1887

Abstract

-like bacteria were isolated from the respiratory tract of two patients in California, USA, and South Australia, but were not thought to cause disease. These bacteria, strains F2632 and IMVS-3376, were found to have identical macrophage infectivity potentiator gene sequences and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative-staining bacterial strains grew on buffered charcoal yeast extract medium, were cysteine auxotrophs and made a characteristic diffusible bright yellow fluorescent pigment, with one strain making a late appearing colony-bound blue–white fluorescent pigment. The optimal growth temperature was 35 °C, with very poor growth at 37 °C in broth or on solid media. There was no growth in human A549 cells at either 35 or 37 °C, but excellent growth in at 30 °C and poorer growth at 35 °C. Phylogenetic analysis of these bacteria was performed by sequence analysis of 16S rRNA, , ribonuclease P, ribosomal polymerase B and zinc metalloprotease genes. These studies confirmed that the new strains represented a single novel species of the genus for which the name sp. nov. is proposed. The type strain is IMVS-3376 ( = IMVS 3113 = ATCC BAA-2169).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035709-0
2012-08-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1766.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035709-0&mimeType=html&fmt=ahah

References

  1. Boulanger C. A. , Edelstein P. H. . ( 1995; ). Precision and accuracy of recovery of Legionella pneumophila from seeded tap water by filtration and centrifugation. . Appl Environ Microbiol 61:, 1805–1809.[PubMed]
    [Google Scholar]
  2. Brenner D. J. . ( 1987; ). Classification of the legionellae. . Semin Respir Infect 2:, 190–205.[PubMed]
    [Google Scholar]
  3. Cianciotto N. P. , Abu Kwaik Y. , Edelstein P. H. , Fields B. S. , Geary D. F. , Harrison T. G. , Joseph C. A. , Ratcliff R. M. , Stout J. E. . & other authors ( 2006; ). Legionella: state of the art 30 years after its recognition. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Edelstein P. H. . ( 2004;). Detection of Legionella antigen by direct immunofluorescence. . In Clinical Microbiology Procedures Handbook, pp. 11.3.1–11.3.7. Edited by Isenberg H. D. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  5. Edelstein P. H. , Cianciotto N. P. . ( 2010;). Legionella . . In Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases, , 7th edn., pp. 2969–2984. Edited by Mandell G. L. . , J. E. Bennett & R. Dolln. Philadelphia:: Elsevier;.
    [Google Scholar]
  6. Edelstein P. H. , Edelstein M. A. C. . ( 1991; ). Comparison of different agars used in the formulation of buffered charcoal yeast extract medium. . J Clin Microbiol 29:, 190–191.[PubMed]
    [Google Scholar]
  7. Edelstein P. H. , Edelstein M. A. C. . ( 1993; ). Comparison of three buffers used in the formulation of buffered charcoal yeast extract medium. . J Clin Microbiol 31:, 3329–3330.[PubMed]
    [Google Scholar]
  8. Edelstein P. H. , Brenner D. J. , Moss C. W. , Steigerwalt A. G. , Francis E. M. , George W. L. . ( 1982; ). Legionella wadsworthii species nova: a cause of human pneumonia. . Ann Intern Med 97:, 809–813.[PubMed] [CrossRef]
    [Google Scholar]
  9. Edelstein P. H. , Beer K. B. , DeBoynton E. D. . ( 1987; ). Influence of growth temperature on virulence of Legionella pneumophila . . Infect Immun 55:, 2701–2705.[PubMed]
    [Google Scholar]
  10. Higa F. , Edelstein P. H. . ( 2001; ). Potential virulence role of the Legionella pneumophila ptsP ortholog. . Infect Immun 69:, 4782–4789. [CrossRef] [PubMed]
    [Google Scholar]
  11. Huson D. H. , Bryant D. . ( 2006; ). Application of phylogenetic networks in evolutionary studies. . Mol Biol Evol 23:, 254–267. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ko K. S. , Lee H. K. , Park M. Y. , Lee K. H. , Yun Y. J. , Woo S. Y. , Miyamoto H. , Kook Y. H. . ( 2002; ). Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species. . J Clin Microbiol 40:, 2653–2658. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kodaka H. , Armfield A. Y. , Lombard G. L. , Dowell V. R. Jr . ( 1982; ). Practical procedure for demonstrating bacterial flagella. . J Clin Microbiol 16:, 948–952.[PubMed]
    [Google Scholar]
  14. Lück P. C. , Jacobs E. , Röske I. , Schröter-Bobsin U. , Dumke R. , Gronow S. . ( 2010; ). Legionella dresdenensis sp. nov. isolated from river water. . Int J Syst Evol Microbiol 60:, 2557–2562. [CrossRef] [PubMed]
    [Google Scholar]
  15. Miller M. A. , Pfeiffer W. , Schwartz T. . ( 2010; ). Creating the CIPRES science gateway for inference of large phylogenetic trees. . In Proceedings of the Gateway Computing Environments Workshop, pp. 1–8. New Orleans, LA:: Gateway Computing Environments Workshop;.
    [Google Scholar]
  16. Posada D. , Crandall K. A. . ( 1998; ). modeltest: testing the model of DNA substitution. . Bioinformatics 14:, 817–818. [CrossRef] [PubMed]
    [Google Scholar]
  17. Ratcliff R. M. , Lanser J. A. , Manning P. A. , Heuzenroeder M. W. . ( 1998; ). Sequence-based classification scheme for the genus Legionella targeting the mip gene. . J Clin Microbiol 36:, 1560–1567.[PubMed]
    [Google Scholar]
  18. Rubin C. J. , Thollesson M. , Kirsebom L. A. , Herrmann B. . ( 2005; ). Phylogenetic relationships and species differentiation of 39 Legionella species by sequence determination of the RNase P RNA gene rnpB. . Int J Syst Evol Microbiol 55:, 2039–2049. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035709-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035709-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error