1887

Abstract

Two pink, non-motile, aerobic, alkaliphilic, halotolerant, Gram-negative cocci, designated MIM28 and MIM29, were isolated from the surface water of a haloalkaline lake on the Mongolia Plateau. The isolates grew optimally at 30–33 °C, at pH 8–9 and with 3–4 % (w/v) NaCl. The isolates were chemoheterotrophic and could assimilate carbohydrates, organic acids and amino acids. The major respiratory quinone was menaquinone MK-7. The major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The predominant cellular fatty acids were iso-C (13.8–17.5 %), anteiso-C (10.5–11.2 %), iso-C (9.9–13.0 %), C (4.3–4.6 %), iso-C (3.8–5.3 %), anteiso-C (3.7–7.1 %), Cω6 (4.6–6.4 %), iso-C 3-OH (4.6–5.8 %), summed feature 3 (Cω7 and/or Cω6; 4.0–6.4 %) and summed feature 9 (iso-Cω9 and/or C 10-methyl; 10.4–12.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were most closely related to YIM CH208 (93.6 % 16S rRNA gene sequence similarity), the genus (90.4–92 %) and other members of the family (87.8–90 %). The DNA G+C contents of strains MIM28 and MIM29 were 62.8 and 62.2 mol%. On the basis of morphology, physiology, fatty acid composition, phylogeny and 16S rRNA gene sequence analysis, the isolates are assigned to a novel species of a new genus, for which we propose the name gen. nov., sp. nov.; the type strain of the type species is MIM28 ( = ACCC 05511  = KCTC 19808).

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2012-09-01
2019-10-22
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Anil Kumar P. , Srinivas T. N. R. , Madhu S. , Manorama R. , Shivaji S. . ( 2010a; ). Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. . Int J Syst Evol Microbiol 60:, 721–726. [CrossRef] [PubMed]
    [Google Scholar]
  3. Anil Kumar P. , Srinivas T. N. R. , Pavan Kumar P. , Madhu S. , Shivaji S. . ( 2010b; ). Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes . . Int J Syst Evol Microbiol 60:, 2320–2325. [CrossRef] [PubMed]
    [Google Scholar]
  4. Antón J. , Oren A. , Benlloch S. , Rodríguez-Valera F. , Amann R. , Rosselló-Mora R. . ( 2002; ). Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. . Int J Syst Evol Microbiol 52:, 485–491.[PubMed]
    [Google Scholar]
  5. Arun A. B. , Young C.-C. , Chen W.-M. , Hung M.-H. , Lai W.-A. , Chou J.-H. , Rekha P. D. , Shen F.-T. , Su S. P. . ( 2009; ). Belliella pelovolcani sp. nov., isolated from a mud-volcano in Taiwan. . Int J Syst Evol Microbiol 59:, 2534–2537. [CrossRef] [PubMed]
    [Google Scholar]
  6. Bauer A. W. , Kirby W. M. M. , Sherris J. C. , Turck M. . ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496.[PubMed]
    [Google Scholar]
  7. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  8. Bowman J. P. , Nichols C. M. , Gibson J. A. E. . ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. . Int J Syst Evol Microbiol 53:, 1343–1355. [CrossRef] [PubMed]
    [Google Scholar]
  9. Brettar I. , Christen R. , Höfle M. G. . ( 2004a; ). Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. . Int J Syst Evol Microbiol 54:, 2335–2341. [CrossRef] [PubMed]
    [Google Scholar]
  10. Brettar I. , Christen R. , Höfle M. G. . ( 2004b; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. . Int J Syst Evol Microbiol 54:, 65–70. [CrossRef] [PubMed]
    [Google Scholar]
  11. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform 252 bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  12. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  13. Cottrell M. T. , Kirchman D. L. . ( 2000; ). Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. . Appl Environ Microbiol 66:, 5116–5122. [CrossRef] [PubMed]
    [Google Scholar]
  14. Du H. L. , Jiao N. Z. , Hu Y. H. , Zeng Y. H. . ( 2006; ). Diversity and distribution of pigmented heterotrophic bacteria in marine environments. . FEMS Microbiol Ecol 57:, 92–105. [CrossRef] [PubMed]
    [Google Scholar]
