Reclassification of and as later subjective synonyms of Free

Abstract

Characterization and identification of strain CW 1 ( = JCM 17161) isolated from corn silage were performed. Strain CW 1 was a Gram-positive, catalase-negative and homofermentative rod that produced the -form of lactic acid. This strain exhibited more than 99.6 % 16S rRNA gene sequence similarity and greater than 82 % DNA–DNA reassociation with type strains of , and . To clarify the taxonomic positions of these type strains, phenotypic characterization, 16S rRNA gene sequencing, ribotyping and DNA–DNA relatedness were examined. The three type strains displayed different -arabinose, lactose, melibiose, melezitose, raffinose and -acetyl-β-glucosaminidase fermentation patterns. Phylogenetic analysis showed that is a closer neighbour of and , sharing 99.5–99.9 % 16S rRNA gene sequence similarity, which was confirmed by the high DNA–DNA relatedness (≥82 %) between JCM 10415, JCM 16180 and JCM 10707. Therefore, it is proposed that and should be reclassified as later synonyms of

Funding
This study was supported by the:
  • Application of Nuclear Techniques in Agriculture (Award 200803034)
  • Chinese Ministry of Agriculture, the Super Wheat Breeding and Demonstration (Award 081100110500)
  • Henan Province and the State Scholarship Fund (Award 2009704013)
  • China Scholarship Council
  • Project of Exploration, and the Introduction of Microbial Genetic Resources in Foreign Countries from National Institute of Agro biological Science of Japan
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035329-0
2012-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/10/2383.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035329-0&mimeType=html&fmt=ahah

References

  1. Cai Y. 1999; Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation. J Dairy Sci 82:2466–2471 [View Article][PubMed]
    [Google Scholar]
  2. Cai Y., Okada H., Mori H., Benno Y., Nakase T. 1999; Lactobacillus paralimentarius sp. nov., isolated from sourdough. Int J Syst Bacteriol 49:1451–1455 [View Article][PubMed]
    [Google Scholar]
  3. Cai Y., Yang J., Pang H., Kitahara M. 2011; Lactococcus fujiensis sp. nov., a lactic acid bacterium isolated from vegetable matter. Int J Syst Evol Microbiol 61:1590–1594 [View Article][PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  5. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. 1992 International Code of Nomenclature of Bacteria (1990 Revision): Bacteriological Code Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Mañes-Lázaro R., Ferrer S., Rodas A. M., Urdiain M., Pardo I. 2008; Lactobacillus bobalius sp. nov., a lactic acid bacterium isolated from Spanish Bobal grape must. Int J Syst Evol Microbiol 58:2699–2703 [View Article][PubMed]
    [Google Scholar]
  7. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [View Article][PubMed]
    [Google Scholar]
  8. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [View Article][PubMed]
    [Google Scholar]
  9. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  10. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  11. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  12. Yoon J. H., Kang S. S., Mheen T. I., Ahn J. S., Lee H. J., Kim T. K., Park C. S., Kho Y. H., Kang K. H., Park Y. H. 2000; Lactobacillus kimchii sp. nov., a new species from kimchi. Int J Syst Evol Microbiol 50:1789–1795[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035329-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035329-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed