1887

Abstract

A novel actinomycete, designated strain PT708, was isolated from cave soil collected in Pha Tup Cave Forest Park, Nan province, Thailand. It produced compounds with antimicrobial and anticancer activities. Its chemotaxonomic properties were consistent with those of members of the genus . The major menaquinone was MK-9(H), with minor amounts of MK-9(H), MK-9(H), MK-10(H) and MK-8(H). The polar lipid profile contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The major fatty acids were iso-C, 10-methyl C, C and Cω6. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PT708 belonged to the genus and was most closely related to YIM 67092 (98.50 % sequence similarity) and GW 12687 (98.30 %). The genomic DNA G+C content of strain PT708 was 73.3 mol%. Unlike the recognized members of the genus , the novel strain formed single spores at the tips of aerial hyphae. Based on the phenotypic, phylogenetic and genotypic evidence, strain PT708 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PT708 ( = TISTR 1910 = JCM 16114).

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2012-12-01
2019-10-15
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References

  1. Ara I., Kudo T., Matsumoto A., Takahashi Y., Omura S.. ( 2007a;). Nonomuraea bangladeshensis sp. nov. and Nonomuraea coxensis sp. nov.. Int J Syst Evol Microbiol 57:, 1504–1509. [CrossRef][PubMed]
    [Google Scholar]
  2. Ara I., Kudo T., Matsumoto A., Takahashi Y., Omura S.. ( 2007b;). Nonomuraea maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere mud. . J Gen Appl Microbiol 53:, 159–166. [CrossRef][PubMed]
    [Google Scholar]
  3. Athalye M., Goodfellow M., Lacey J., White R. P.. ( 1985;). Numerical classification of Actinomadura and Nocardiopsis. . Int J Syst Bacteriol 35:, 86–98. [CrossRef]
    [Google Scholar]
  4. Chiba S., Suzuki M., Ando K.. ( 1999;). Taxonomic re-evaluation of ‘Nocardiopsis’ sp. K-252T ( = NRRL 15532T): a proposal to transfer this strain to the genus Nonomuraea as Nonomuraea longicatena sp. nov.. Int J Syst Bacteriol 49:, 1623–1630. [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  6. Fischer A., Kroppenstedt R. M., Stackebrandt E.. ( 1983;). Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis. . J Gen Microbiol 129:, 3433–3446.[PubMed]
    [Google Scholar]
  7. Goodfellow M.. ( 1971;). Numerical taxonomy of some nocardioform bacteria. . J Gen Microbiol 69:, 33–80. [CrossRef][PubMed]
    [Google Scholar]
  8. Gyobu Y., Miyadoh S.. ( 2001;). Proposal to transfer Actinomadura carminata to a new subspecies of the genus Nonomuraea as Nonomuraea roseoviolacea subsp. carminata comb. nov.. Int J Syst Evol Microbiol 51:, 881–889. [CrossRef][PubMed]
    [Google Scholar]
  9. Hasegawa T., Takizawa M., Tanida S.. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H.. ( 1987;). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  11. Hayakawa M., Momose Y., Kajiura T., Yamazaki T., Tamura T., Hatano K., Nonomura H.. ( 1995;). A selective isolation method for Actinomadura viridis in soil. . J Ferment Bioeng 79:, 287–289. [CrossRef]
    [Google Scholar]
  12. Hickey R. J., Tresner H. D.. ( 1952;). A cobalt-containing medium for sporulation of Streptomyces species. . J Bacteriol 64:, 891–892.[PubMed]
    [Google Scholar]
  13. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H.. ( 1985;). Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich:: The John Innes Foundation;.
    [Google Scholar]
  14. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  15. Kämpfer P., Kroppenstedt R. M., Grün-Wollny I.. ( 2005;). Nonomuraea kuesteri sp. nov.. Int J Syst Evol Microbiol 55:, 847–851. [CrossRef][PubMed]
    [Google Scholar]
  16. Kämpfer P., Busse H. J., Tindall B. J., Nimtz M., Grün-Wollny I.. ( 2010;). Nonomuraea rosea sp. nov.. Int J Syst Evol Microbiol 60:, 1118–1124. [CrossRef][PubMed]
    [Google Scholar]