  15. Felsenstein J. . ( 1989; ). phylip – phylogeny inference package (version 3.2). . Cladistics 5:, 164–166.
    [Google Scholar]
  16. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Grant W. D. , Mwatha W. E. , Jones B. E. . ( 1990; ). Alkaliphiles: ecology, diversity and application. . FEMS Microbiol Rev 75:, 255–269. [CrossRef]
    [Google Scholar]
  18. Groth I. , Schumann P. , Rainey F. A. , Martin K. , Schuetze B. , Augsten K. . ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  19. Höfle M. G. . ( 1992; ). Bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-RNA analysis. . Appl Environ Microbiol 58:, 3387–3394.[PubMed]
    [Google Scholar]
  20. Huß V. A. R. , Festl H. , Schleifer K.-H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef]
    [Google Scholar]
  21. Jones B. E. , Grant W. D. , Duckworth A. W. , Owenson G. G. . ( 1998; ). Microbial diversity of soda lakes. . Extremophiles 2:, 191–200. [CrossRef] [PubMed]
    [Google Scholar]
  22. Joshi A. A. , Kanekar P. P. , Kelkar A. S. , Shouche Y. S. , Vani A. A. , Borgave S. B. , Sarnaik S. S. . ( 2008; ). Cultivable bacterial diversity of alkaline Lonar lake, India. . Microb Ecol 55:, 163–172. [CrossRef] [PubMed]
    [Google Scholar]
  23. Kämpfer P. , Young C. C. , Chen W. M. , Rekha P. D. , Fallschissel K. , Lodders N. , Chou J.-H. , Shen F.-T. , Frischmann A. . & other authors ( 2010; ). Fontibacter flavus gen. nov., sp. nov., a member of the family ‘Cyclobacteriaceae’, isolated from a hot spring. . Int J Syst Evol Microbiol 60:, 2066–2070. [CrossRef]
    [Google Scholar]
  24. Kim H. , Joung Y. , Ahn T.-S. , Joh K. . ( 2011; ). Echinicola jeungdonensis sp. nov., isolated from a solar saltern in Korea. . Int J Syst Evol Microbiol 61:, 2065–2068. [CrossRef]
    [Google Scholar]
  25. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  26. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  27. Kumar S. , Nei M. , Dudley J. , Tamura K. . ( 2008; ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9:, 299–306. [CrossRef] [PubMed]
    [Google Scholar]
  28. Liu Y. , Li H. , Jiang J.-T. , Liu Y.-H. , Song X.-F. , Xu C.-J. , Liu Z.-P. . ( 2009; ). Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. . Int J Syst Evol Microbiol 59:, 1759–1763. [CrossRef] [PubMed]
    [Google Scholar]
  29. Ludwig W. , Euzéby J. , Whitman W. B. . ( 2010; ). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes . . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4. Edited by Krieg N. R. , Staley J. T. , Brown D. R. , Hedlund B. P. , Paster B. J. , Ward N. L. , Ludwig W. , Whitman W. B. . . New York:: Springer;.
