1887

Abstract

A Gram-stain-negative, moderately halophilic, strictly aerobic bacterium, designated YIM 95161, was isolated from brine of a salt well in Yunnan province, China, and subjected to a taxonomic study using a polyphasic approach. Cells of strain YIM 95161 were short rods, approximately 0.9–1.4 µm long and 0.4–0.6 µm wide. Strain YIM 95161 grew at 15–40 °C (optimum, 25–30 °C), 6–29 % (w/v) NaCl (optimum, 14–19 %) and at pH 5.0–8.0 (optimum, pH 7.0). The predominant isoprenoid quinone was Q-8. The major fatty acids (>10 %) were summed feature 8 (Cω6 andor Cω7) and C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unknown phosphoglycolipid and two unknown lipids. The DNA G+C content was 69.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 95161 was a member of the genus and exhibited sequence similarities of 96.7 %, 95.6 % and 95.4 % to E1L3A, CL-ES53 and EPR70, respectively. On the basis of phylogenetic, physiological and chemotaxonomic analysis, strain YIM 95161 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 95161 ( = CCTCC AB 2011132 = JCM 17431).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31000001 and 30860002)
  • International Cooperation Research Program of Yunnan Province (Award 2009AC017)
  • China Postdoctoral Science Foundation (Award 20090461410, 201104661)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035584-0
2012-09-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2174.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035584-0&mimeType=html&fmt=ahah

References

  1. Antunes A., Eder W., Fareleira P., Santos H., Huber R. 2003; Salinisphaera shabanensis gen. nov., sp. nov., a novel, moderately halophilic bacterium from the brine-seawater interface of the Shaban Deep, Red Sea. Extremophiles 7:29–34[PubMed]
    [Google Scholar]
  2. Bae G. D., Hwang C. Y., Kim H. M., Cho B. C. 2010; Salinisphaera dokdonensis sp. nov., isolated from surface seawater. Int J Syst Evol Microbiol 60:680–685 [View Article][PubMed]
    [Google Scholar]
  3. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
    [Google Scholar]
  4. Cerny G. 1978; Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:113–122 [View Article]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  6. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [View Article]
    [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  8. Crespo-Medina M., Chatziefthimiou A., Cruz-Matos R., Pérez-Rodríguez I., Barkay T., Lutz R. A., Starovoytov V., Vetriani C. 2009; Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultatively autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera . Int J Syst Evol Microbiol 59:1497–1503 [View Article][PubMed]
    [Google Scholar]
  9. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. . Int J Syst Evol Microbiol 51:357–363[PubMed]
    [Google Scholar]
  10. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  12. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  13. Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010; New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321 [View Article][PubMed]
    [Google Scholar]
  14. Leifson E. 1960 Atlas of Bacterial Flagellation London: Academic Press;
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  19. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  20. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp. 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Philips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  23. Tang S. K., Tian X. P., Zhi X. Y., Cai M., Wu J. Y., Yang L. L., Xu L. H., Li W. J. 2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae. . Int J Syst Evol Microbiol 58:2075–2080 [View Article][PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  25. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey’s Manual of Systematic Bacteriology vol. 4 pp. 2463–2468 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.035584-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035584-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error