  17. Kroppenstedt R. M., Goodfellow M.. ( 1991;). The family Thermomonosporaceae. . In The Prokaryotes, , 2nd edn., pp. 1085–1114. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H... New York:: Springer;.
    [Google Scholar]
  18. Kroppenstedt R. M., Stackebrandt E., Goodfellow M.. ( 1990;). Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora. . Syst Appl Microbiol 13:, 148–160. [CrossRef]
    [Google Scholar]
  19. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–148. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  20. Le Roes M., Meyers P. R.. ( 2008;). Nonomuraea candida sp. nov., a new species from South African soil. . Antonie van Leeuwenhoek 93:, 133–139. [CrossRef][PubMed]
    [Google Scholar]
  21. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  22. Li J., Zhao G.-Z., Huang H.-Y., Zhu W.-Y., Lee J.-C., Xu L.-H., Kim C.-J., Li W.-J.. ( 2011;). Nonomuraea endophytica sp. nov., an endophytic actinomycete isolated from Artemisia annua L.. Int J Syst Evol Microbiol 61:, 757–761. [CrossRef][PubMed]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Microbiol 47:, 87–95. [CrossRef]
    [Google Scholar]
  25. Mundie D. A.. ( 1995;). The NBS/ISCC Color System. . http://www.dodomagnifico.com/Colors/NBS.html.
    [Google Scholar]
  26. Nakaew N., Pathom-aree W., Lumyong S.. ( 2009;). Generic diversity of rare actinomycetes from Thai cave soils and their possible use as new bioactive compounds. . Actinomycetologica 23:, 21–26. [CrossRef]
    [Google Scholar]
  27. Poschner J., Kroppenstedt R. M., Fischer A., Stackebrandt E.. ( 1985;). DNA-DNA reassociation and chemotaxonomic studies on Actinomadura, Microbispora, Microtetraspora, Micropolyspora and Nocardiopsis. . Syst Appl Microbiol 6:, 264–270. [CrossRef]
    [Google Scholar]
  28. Pridham T. G., Gottlieb D.. ( 1948;). The utilization of carbon compounds by some Actinomycetales as an aid for species determination. . J Bacteriol 56:, 107–114.
    [Google Scholar]
  29. Quintana E., Maldonado L., Goodfellow M.. ( 2003;). Nonomuraea terrinata sp. nov., a novel soil actinomycete. . Antonie van Leeuwenhoek 84:, 1–6. [CrossRef][PubMed]
    [Google Scholar]
  30. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  31. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  32. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  33. Skehan P., Storeng R., Scudiero D., Monks A., McMahon J., Vistica D., Warren J. T., Bokesch H., Kenney S., Boyd M. R.. ( 1990;). New colorimetric cytotoxicity assay for anticancer-drug screening. . J Natl Cancer Inst 82:, 1107–1112. [CrossRef][PubMed]
    [Google Scholar]
  34. Stackebrandt E., Ebers J.. ( 2006;). Taxonomic parameters revised: tarnished gold standards. . Microbiol Today 4:, 152–155.
    [Google Scholar]
  35. Stackebrandt E., Wink J., Steiner U., Kroppenstedt R. M.. ( 2001;). Nonomuraea dietzii sp. nov.. Int J Syst Evol Microbiol 51:, 1437–1441.[PubMed]
    [Google Scholar]
  36. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  37. Waksman S. A.. ( 1967;). The Actinomycetes: a Summary of Current Knowledge. New York:: Ronald Press;.
    [Google Scholar]
  38. Wang F., Xu X.-X., Qu Z., Wang C., Lin H.-P., Xie Q.-Y., Ruan J. S., Sun M., Hong K.. ( 2011;). Nonomuraea wenchangensis sp. nov., isolated from mangrove rhizosphere soil. . Int J Syst Evol Microbiol 61:, 1304–1308. [CrossRef][PubMed]
    [Google Scholar]
  39. Xi L., Zhang L., Ruan J., Huang Y.. ( 2011;). Nonomuraea maritima sp. nov., isolated from coastal sediment. . Int J Syst Evol Microbiol 61:, 2740–2744. [CrossRef][PubMed]
    [Google Scholar]
  40. Zhang Z. S., Wang Y., Ruan J. S.. ( 1998;). Reclassification of Thermomonospora and Microtetraspora. . Int J Syst Bacteriol 48:, 411–422. [CrossRef][PubMed]
    [Google Scholar]
  41. Zhao G.-Z., Li J., Huang H.-Y., Zhu W.-Y., Xu L.-H., Li W.-J.. ( 2011;). Nonomuraea rhizophila sp. nov., an actinomycete isolated from rhizosphere soil. . Int J Syst Evol Microbiol 61:, 2141–2145. [CrossRef][PubMed]
    [Google Scholar]
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