1887

Abstract

Characterization and identification of strain CW 1 ( = JCM 17161) isolated from corn silage were performed. Strain CW 1 was a Gram-positive, catalase-negative and homofermentative rod that produced the -form of lactic acid. This strain exhibited more than 99.6 % 16S rRNA gene sequence similarity and greater than 82 % DNA–DNA reassociation with type strains of , and . To clarify the taxonomic positions of these type strains, phenotypic characterization, 16S rRNA gene sequencing, ribotyping and DNA–DNA relatedness were examined. The three type strains displayed different -arabinose, lactose, melibiose, melezitose, raffinose and -acetyl-β-glucosaminidase fermentation patterns. Phylogenetic analysis showed that is a closer neighbour of and , sharing 99.5–99.9 % 16S rRNA gene sequence similarity, which was confirmed by the high DNA–DNA relatedness (≥82 %) between JCM 10415, JCM 16180 and JCM 10707. Therefore, it is proposed that and should be reclassified as later synonyms of

Funding
This study was supported by the:
  • Application of Nuclear Techniques in Agriculture (Award 200803034)
  • Chinese Ministry of Agriculture, the Super Wheat Breeding and Demonstration (Award 081100110500)
  • Henan Province and the State Scholarship Fund (Award 2009704013)
  • China Scholarship Council
  • Project of Exploration, and the Introduction of Microbial Genetic Resources in Foreign Countries from National Institute of Agro biological Science of Japan
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2012-10-01
2021-05-19
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