@article{mbs:/content/journal/ijsem/10.1099/ijs.0.035782-0, author = "Mykytczuk, Nadia C. S. and Wilhelm, Roland C. and Whyte, Lyle G.", title = "Planococcus halocryophilus sp. nov., an extreme sub-zero species from high Arctic permafrost", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2012", volume = "62", number = "Pt_8", pages = "1937-1944", doi = "https://doi.org/10.1099/ijs.0.035782-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.035782-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel aerobic, Gram-positive, motile, coccoid bacterial strain, designated Or1T, was isolated from permafrost active-layer soil collected from the Canadian high Arctic. Strain Or1T was capable of growth over a broad temperature range, including sub-zero growth (below −10 to 37 °C), and at high salinity (0–19 % NaCl), growing optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % NaCl. Its taxonomic and phylogenetic position was determined by using a polyphasic approach, which indicated that strain Or1T was a member of the genus Planococcus . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Or1T belonged to the genus Planococcus , differing by 0.4–3.6 % from the type strains of all recognized Planococcus species, and was related most closely to Planococcus antarcticus CMS 26orT (98.8 % similarity) and Planococcus donghaensis JH1T (99.6 %). However, DNA–DNA hybridization experiments showed that strain Or1T had low genomic relatedness to Planococcus antarcticus CMS 26orT (18 %) and Planococcus donghaensis JH1T (46 %). The major menaquinones of strain Or1T were MK-7 (55 %), MK-8 (36 %) and MK-6 (9 %) and the major fatty acids were anteiso-C15 : 0, C16 : 1ω7c alcohol and anteiso-C17 : 0. The DNA G+C content of strain Or1T was 40.5 mol%. Differential phenotypic, phylogenetic and genomic data suggest that strain Or1T represents a novel species of the genus Planococcus , for which the name Planococcus halocryophilus sp. nov. is proposed. The type strain is Or1T ( = DSM 24743T = JCM 17719T).", }