sp. nov., an actinomycete isolated from cave soil in Thailand, and emended description of the genus Free

Abstract

A novel actinomycete, designated strain PT708, was isolated from cave soil collected in Pha Tup Cave Forest Park, Nan province, Thailand. It produced compounds with antimicrobial and anticancer activities. Its chemotaxonomic properties were consistent with those of members of the genus . The major menaquinone was MK-9(H), with minor amounts of MK-9(H), MK-9(H), MK-10(H) and MK-8(H). The polar lipid profile contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The major fatty acids were iso-C, 10-methyl C, C and Cω6. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PT708 belonged to the genus and was most closely related to YIM 67092 (98.50 % sequence similarity) and GW 12687 (98.30 %). The genomic DNA G+C content of strain PT708 was 73.3 mol%. Unlike the recognized members of the genus , the novel strain formed single spores at the tips of aerial hyphae. Based on the phenotypic, phylogenetic and genotypic evidence, strain PT708 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PT708 ( = TISTR 1910 = JCM 16114).

Funding
This study was supported by the:
  • Nanotechnology Research Fund, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand
  • Division of Research Administration, Naresuan University, Phitsanulok, Thailand
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2012-12-01
2024-03-28
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