of Sciences ‘Origin and Evolution of the Biosphere’
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2012-12-01
2024-04-24
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