1887

Abstract

A novel aerobic, Gram-positive, motile, coccoid bacterial strain, designated Or1, was isolated from permafrost active-layer soil collected from the Canadian high Arctic. Strain Or1 was capable of growth over a broad temperature range, including sub-zero growth (below −10 to 37 °C), and at high salinity (0–19 % NaCl), growing optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % NaCl. Its taxonomic and phylogenetic position was determined by using a polyphasic approach, which indicated that strain Or1 was a member of the genus . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Or1 belonged to the genus , differing by 0.4–3.6 % from the type strains of all recognized species, and was related most closely to CMS 26or (98.8 % similarity) and JH1 (99.6 %). However, DNA–DNA hybridization experiments showed that strain Or1 had low genomic relatedness to CMS 26or (18 %) and JH1 (46 %). The major menaquinones of strain Or1 were MK-7 (55 %), MK-8 (36 %) and MK-6 (9 %) and the major fatty acids were anteiso-C, Cω7 alcohol and anteiso-C. The DNA G+C content of strain Or1 was 40.5 mol%. Differential phenotypic, phylogenetic and genomic data suggest that strain Or1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Or1 ( = DSM 24743 = JCM 17719).

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2012-08-01
2019-09-22
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References

  1. Alam S. I., Singh L., Dube S., Reddy G. S., Shivaji S.. ( 2003;). Psychrophilic Planococcus maitriensis sp. nov. from Antarctica. . Syst Appl Microbiol 26:, 505–510. [CrossRef][PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  3. Choi J.-H., Im W.-T., Liu Q.-M., Yoo J.-S., Shin J.-H., Rhee S.-K., Roh D.-H.. ( 2007;). Planococcus donghaensis sp. nov., a starch-degrading bacterium isolated from the East Sea, South Korea. . Int J Syst Evol Microbiol 57:, 2645–2650. [CrossRef][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  5. Dai X., Wang Y.-N., Wang B.-J., Liu S.-J., Zhou Y.-G.. ( 2005;). Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov.. Int J Syst Evol Microbiol 55:, 699–702. [CrossRef][PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  7. Deming J. W.. ( 2002;). Psychrophiles and polar regions. . Curr Opin Microbiol 5:, 301–309. [CrossRef][PubMed]
    [Google Scholar]
  8. Engelhardt M. A., Daly K., Swannell R. P. J., Head I. M.. ( 2001;). Isolation and characterization of a novel hydrocarbon-degrading, gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. nov.. J Appl Microbiol 90:, 237–247. [CrossRef][PubMed]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef][PubMed]
    [Google Scholar]
  10. Hao M. V., Komagata K.. ( 1985;). A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. . J Gen Appl Microbiol 31:, 441–455. [CrossRef]
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  12. Juck D. F., Whissell G., Steven B., Pollard W., McKay C. P., Greer C. W., Whyte L. G.. ( 2005;). Utilization of fluorescent microspheres and a green fluorescent protein-marked strain for assessment of microbiological contamination of permafrost and ground ice core samples from the Canadian High Arctic. . Appl Environ Microbiol 71:, 1035–1041. [CrossRef][PubMed]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  14. Jung Y.-T., Kang S.-J., Oh T.-K., Yoon J.-H., Kim B.-H.. ( 2009;). Planomicrobium flavidum sp. nov., isolated from a marine solar saltern, and transfer of Planococcus stackebrandtii Mayilraj et al. 2005 to the genus Planomicrobium as Planomicrobium stackebrandtii comb. nov.. Int J Syst Evol Microbiol 59:, 2929–2933. [CrossRef][PubMed]
    [Google Scholar]
  15. Junge K., Gosink J. J., Hoppe H. G., Staley J. T.. ( 1998;). Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic sea ice brine. Description of Planococcus mcmeekinii, sp. nov.. Syst Appl Microbiol 21:, 306–314. [CrossRef][PubMed]
    [Google Scholar]
  16. Kocur M., Zdena P., Hodgkiss W., Martinec T.. ( 1970;). The taxonomic status of the genus Planococcus Migula 1894. . Int J Syst Bacteriol 20:, 241–248. [CrossRef]
    [Google Scholar]
  17. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  18. MacKenzie S. L.. ( 1987;). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. . J Assoc Off Anal Chem 70:, 151–160.[PubMed]
    [Google Scholar]
  19. Mayilraj S., Prasad G. S., Suresh K., Saini H. S., Shivaji S., Chakrabarti T.. ( 2005;). Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. . Int J Syst Evol Microbiol 55:, 91–94. [CrossRef][PubMed]
    [Google Scholar]
  20. Migula W.. ( 1894;). Über ein neues System der Bakterien. . Arb Bakteriol Inst Karlsruhe 1:, 235–238 (in German).
    [Google Scholar]
  21. Nakagawa Y., Sakane T., Yokota A.. ( 1996;). Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov.. Int J Syst Bacteriol 46:, 866–870. [CrossRef][PubMed]
    [Google Scholar]
  22. Reddy G. S. N., Prakash J. S. S., Vairamani M., Prabhakar S., Matsumoto G. I., Shivaji S.. ( 2002;). Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. . Extremophiles 6:, 253–261. [CrossRef][PubMed]
    [Google Scholar]
  23. Romano I., Giordano A., Lama L., Nicolaus B., Gambacorta A.. ( 2003;). Planococcus rifietensis sp. nov., isolated from algal mat collected from a sulfurous spring in Campania (Italy). . Syst Appl Microbiol 26:, 357–366. [CrossRef][PubMed]
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  26. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  28. Steven B., Briggs G., McKay C. P., Pollard W. H., Greer C. W., Whyte L. G.. ( 2007;). Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods. . FEMS Microbiol Ecol 59:, 513–523. [CrossRef][PubMed]
    [Google Scholar]
  29. Steven B., Chen M. Q., Greer C. W., Whyte L. G., Niederberger T. D.. ( 2008;). Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost. . Int J Syst Evol Microbiol 58:, 1497–1501. [CrossRef][PubMed]
    [Google Scholar]
  30. Suresh K., Mayilraj S., Bhattacharya A., Chakrabarti T.. ( 2007;). Planococcus columbae sp. nov., isolated from pigeon faeces. . Int J Syst Evol Microbiol 57:, 1266–1271. [CrossRef][PubMed]
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  32. Vali H., Weiss B., Li Y.-L., Sears S. K., Kim S. S., Kirschvink J. L., Zhang C. L.. ( 2004;). Formation of tabular single-domain magnetite induced by Geobacter metallireducens GS-15. . Proc Natl Acad Sci U S A 101:, 16121–16126. [CrossRef][PubMed]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  34. Yoon J.-H., Kang S.-S., Lee K.-C., Lee E. S., Kho Y. H., Kang K. H., Park Y. H.. ( 2001;). Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al. 1996) and Planococcus mcmeekinii (Junge et al., 1998) to the genus Planomicrobium. . Int J Syst Evol Microbiol 51:, 1511–1520.[PubMed]
    [Google Scholar]
  35. Yoon J.-H., Weiss N., Kang K. H., Oh T.-K., Park Y.-H.. ( 2003;). Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. . Int J Syst Evol Microbiol 53:, 2013–2017. [CrossRef][PubMed]
    [Google Scholar]
  36. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh K.-H., Oh T.-K.. ( 2010;). Planococcus salinarum sp. nov., isolated from a marine solar saltern, and emended description of the genus Planococcus. . Int J Syst Evol Microbiol 60:, 754–758. [CrossRef][PubMed]
    [Google Scholar]
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