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Abstract

A Gram-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, CH15-1, was isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the late-blooming period of cyanobacteria. Strain CH15-1 grew optimally at pH 7.0 and 30 °C. A phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain CH15-1 belongs to the genus with the similarity range from 92.6–97.4 % and is closely related to YC6267 (97.4 %), TR7-09 (95.4 %), CF5-1 (94.7 %), CC-JY-1 (94.6 %) and HO3-R19 (92.6 %). However, the DNA–DNA hybridization between strain CH15-1 and the closest strain, YC6267, was 8.9–12.9 %. The DNA G+C content was 63.9 mol% compared to YC626, 65.8 mol%. Strain CH15-1 included Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. The major fatty acids (>5 %) were iso-C, iso-C, iso-C, iso-C 3-OH, iso-C and summed feature 9 (iso-Cω9 and/or C 10-methyl). On the basis of phylogenetic, phenotypic and genetic data, strain CH15-1 was classified in the genus as a member of a novel species, for which the name sp. nov. is proposed. The type strain is CH15-1 ( = KCTC 23553 = DSM 24763).

Funding
This study was supported by the:
  • , Advanced Biomass Research Center
  • , Global Frontier Program
  • , Korean Ministry of Education, Science & Technology
  • , CAER (Center for Aquatic Ecosystem Restoration
  • , Eco-STAR
  • , Korean Ministry of Environment
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2013-02-01
2020-12-04
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References

  1. Aslam Z., Park J. H., Kim S. W., Jeon C. O., Chung Y. R. ( 2009 ). Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov.. Int J Syst Evol Microbiol 59, 29672972. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. ( 1995 ). Current Protocols in Molecular Biology. New York:: Wiley;.
    [Google Scholar]
  3. Chen F., Shi Z., Wang G. ( 2012 ). Arenimonas metalli sp. nov., isolated from an iron mine. . Int J Syst Evol Microbiol 62, 17441749. [CrossRef] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid- deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  6. Gomori G. ( 1955 ). Preparation of buffer for use in enzyme studies. . Methods Enzymol 1, 138146. [CrossRef]
    [Google Scholar]
  7. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  8. Jin L., Kim K. K., Im W. T., Yang H. C., Lee S. T. ( 2007 ). Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae . . Int J Syst Evol Microbiol 57, 18761880. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  11. Kumar S., Tamura K., Nei M. ( 2004 ). mega3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. . Brief Bioinform 5, 150163. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kwon S. W., Kim B. Y., Weon H. Y., Baek Y. K., Go S. J. ( 2007 ). Arenimonas donghaensis gen. nov., sp. nov., isolated from seashore sand. . Int J Syst Evol Microbiol 57, 954958. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [CrossRef]
    [Google Scholar]
  15. Oh H. M., Ahn C. Y., Lee J. W., Chon T. S., Choi K. H., Park Y. S. ( 2007 ). Community patterning and identification of predominant factors in algal bloom in Daechung Reservoir (Korea) using artificial neural networks. . Ecol Modell 203, 109118. [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  18. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [CrossRef]
    [Google Scholar]
  19. Tarrand J. J., Gröschel D. H. M. ( 1982 ). Rapid, modified oxidase test for oxidase-variable bacterial isolates. . J Clin Microbiol 16, 772774.[PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  22. Young C. C., Kämpfer P., Ho M. J., Busse H. J., Huber B. E., Arun A. B., Shen F. T., Lai W. A., Rekha P. D. ( 2007 ). Arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. . Int J Syst Evol Microbiol 57, 27902793. [CrossRef] [PubMed]
    [Google Scholar]
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