1887

Abstract

Strains of the recently proposed species share more than 99.4 % 16S rRNA gene sequence similarity with the type strain of although these two species do not appear to be similar from their published descriptions. The aim of this study was to perform phenotypic and genetic analyses of both species to clarify their taxonomic position. JCM 16497 exhibited high gene sequence similarity with JCM 17136 (100 %) as well as JCM 16498 (100 %). The gene sequence analysis and levels of DNA–DNA relatedness observed demonstrated JCM 17136, JCM 16497, and JCM 16498 are members of a single species. Based on these data, we propose as a later heterotypic synonym of . An emended description of is provided.

Funding
This study was supported by the:
  • IFO (Institute for Fermentation, Osaka, Japan) (Award 2009-2011)
  • Scientific Research from the Japan Society for the Promotion of Science (Award 23580126)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035659-0
2012-06-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/6/1241.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035659-0&mimeType=html&fmt=ahah

References

  1. Clavel T., Saalfrank A., Charrier C., Haller D. 2010; Isolation of bacteria from mouse caecal samples and description of Bacteroides sartorii sp. nov.. Arch Microbiol 192:427–435 [View Article][PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  5. Kitahara M., Tsuchida S., Kawasumi K., Amao H., Sakamoto M., Benno Y., Ohkuma M. 2011; Bacteroides chinchillae sp. nov. and Bacteroides rodentium sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. Int J Syst Evol Microbiol 61:877–881 [View Article][PubMed]
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  7. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [View Article]
    [Google Scholar]
  8. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. other authors 2007; clustal w and clustal_x version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  10. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
    [Google Scholar]
  11. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [View Article][PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  13. Sakamoto M., Ohkuma M. 2010; Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods. J Med Microbiol 59:1293–1302 [View Article][PubMed]
    [Google Scholar]
  14. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. 2002; Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52:841–849 [View Article][PubMed]
    [Google Scholar]
  15. Sakamoto M., Suzuki N., Benno Y. 2010; hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes . Int J Syst Evol Microbiol 60:2984–2990 [View Article][PubMed]
    [Google Scholar]
  16. Shah H. N. 1992; The genus Bacteroides and related taxa. In The Prokaryotes, 2nd edn. pp. 3593–3607 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.035659-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035659-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error