Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_9

gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake Free

Abstract

Two pink, non-motile, aerobic, alkaliphilic, halotolerant, Gram-negative cocci, designated MIM28 and MIM29, were isolated from the surface water of a haloalkaline lake on the Mongolia Plateau. The isolates grew optimally at 30–33 °C, at pH 8–9 and with 3–4 % (w/v) NaCl. The isolates were chemoheterotrophic and could assimilate carbohydrates, organic acids and amino acids. The major respiratory quinone was menaquinone MK-7. The major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The predominant cellular fatty acids were iso-C (13.8–17.5 %), anteiso-C (10.5–11.2 %), iso-C (9.9–13.0 %), C (4.3–4.6 %), iso-C (3.8–5.3 %), anteiso-C (3.7–7.1 %), Cω6 (4.6–6.4 %), iso-C 3-OH (4.6–5.8 %), summed feature 3 (Cω7 and/or Cω6; 4.0–6.4 %) and summed feature 9 (iso-Cω9 and/or C 10-methyl; 10.4–12.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were most closely related to YIM CH208 (93.6 % 16S rRNA gene sequence similarity), the genus (90.4–92 %) and other members of the family (87.8–90 %). The DNA G+C contents of strains MIM28 and MIM29 were 62.8 and 62.2 mol%. On the basis of morphology, physiology, fatty acid composition, phylogeny and 16S rRNA gene sequence analysis, the isolates are assigned to a novel species of a new genus, for which we propose the name gen. nov., sp. nov.; the type strain of the type species is MIM28 ( = ACCC 05511  = KCTC 19808).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (NSFC) (Award 30860015 and 30760004)
  • Major Program of Natural Science Foundation of Inner Mongolia (Award 2009ZD03)
  • Innovative Research Group Fund of Inner Mongolia Agriculture University (Award NDPYTD2010-3)
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035766-0
2012-09-01
2025-03-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2206.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035766-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
     [Google Scholar]
  2. Anil Kumar P., Srinivas T. N. R., Madhu S., Manorama R., Shivaji S. 2010a; Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol 60:721–726 [View Article][PubMed]
     [Google Scholar]
  3. Anil Kumar P., Srinivas T. N. R., Pavan Kumar P., Madhu S., Shivaji S. 2010b; Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes . Int J Syst Evol Microbiol 60:2320–2325 [View Article][PubMed]
     [Google Scholar]
  4. Antón J., Oren A., Benlloch S., Rodríguez-Valera F., Amann R., Rosselló-Mora R. 2002; Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52:485–491[PubMed]
     [Google Scholar]
  5. Arun A. B., Young C.-C., Chen W.-M., Hung M.-H., Lai W.-A., Chou J.-H., Rekha P. D., Shen F.-T., Su S. P. 2009; Belliella pelovolcani sp. nov., isolated from a mud-volcano in Taiwan. Int J Syst Evol Microbiol 59:2534–2537 [View Article][PubMed]
     [Google Scholar]
  6. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
     [Google Scholar]
  7. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
     [Google Scholar]
  8. Bowman J. P., Nichols C. M., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53:1343–1355 [View Article][PubMed]
     [Google Scholar]
  9. Brettar I., Christen R., Höfle M. G. 2004a; Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54:2335–2341 [View Article][PubMed]
     [Google Scholar]
  10. Brettar I., Christen R., Höfle M. G. 2004b; Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54:65–70 [View Article][PubMed]
     [Google Scholar]
  11. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform 252 bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [View Article]
     [Google Scholar]
  12. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
     [Google Scholar]
  13. Cottrell M. T., Kirchman D. L. 2000; Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 66:5116–5122 [View Article][PubMed]
     [Google Scholar]
  14. Du H. L., Jiao N. Z., Hu Y. H., Zeng Y. H. 2006; Diversity and distribution of pigmented heterotrophic bacteria in marine environments. FEMS Microbiol Ecol 57:92–105 [View Article][PubMed]
     [Google Scholar]
  15. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2). Cladistics 5:164–166
     [Google Scholar]
  16. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
     [Google Scholar]
  17. Grant W. D., Mwatha W. E., Jones B. E. 1990; Alkaliphiles: ecology, diversity and application. FEMS Microbiol Rev 75:255–269 [View Article]
     [Google Scholar]
  18. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
     [Google Scholar]
  19. Höfle M. G. 1992; Bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-RNA analysis. Appl Environ Microbiol 58:3387–3394[PubMed]
     [Google Scholar]
  20. Huß V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article]
     [Google Scholar]
  21. Jones B. E., Grant W. D., Duckworth A. W., Owenson G. G. 1998; Microbial diversity of soda lakes. Extremophiles 2:191–200 [View Article][PubMed]
     [Google Scholar]
