1887

Abstract

A novel aerobic, Gram-positive, motile, coccoid bacterial strain, designated Or1, was isolated from permafrost active-layer soil collected from the Canadian high Arctic. Strain Or1 was capable of growth over a broad temperature range, including sub-zero growth (below −10 to 37 °C), and at high salinity (0–19 % NaCl), growing optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % NaCl. Its taxonomic and phylogenetic position was determined by using a polyphasic approach, which indicated that strain Or1 was a member of the genus . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Or1 belonged to the genus , differing by 0.4–3.6 % from the type strains of all recognized species, and was related most closely to CMS 26or (98.8 % similarity) and JH1 (99.6 %). However, DNA–DNA hybridization experiments showed that strain Or1 had low genomic relatedness to CMS 26or (18 %) and JH1 (46 %). The major menaquinones of strain Or1 were MK-7 (55 %), MK-8 (36 %) and MK-6 (9 %) and the major fatty acids were anteiso-C, Cω7 alcohol and anteiso-C. The DNA G+C content of strain Or1 was 40.5 mol%. Differential phenotypic, phylogenetic and genomic data suggest that strain Or1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Or1 ( = DSM 24743 = JCM 17719).

Funding
This study was supported by the:
  • , Natural Sciences and Engineering Research Council (NSERC)
  • , Canada Research Chair program (CRC)
  • , Canadian Foundation for Innovation (CFI)
  • , Polar Continental Shelf Program (PCSP)
  • , Canadian Space Agency (CSA) Canadian Analogue Research Network (CARN)
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2012-08-01
2021-03-02
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