A novel actinomycete, designated strain PT708T, was isolated from cave soil collected in Pha Tup Cave Forest Park, Nan province, Thailand. It produced compounds with antimicrobial and anticancer activities. Its chemotaxonomic properties were consistent with those of members of the genus Nonomuraea. The major menaquinone was MK-9(H4), with minor amounts of MK-9(H6), MK-9(H2), MK-10(H2) and MK-8(H4). The polar lipid profile contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The major fatty acids were iso-C16 : 0, 10-methyl C17 : 0, C16 : 0 and C17 : 1ω6c. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PT708T belonged to the genus Nonomuraea and was most closely related to Nonomuraea rhizophila YIM 67092T (98.50 % sequence similarity) and Nonomuraea rosea GW 12687T (98.30 %). The genomic DNA G+C content of strain PT708T was 73.3 mol%. Unlike the recognized members of the genus Nonomuraea, the novel strain formed single spores at the tips of aerial hyphae. Based on the phenotypic, phylogenetic and genotypic evidence, strain PT708T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea monospora sp. nov. is proposed. The type strain is PT708T ( = TISTR 1910T = JCM 16114T).
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Funding
This study was supported by the:
Nanotechnology Research Fund, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
Division of Research Administration, Naresuan University, Phitsanulok, Thailand
ChibaS.,
SuzukiM.,
AndoK.1999; Taxonomic re-evaluation of ‘Nocardiopsis’ sp. K-252T ( = NRRL 15532T): a proposal to transfer this strain to the genus Nonomuraea as Nonomuraea longicatena sp. nov.. Int J Syst Bacteriol 49:1623–1630 [View Article][PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
FischerA.,
KroppenstedtR. M.,
StackebrandtE.1983; Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis
. J Gen Microbiol 129:3433–3446[PubMed]
GyobuY.,
MiyadohS.2001; Proposal to transfer Actinomadura carminata to a new subspecies of the genus Nonomuraea as Nonomuraea roseoviolacea subsp. carminata comb. nov.. Int J Syst Evol Microbiol 51:881–889 [View Article][PubMed]
HayakawaM.,
NonomuraH.1987; Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [View Article]
HopwoodD. A.,
BibbM. J.,
ChaterK. F.,
KieserT.,
BrutonC. J.,
KieserH. M.,
LydiateD. J.,
SmithC. P.,
WardJ. M.,
SchrempfH.1985Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: The John Innes Foundation;
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
KroppenstedtR. M.,
GoodfellowM.1991; The family Thermomonosporaceae
. In The Prokaryotes, 2nd edn. pp. 1085–1114 Edited by
BalowsA.,
TrüperH. G.,
DworkinM.,
HarderW.,
SchleiferK. H.
New York: Springer;
KroppenstedtR. M.,
StackebrandtE.,
GoodfellowM.1990; Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora
. Syst Appl Microbiol 13:148–160 [View Article]
LaneD. J.1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–148 Edited by
StackebrandtE.,
GoodfellowM.
Chichester: Wiley;
Le RoesM.,
MeyersP. R.2008; Nonomuraea candida sp. nov., a new species from South African soil. Antonie van Leeuwenhoek 93:133–139 [View Article][PubMed]
LechevalierM. P.,
LechevalierH. A.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E.,
CollinsM. D.,
GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47:87–95 [View Article]
NakaewN.,
Pathom-areeW.,
LumyongS.2009; Generic diversity of rare actinomycetes from Thai cave soils and their possible use as new bioactive compounds. Actinomycetologica 23:21–26 [View Article]