1887

Abstract

A strain of thermotolerant actinomycete, designated YIM 75948, was isolated from a soil sample in Yunnan province, China. The strain grew at 25–55 °C (optimum 37 °C). The substrate mycelium and aerial mycelium produced on Czapek’s agar were both pale yellow to white. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid, and the whole-cell sugars were mannose, ribose, glucose, galactose and arabinose. The major fatty acids were iso-C, iso-C and C and the predominant respiratory quinone was MK-9(H) The polar lipids consisted of phosphatidylethanolamine, phosphatidylethanolamine with hydroxy fatty acids, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. The genomic DNA G+C content was 73.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 75948 belonged in the genus and that its closest relative among recognized species was DSM 43855 (97.6 % sequence similarity). The mean level of DNA–DNA relatedness between the novel strain and DSM 43855 was, however, only 47.8 %. Based on the phenotypic, chemotaxonomic and phylogenetic data and the results of the DNA–DNA hybridizations, strain YIM 75948 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is YIM 75948 ( = JCM 17695 = DSM 45613). The description of the genus is emended to reflect the fact that the genomic DNA G+C contents of DSM 43855 and the type strain of sp. nov. recorded in the present study fell above the range given in previous descriptions of this genus.

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2012-08-01
2019-10-23
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