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Volume 62, Issue Pt_9

gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake Free

Abstract

Two pink, non-motile, aerobic, alkaliphilic, halotolerant, Gram-negative cocci, designated MIM28 and MIM29, were isolated from the surface water of a haloalkaline lake on the Mongolia Plateau. The isolates grew optimally at 30–33 °C, at pH 8–9 and with 3–4 % (w/v) NaCl. The isolates were chemoheterotrophic and could assimilate carbohydrates, organic acids and amino acids. The major respiratory quinone was menaquinone MK-7. The major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The predominant cellular fatty acids were iso-C (13.8–17.5 %), anteiso-C (10.5–11.2 %), iso-C (9.9–13.0 %), C (4.3–4.6 %), iso-C (3.8–5.3 %), anteiso-C (3.7–7.1 %), Cω6 (4.6–6.4 %), iso-C 3-OH (4.6–5.8 %), summed feature 3 (Cω7 and/or Cω6; 4.0–6.4 %) and summed feature 9 (iso-Cω9 and/or C 10-methyl; 10.4–12.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were most closely related to YIM CH208 (93.6 % 16S rRNA gene sequence similarity), the genus (90.4–92 %) and other members of the family (87.8–90 %). The DNA G+C contents of strains MIM28 and MIM29 were 62.8 and 62.2 mol%. On the basis of morphology, physiology, fatty acid composition, phylogeny and 16S rRNA gene sequence analysis, the isolates are assigned to a novel species of a new genus, for which we propose the name gen. nov., sp. nov.; the type strain of the type species is MIM28 ( = ACCC 05511  = KCTC 19808).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (NSFC) (Award 30860015 and 30760004)
  • Major Program of Natural Science Foundation of Inner Mongolia (Award 2009ZD03)
  • Innovative Research Group Fund of Inner Mongolia Agriculture University (Award NDPYTD2010-3)
© 2012 IUMS
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Mongoliicoccus roseus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake
Int J Syst Evol Microbiol 62, 2206 (2012); https://doi.org/10.1099/ijs.0.035766-0
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