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Abstract

A Gram-positive, motile, short rod-shaped, orange pigmented bacterium, designated strain IMTB-3094, was isolated from a water sample collected from Tikkar Tal Lake, Haryana, and subjected to detailed polyphasic taxonomic analysis. Strain IMTB-3094 possessed most of the phenotypic and chemotaxonomic properties of the genus and, based on 16S rRNA gene sequence analysis, was assigned to this genus. Strain IMTB-3094 exhibited the highest 16S rRNA gene sequence similarity to MTCC 7759 (99.5 %) followed by MTCC 6414 (99.1 %), MTCC 7750 (98.0 %), MTCC 10851 (98.0 %) and MTCC 7751 (98.0 %). The G+C content of the genomic DNA of strain IMTB-3094 was 53.2 mol% and a DNA–DNA relatedness study confirmed that it represents a novel species. The major fatty acids of strain IMTB-3094 were iso-C (16.1 %), anteiso-C (19.0 %), iso-C (11.9 %), iso-C (9.8 %) and iso-C (12.7 %). The predominant quinones were MK-7 (55.0 %) and MK-6 (26.0 %) with minor amounts of MK-8 (12.0 %). Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain IMTB-3094 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMTB-3094 ( = MTCC 10958  = JCM 17977).

Funding
This study was supported by the:
  • , Council of Scientific and Industrial Research , (Award NWP-006)
  • , IMTECH , (Award 041/2011)
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2012-09-01
2020-12-01
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