1887

Abstract

Strains of the recently proposed species share more than 99.4 % 16S rRNA gene sequence similarity with the type strain of although these two species do not appear to be similar from their published descriptions. The aim of this study was to perform phenotypic and genetic analyses of both species to clarify their taxonomic position. JCM 16497 exhibited high gene sequence similarity with JCM 17136 (100 %) as well as JCM 16498 (100 %). The gene sequence analysis and levels of DNA–DNA relatedness observed demonstrated JCM 17136, JCM 16497, and JCM 16498 are members of a single species. Based on these data, we propose as a later heterotypic synonym of . An emended description of is provided.

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2012-06-01
2019-10-18
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References

  1. Clavel T. , Saalfrank A. , Charrier C. , Haller D. . ( 2010; ). Isolation of bacteria from mouse caecal samples and description of Bacteroides sartorii sp. nov.. Arch Microbiol 192:, 427–435. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. . ( 1985; ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  5. Kitahara M. , Tsuchida S. , Kawasumi K. , Amao H. , Sakamoto M. , Benno Y. , Ohkuma M. . ( 2011; ). Bacteroides chinchillae sp. nov. and Bacteroides rodentium sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. . Int J Syst Evol Microbiol 61:, 877–881. [CrossRef] [PubMed]
    [Google Scholar]
  6. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  7. Kuykendall L. D. , Roy M. A. , O’Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  8. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  9. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  10. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  11. Saito H. , Miura K. I. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  12. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  13. Sakamoto M. , Ohkuma M. . ( 2010; ). Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods. . J Med Microbiol 59:, 1293–1302. [CrossRef] [PubMed]
    [Google Scholar]
  14. Sakamoto M. , Suzuki M. , Umeda M. , Ishikawa I. , Benno Y. . ( 2002; ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52:, 841–849. [CrossRef] [PubMed]
    [Google Scholar]
  15. Sakamoto M. , Suzuki N. , Benno Y. . ( 2010; ). hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes . . Int J Syst Evol Microbiol 60:, 2984–2990. [CrossRef] [PubMed]
    [Google Scholar]
  16. Shah H. N. . ( 1992; ). The genus Bacteroides and related taxa. . In The Prokaryotes, , 2nd edn., pp. 3593–3607. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  17. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
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