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Abstract

A Gram-stain-negative, moderately halophilic, strictly aerobic bacterium, designated YIM 95161, was isolated from brine of a salt well in Yunnan province, China, and subjected to a taxonomic study using a polyphasic approach. Cells of strain YIM 95161 were short rods, approximately 0.9–1.4 µm long and 0.4–0.6 µm wide. Strain YIM 95161 grew at 15–40 °C (optimum, 25–30 °C), 6–29 % (w/v) NaCl (optimum, 14–19 %) and at pH 5.0–8.0 (optimum, pH 7.0). The predominant isoprenoid quinone was Q-8. The major fatty acids (>10 %) were summed feature 8 (Cω6 andor Cω7) and C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unknown phosphoglycolipid and two unknown lipids. The DNA G+C content was 69.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 95161 was a member of the genus and exhibited sequence similarities of 96.7 %, 95.6 % and 95.4 % to E1L3A, CL-ES53 and EPR70, respectively. On the basis of phylogenetic, physiological and chemotaxonomic analysis, strain YIM 95161 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 95161 ( = CCTCC AB 2011132 = JCM 17431).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31000001 and 30860002)
  • International Cooperation Research Program of Yunnan Province (Award 2009AC017)
  • China Postdoctoral Science Foundation (Award 20090461410, 201104661)
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2012-09-01
2021-10-22
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