    [Google Scholar]
  30. Ma Y. H. , Zhang W. Z. , Xue Y. F. , Zhou P. J. , Ventosa A. , Grant W. D. . ( 2004; ). Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses. . Extremophiles 8:, 45–51. [CrossRef] [PubMed]
    [Google Scholar]
  31. Nedashkovskaya O. I. , Suzuki M. , Vysotskii M. V. , Mikhailov V. V. . ( 2003; ). Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . . Int J Syst Evol Microbiol 53:, 81–85. [CrossRef] [PubMed]
    [Google Scholar]
  32. Nedashkovskaya O. I. , Kim S. B. , Lee M. S. , Park M. S. , Lee K. H. , Lysenko A. M. , Oh H. W. , Mikhailov V. V. , Bae K. S. . ( 2005; ). Cyclobacterium amurskyense sp. nov., a novel marine bacterium isolated from sea water. . Int J Syst Evol Microbiol 55:, 2391–2394. [CrossRef] [PubMed]
    [Google Scholar]
  33. Nedashkovskaya O. I. , Kim S. B. , Vancanneyt M. , Lysenko A. M. , Shin D. S. , Park M. S. , Lee K. H. , Jung W. J. , Kalinovskaya N. I. . & other authors ( 2006; ). Echinicola pacifica gen. nov., sp. nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius . . Int J Syst Evol Microbiol 56:, 953–958. [CrossRef] [PubMed]
    [Google Scholar]
  34. Nedashkovskaya O. I. , Kim S. B. , Hoste B. , Shin D. S. , Beleneva I. A. , Vancanneyt M. , Mikhailov V. V. . ( 2007; ). Echinicola vietnamensis sp. nov., a member of the phylum Bacteroidetes isolated from seawater. . Int J Syst Evol Microbiol 57:, 761–763. [CrossRef] [PubMed]
    [Google Scholar]
  35. Raj H. D. , Maloy S. R. . ( 1990; ). Proposal of Cyclobacterium marinum gen. nov., comb. nov. for a marine bacterium previously assigned to the genus Flectobacillus . . Int J Syst Bacteriol 40:, 337–347. [CrossRef]
    [Google Scholar]
  36. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI.
  37. Sly L. I. , Blackall L. L. , Kraat P. C. , Tian-Shen T. , Sangkhobol V. . ( 1986; ). The use of second derivate plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. . J Microbiol Methods 5:, 139–156. [CrossRef]
    [Google Scholar]
  38. Tian S.-P. , Wang Y.-X. , Hu B. , Zhang X.-X. , Xiao W. , Chen Y. , Lai Y.-H. , Wen M.-L. , Cui X.-L. . ( 2010; ). Litoribacter ruber gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment. . Int J Syst Evol Microbiol 60:, 2996–3001. [CrossRef] [PubMed]
    [Google Scholar]
  39. Tindall B. J. , Rosselló-Móra R. , Busse H. J. , Ludwig W. , Kämpfer P. . ( 2010; ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef] [PubMed]
    [Google Scholar]
  40. Wang Y.-X. , Liu J.-H. , Xiao W. , Zhang X.-X. , Li Y.-Q. , Lai Y.-H. . & other authors ( 2012; ). Fodinibius salinus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine. . Int J Syst Bacteriol 62:, 390–396. [CrossRef]
    [Google Scholar]
  41. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  42. Wisotzkey J. D. , Jurtshuk P. Jr , Fox G. E. . ( 1990; ). PCR amplification of 16S rDNA from lyophilized cell cultures facilitates studies in molecular systematics. . Curr Microbiol 21:, 325–327. [CrossRef] [PubMed]
    [Google Scholar]
  43. Yang C.-X. , Liu Y.-P. , Bao Q.-H. , Feng F.-Y. , Liu H.-R. , Zhang X.-J. , Zhao Y.-L. . ( 2012; ). Mongoliitalea lutea gen. nov., sp. nov., a novel alkaliphilic and halotolerant bacterium isolated from a haloalkaline lake on the Mongolia Plateau. . Int J Syst Evol Microbiol 62:, 647–653. [CrossRef]
    [Google Scholar]
  44. Ying J.-Y. , Wang B.-J. , Yang S.-S. , Liu S.-J. . ( 2006; ). Cyclobacterium lianum sp. nov., a marine bacterium isolated from sediment of an oilfield in the South China Sea, and emended description of the genus Cyclobacterium . . Int J Syst Evol Microbiol 56:, 2927–2930. [CrossRef] [PubMed]
    [Google Scholar]
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