1887

Abstract

A novel strain of Gram-staining-positive bacterium, designated HI08-69, was isolated from the rhizosphere of a mangrove on Iriomote Island, Japan, and its taxonomic position was investigated by a polyphasic approach. The strain had peptidoglycan of the A4α type, with lysine as the diagnostic diamino acid. The predominant menaquinone was demethylmenaquinone DMK-9(H) and the major fatty acids were anteiso-C, anteiso-C and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified polar lipid but minor amounts of other polar lipids were also detected. The genomic DNA G+C content of strain HI08-69 was 71.7 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, the novel strain and members of the genus formed a monophyletic cluster, with pairwise sequence similarities of 95.6–96.8 %. However, strain HI08-69 was clearly distinguishable from all established members of the genus in terms of several chemotaxonomic characteristics. On the basis of the phenotypic and genotypic characteristics, strain HI08-69 represents a novel species in a new genus for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is HI08-69 ( = NBRC 105856 = DSM 24868).

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2012-08-01
2020-01-27
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References

  1. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  2. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  3. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Fernández-Garayzábal J. F., Dominguez L., Pascual C., Jones D., Collins M. D.. ( 1995;). Phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of Sanguibacter gen. nov.. Lett Appl Microbiol 20:, 69–75. [CrossRef][PubMed]
    [Google Scholar]
  5. Finster K. W., Herbert R. A., Kjeldsen K. U., Schumann P., Lomstein B. A.. ( 2009;). Demequina lutea sp. nov., isolated from a high Arctic permafrost soil. . Int J Syst Evol Microbiol 59:, 649–653. [CrossRef][PubMed]
    [Google Scholar]
  6. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Hamada M., Iino T., Iwami T., Harayama S., Tamura T., Suzuki K.. ( 2010;). Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.. J Gen Appl Microbiol 56:, 427–436. [CrossRef][PubMed]
    [Google Scholar]
  8. Matsumoto A., Nakai K., Morisaki K., Ōmura S., Takahashi Y.. ( 2010;). Demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin. . Int J Syst Evol Microbiol 60:, 1206–1209. [CrossRef][PubMed]
    [Google Scholar]
  9. Nozawa Y., Sakai N., Arai K., Kawasaki Y., Harada K.. ( 2007;). Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey’s method. . J Microbiol Methods 70:, 306–311. [CrossRef][PubMed]
    [Google Scholar]
  10. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  11. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  12. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  13. Schumann P., Kämpfer P., Busse H. J., Evtushenko L. I..Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes ( 2009;). Proposed minimal standards for describing new genera and species of the suborder Micrococcineae. . Int J Syst Evol Microbiol 59:, 1823–1849. [CrossRef][PubMed]
    [Google Scholar]
  14. Stackebrandt E., Breymann S., Steiner U., Prauser H. N. W., Weiss N., Schumann P.. ( 2002;). Re-evaluation of the status of the genus Oerskovia, reclassification of Promicromonospora enterophila (Jáger et al. 1983) as Oerskovia enterophila comb. nov. and description of Oerskovia jenensis sp. nov. and Oerskovia paurometabola sp. nov.. Int J Syst Evol Microbiol 52:, 1105–1111. [CrossRef][PubMed]
    [Google Scholar]
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  17. Ue H., Matsuo Y., Kasai H., Yokota A.. ( 2011;). Demequina globuliformis sp. nov., Demequina oxidasica sp. nov. and Demequina aurantiaca sp. nov., actinobacteria isolated from marine environments, and proposal of Demequinaceae fam. nov.. Int J Syst Evol Microbiol 61:, 1322–1329. [CrossRef][PubMed]
    [Google Scholar]
  18. Yi H., Schumann P., Chun J.. ( 2007;). Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov.. Int J Syst Evol Microbiol 57:, 151–156. [CrossRef][PubMed]
    [Google Scholar]
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