1887

Abstract

A novel methane-oxidizing bacterium, strain Fw12E-Y, was isolated from floodwater of a rice paddy field in Japan. Cells of strain Fw12E-Y were Gram-negative, motile rods with a single polar flagellum and type I intracytoplasmic membrane arrangement. The strain grew only on methane or methanol as sole carbon and energy source. It was able to grow at 10–40 °C (optimum 30 °C), at pH 5.5–7.0 (optimum 6.5) and with 0–0.1 % (w/w) NaCl (no growth above 0.5 % NaCl). 16S rRNA gene sequence analysis showed that strain Fw12E-Y is related most closely to members of the genus , but at low levels of similarity (95.0–95.4 %). Phylogenetic analysis of and genes indicated that the strain belongs to the genus (97 and 92 % deduced amino acid sequence identities to S1, respectively). The DNA G+C content of strain Fw12E-Y was 57.1 mol%. Chemotaxonomic data regarding the major quinone (MQ-8) and major fatty acids (C and C) also supported its affiliation to the genus . Based on phenotypic, genomic and phylogenetic data, strain Fw12E-Y is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Fw12E-Y ( = JCM 16701 = NBRC 105905 = NCIMB 14606).

Funding
This study was supported by the:
  • Japan Society for the Promotion of Sciences
  • ESPEC Foundation for Global Environment Research and Technology (Charitable Trust)
  • Chubu Science and Technology Center
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2012-08-01
2021-10-19
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