  22. Joshi A. A., Kanekar P. P., Kelkar A. S., Shouche Y. S., Vani A. A., Borgave S. B., Sarnaik S. S. 2008; Cultivable bacterial diversity of alkaline Lonar lake, India. Microb Ecol 55:163–172 [View Article][PubMed]
     [Google Scholar]
  23. Kämpfer P., Young C. C., Chen W. M., Rekha P. D., Fallschissel K., Lodders N., Chou J.-H., Shen F.-T., Frischmann A. other authors 2010; Fontibacter flavus gen. nov., sp. nov., a member of the family ‘Cyclobacteriaceae’, isolated from a hot spring. Int J Syst Evol Microbiol 60:2066–2070 [View Article]
     [Google Scholar]
  24. Kim H., Joung Y., Ahn T.-S., Joh K. 2011; Echinicola jeungdonensis sp. nov., isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 61:2065–2068 [View Article]
     [Google Scholar]
  25. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  26. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  27. Kumar S., Nei M., Dudley J., Tamura K. 2008; mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306 [View Article][PubMed]
     [Google Scholar]
  28. Liu Y., Li H., Jiang J.-T., Liu Y.-H., Song X.-F., Xu C.-J., Liu Z.-P. 2009; Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 59:1759–1763 [View Article][PubMed]
     [Google Scholar]
  29. Ludwig W., Euzéby J., Whitman W. B. 2010; Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes . In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 4 Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. New York: Springer;
     [Google Scholar]
  30. Ma Y. H., Zhang W. Z., Xue Y. F., Zhou P. J., Ventosa A., Grant W. D. 2004; Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses. Extremophiles 8:45–51 [View Article][PubMed]
     [Google Scholar]
  31. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:81–85 [View Article][PubMed]
     [Google Scholar]
  32. Nedashkovskaya O. I., Kim S. B., Lee M. S., Park M. S., Lee K. H., Lysenko A. M., Oh H. W., Mikhailov V. V., Bae K. S. 2005; Cyclobacterium amurskyense sp. nov., a novel marine bacterium isolated from sea water. Int J Syst Evol Microbiol 55:2391–2394 [View Article][PubMed]
     [Google Scholar]
  33. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Lysenko A. M., Shin D. S., Park M. S., Lee K. H., Jung W. J., Kalinovskaya N. I. other authors 2006; Echinicola pacifica gen. nov., sp. nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 56:953–958 [View Article][PubMed]
     [Google Scholar]
  34. Nedashkovskaya O. I., Kim S. B., Hoste B., Shin D. S., Beleneva I. A., Vancanneyt M., Mikhailov V. V. 2007; Echinicola vietnamensis sp. nov., a member of the phylum Bacteroidetes isolated from seawater. Int J Syst Evol Microbiol 57:761–763 [View Article][PubMed]
     [Google Scholar]
  35. Raj H. D., Maloy S. R. 1990; Proposal of Cyclobacterium marinum gen. nov., comb. nov. for a marine bacterium previously assigned to the genus Flectobacillus . Int J Syst Bacteriol 40:337–347 [View Article]
     [Google Scholar]
  36. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI.
  37. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. 1986; The use of second derivate plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156 [View Article]
     [Google Scholar]
  38. Tian S.-P., Wang Y.-X., Hu B., Zhang X.-X., Xiao W., Chen Y., Lai Y.-H., Wen M.-L., Cui X.-L. 2010; Litoribacter ruber gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment. Int J Syst Evol Microbiol 60:2996–3001 [View Article][PubMed]
     [Google Scholar]
  39. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
     [Google Scholar]
  40. Wang Y.-X., Liu J.-H., Xiao W., Zhang X.-X., Li Y.-Q., Lai Y.-H. other authors 2012; Fodinibius salinus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine. Int J Syst Bacteriol 62:390–396 [View Article]
     [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  42. Wisotzkey J. D., Jurtshuk P. Jr, Fox G. E. 1990; PCR amplification of 16S rDNA from lyophilized cell cultures facilitates studies in molecular systematics. Curr Microbiol 21:325–327 [View Article][PubMed]
     [Google Scholar]
  43. Yang C.-X., Liu Y.-P., Bao Q.-H., Feng F.-Y., Liu H.-R., Zhang X.-J., Zhao Y.-L. 2012; Mongoliitalea lutea gen. nov., sp. nov., a novel alkaliphilic and halotolerant bacterium isolated from a haloalkaline lake on the Mongolia Plateau. Int J Syst Evol Microbiol 62:647–653 [View Article]
     [Google Scholar]
  44. Ying J.-Y., Wang B.-J., Yang S.-S., Liu S.-J. 2006; Cyclobacterium lianum sp. nov., a marine bacterium isolated from sediment of an oilfield in the South China Sea, and emended description of the genus Cyclobacterium . Int J Syst Evol Microbiol 56:2927–2930 [View Article][PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.035766-0
Loading
Mongoliicoccus roseus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake
Int J Syst Evol Microbiol 62, 2206 (2012); https://doi.org/10.1099/ijs.0.035766-0
/content/journal/ijsem/10.1099/ijs.0.035766-0
/content/journal/ijsem/10.1099/ijs.0.035766-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error